The Effect of Steroids on the Regulation of Major Nonlymphoid Tissue1 Histocompatibility Complex-Class I1 Expression On

Ruers, T.J.M.; Leeuwenberg, J. F. M.; Spronken, E. E. M.; Linden, C. J. Van Der; Buurman, Wim A.

doi:10.1097/00007890-198903000-00019

Cited by 8 publications

(6 citation statements)

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“…This effect was dose‐dependent, achieving complete inhibition of cytokine‐induced class II expression at higher doses. Our findings are in agreement with a previous report using prednisolone in a model of colon cancer cells 20 . These findings help determine the mechanisms by which class II molecule expression is regulated in the intestinal epithelium.…”

Section: Discussionsupporting

confidence: 93%

“…Our ®ndings are in agreement with a previous report using prednisolone in a model of colon cancer cells. 20 These ®ndings help determine the mechanisms by which class II molecule expression is regulated in the intestinal epithelium. The signi®cantly increased MHC class II expression encountered in various in¯ammatory conditions is dramatically reduced after corticosteroid treatment.…”

Section: Mature Intestinal Epithelial Cells Normally Express Mhc Clasmentioning

confidence: 99%

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Dexamethasone inhibits IFNγ‐induced MHC class II expression of intestinal epithelial cells independently of the TGF‐β1 regulatory pathway

Ruemmele

Dionne

Lévy

et al. 1999

Aliment Pharmacol Ther

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Section: Discussionsupporting

confidence: 93%

Section: Mature Intestinal Epithelial Cells Normally Express Mhc Clasmentioning

confidence: 99%

Dexamethasone inhibits IFNγ‐induced MHC class II expression of intestinal epithelial cells independently of the TGF‐β1 regulatory pathway

Ruemmele

Dionne

Lévy

et al. 1999

Aliment Pharmacol Ther

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“…The VXM is scored as positive predicated on low‐level anti‐HLA antibody, but low or weak HLA expression on target cells may yield a negative physical crossmatch. (c) Drugs including statins and steroids can downregulate the surface expression of HLA adding additional complexities to the interpretation of the FCXM . Due to the possibility that HLA surface expression may be compromised resulting in a false negative FCXM, one must reconsider the utility of a physical crossmatch when there exists low‐level DSAs.…”

Section: Complex Scenariosmentioning

confidence: 99%

“…(c) Drugs including statins and steroids can downregulate the surface expression of HLA adding additional complexities to the interpretation of the FCXM. 59,60 Due to the possibility that HLA surface expression may be compromised resulting in a false negative FCXM, one must reconsider the utility of a physical crossmatch when there exists low-level DSAs. The belief that a "negative" physical crossmatch confers a lower risk may not be valid in these instances.…”

Section: Scenario 4: Fcxm Negative/vxm Positivementioning

confidence: 99%

Out with the old, in with the new: Virtual versus physical crossmatching in the modern era

Morris

Sullivan

Krummey

et al. 2019

HLA

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The virtual crossmatch (VXM) is gaining acceptance as an alternative approach to assess donor:recipient compatibility prior to transplantation. In contrast to a physical crossmatch, the virtual crossmatch does not require viable donor cells but rather relies on complete HLA typing of the donor and current antibody assessment of the recipient. Thus, the VXM can be performed in minutes which allows for faster transplant decisions thereby increasing the likelihood that organs can be shipped across significant distances yet safely transplanted. Here, we present a brief review of the past 50 years of histocompatibility testing; from the original complement‐dependent cytotoxicity crossmatch in 1969 to the new era of molecular HLA typing, solid‐phase antibody testing and virtual crossmatching. These advancements have shaped a paradigm shift in our approach to transplantation. That is, foregoing a prospective physical crossmatch in favor of a VXM. In this review, we undertake an in‐depth analysis of the pros‐ and cons‐ of physical and virtual crossmatching.Finally, we provide objective data on the selected use of the VXM which demonstrate the value of a VXM in lieu of the traditional physical crossmatch for safe and efficient organ transplantation.

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“…Immunogenicity depends on the presence of minor and major histocompatibility complex (MHC) class I and II gene products on the cell surface. It has been shown that class II antigen expression is not stable, and various protocols may change the extent to which these antigens are expressed [1,2,16,19,22,24,25). It has also been demonstrated in studies on the heart and pancreas [3,21] that rat strains may be divided into Offprint requests to: M.Krzymanski, Department of Nephrology, Institute of Internat Medicine, Academy of Medicine, Al.…”

mentioning

confidence: 99%

The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival

Krzymański¹,

Waaga²,

Ulrichs³

et al. 1991

Transplant Int

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Abstract. To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.Keywords: Class II antigen blockade, rat, renal transplantation -Monoclonal antibody, dass II antigen blockadeRenal transplantation, rat, class II antigen blockadeThe immunogenicity of the graft and the immunological reactivity of the recipient are the main factors that determine allogeneic graft rejection. Immunogenicity depends on the presence of minor and major histocompatibility complex (MHC) class I and II gene products on the cell surface. It has been shown that class II antigen expression is not stable, and various protocols may change the extent to which these antigens are expressed [1,2,16,19,22,24,25). It has also been demonstrated in studies on the heart and pancreas [3,21] that rat strains may be divided into Offprint requests to: M.Krzymanski, Department of Nephrology, Institute of Internat Medicine, Academy of Medicine, Al. Przybyszewskiego 49, Poznaii 60-355, Poland two groups. One has a high density of interstitial dendritic cells (DC) that express class II antigens and the other a low density. For successful transplantationindifferent rat strain combinations, it may be advantageaus to know the dass II MHC antigen expression and to take it into account.To decrease the immunogenicity of the graft, various procedures have been used [4,9,13,14,17,18,23]. Evidence is accumulating to support the hypothesis that graft immunogenicity may be altered by pretreatment with anti-class II monoclonal antibodies. However, evidence of the effectiveness of perfusion procedures, as presented in the literature, is quite fragmentary.In the present study, MHC dass II antigen expression was investigated in rat renal grafts to analyze the effectiveness of the perfusion procedure in pretreating the graft and to define the optimum conditions for blocking dass II antigens expressed on DC. The efficacy of the method was assessed morphologically by the immunoperoxidase technique on renal tissue sections. Kidneys p...

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The Effect of Steroids on the Regulation of Major Nonlymphoid Tissue1 Histocompatibility Complex-Class I1 Expression On

Cited by 8 publications

References 0 publications

Dexamethasone inhibits IFNγ‐induced MHC class II expression of intestinal epithelial cells independently of the TGF‐β1 regulatory pathway

Dexamethasone inhibits IFNγ‐induced MHC class II expression of intestinal epithelial cells independently of the TGF‐β1 regulatory pathway

Out with the old, in with the new: Virtual versus physical crossmatching in the modern era

The influence of MHC class II antigen blockade by perfusion with a monoclonal antibody on rat renal graft survival

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