The effect of sporulation medium on the structure and heat resistance of spores of<i>Closridium bifermentans</i>

Waites, W. M.; Stansfield, Rosamund; Bayliss, Catherine E.

doi:10.1111/j.1574-6968.1979.tb03340.x

Cited by 15 publications

(2 citation statements)

References 16 publications

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“…From these results it is evident that the BC40 B. cereus spores, which were produced under conditions identical with those used for the others (BC20 and BC30) but by growth at 40°C, appear smaller and less resistant to heat than the others. Such a result confirms earlier work suggesting that variation in sporulation conditions changes the size and resistance of spores (Waites et al, 1979…”

Section: Effects Of Disinfectantsupporting

confidence: 92%

Dynamic light-scattering studies on the effect of heat and disinfectants on spores of Bacillus cereus

Molina-García

Harding

Pieri

et al. 1989

Biochemical Journal

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The relative stability of spores of Bacillus cereus grown at three different temperatures was examined by using quasi-elastic light scattering (q.l.s.) in conjunction with turbidity and scanning electron microscopy (s.e.m.). Cultures grown at 20, 30 and 40 degrees C (BC20, BC30 and BC40 respectively) were compared in terms of (i) their effective hydrodynamic radius, rH, as determined from q.l.s. and (ii) their gross morphology, as determined from s.e.m. The effects of autoclaving at 121.1 degrees C on both these properties was also examined. We observed (1) that cultures BC20 and BC30 appeared to have similar values for rH, whereas that of BC40 appeared some 50% higher, and (2) BC40 had a correspondingly much lower heat resistance (its structural integrity was lost after about 20 min autoclaving, whereas that of BC20 and BC30 was retained even after 80 min autoclaving). These data were in good agreement with independent measurements of heat-resistance coefficients. Changes in the hydrodynamic radius, polydispersity (both using q.l.s.) and turbidity were monitored with time on addition of the disinfectants sodium hypochlorite and peracetic acid; again BC40 appeared to have a lower resistance.

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Section: Effects Of Disinfectantsupporting

confidence: 92%

Dynamic light-scattering studies on the effect of heat and disinfectants on spores of Bacillus cereus

Molina-García

Harding

Pieri

et al. 1989

Biochemical Journal

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“…The composition of medium and the cultivation temperature affect structures and properties of spores (Hitchins et af., 1972;Waites et al, 1979;Bayliss et al, 1981 ;Foegeding & Fulp, 1988). To examine the effect of culture medium on surface hydrophobicity, B. megaterium QM B1551 and ATCC 33729, B. licheniformis, B. pumilus and B. cereus T were cultivated in supplemented nutrient broth and on tryptose yeast extract agar and the resulting spores were tested for their hydrophobicity.…”

Section: Comparison Of Surface Hydrophobicity Of Spores Of Various Bamentioning

confidence: 99%

Surface Hydiophobicity of Spores of Bacillus spp.

Koshikawa

Yamazaki²,

Yoshimi³

et al. 1989

Microbiology

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The surface hydrophobicity of 12 strains of Bacillus spp. was examined in a hexadecane-aqueous partition system. Mature and germinated spores of Bacillus megaterium QM B1551 transferred to the hexadecane layer, while vegetative and sporulating cells did not. Wild-type spores were more hydrophobic than spores of an exosporium-deficient mutant of B. megaterium QM B1551, although the mutant spores were shown to be hydrophobic to some extent by using increased volumes of hexadecane. This result suggests that the exosporium is more hydrophobic than the spore coat and that the surface hydrophobicity of spores depends mainly on components of the exosporium. The surface hydrophobicity of spores of nine other species of Bacillus was also examined, and spores having an exosporium were more hydrophobic than those lacking an exosporium. Thus measurement of the hydrophobicity of spores by the hexadecane partition method may provide a simple and rapid preliminary means of determining the presence or absence of an exosporium.

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Sporeforming Bacteria

Busta¹,

Foegeding²

1986

Advances in Meat Research

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The effect of sporulation medium on the structure and heat resistance of spores ofClosridium bifermentans

Cited by 15 publications

References 16 publications

Dynamic light-scattering studies on the effect of heat and disinfectants on spores of Bacillus cereus

Dynamic light-scattering studies on the effect of heat and disinfectants on spores of Bacillus cereus

Surface Hydiophobicity of Spores of Bacillus spp.

Sporeforming Bacteria

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