The effect of sperm DNA fragmentation index on assisted reproductive technology outcomes and its relationship with semen parameters and lifestyle

Yang, Hongyi; Li, Gang; Jin, Haixia; Guo, Yanjie; Sun, Yingpu

doi:10.21037/tau.2019.06.22

Cited by 93 publications

(96 citation statements)

References 39 publications

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“…Telli et al also reported a negative correlation between DFI and sperm motility (r = − 0.267, p = 0.043) [38]. Yang et al reported a negative correlation between DFI and sperm motility, concentration, and morphology (r = − 0.307, − 0.552, and − 0.620, respectively; all p < 0.01) [11]. This study also revealed a positive correlation between DFI and age, suggesting that DFI increased as the patient aged.…”

Section: Prospective 8 Highmentioning

confidence: 93%

“…An explanation for the reduction in DNA fragmentation after varicocelectomy lies in the proposed pathophysiology of DNA damage in varicocele patients. Sperm DNA fragmentation occurs during spermatogenesis and sperm maturation [11]. Hypoxia caused due to venous stasis and reflux results in an increase in reactive oxygen species (ROS).…”

Section: Prospective 8 Highmentioning

confidence: 99%

“…The sperm DNA fragmentation index (DFI) is a potential parameter for fertility investigation. DFI reflects the sperm DNA integrity and damage [11]. Studies have suggested that the degree of DNA fragmentation can predict the outcomes of ARTs.…”

Section: Introductionmentioning

confidence: 99%

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The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

Birowo

Wijaya

Atmoko

et al. 2020

Basic Clin. Androl.

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Background Varicocele is one of the most common causes of reversible male infertility, and 15% of the varicocele patients with normal semen analysis are diagnosed as infertile. According to the current guidelines, varicocelectomy is indicated based on abnormal sperm parameters and not abnormal DNA fragmentation index (DFI) values. Thus, in this study, we performed a meta-analysis of the effects of varicocelectomy on the DFI and other conventional sperm parameters, and determined whether DFI could be used to indicate varicocelectomy for varicocele patients. Results Through an electronic search of the PubMed, Scopus, EBSCO, and Cochrane databases, we included 7 prospective studies including a total of 289 patients in this meta-analysis. The results showed that varicocelectomy significantly reduced DNA fragmentation (mean difference: − 6.86; 95% confidence interval [CI]: − 10.04, − 3.69; p < 0.00001) and improved sperm concentration (mean difference: 9.59; 95% CI: 7.80, 11.38; p < 0.00001), progressive motility (mean difference: 8.66; 95% CI: 6.96, 10.36; p < 0.00001), and morphology (mean difference: 2.73; 95% CI: 0,65, 4.80; p = 0.01). Conclusion Varicocelectomy reduced DNA fragmentation and improved sperm concentration, progressive motility, and morphology. Additionally, the analysis showed that an abnormal DFI measurement should be considered as an indication for varicocelectomy.

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Section: Prospective 8 Highmentioning

confidence: 93%

Section: Prospective 8 Highmentioning

confidence: 99%

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The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

Birowo

Wijaya

Atmoko

et al. 2020

Basic Clin. Androl.

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“…5). The %DFI can be used to assess sperm DNA damage and can be directly associated with fertility [26,40].…”

Section: Discussionmentioning

confidence: 99%

Effects of cashew gum and nanoparticles on cooled stallion semen

et al. 2020

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Background Cryopreservation of stallion spermatozoa tends to cause plasma membrane damage due to the low ratio of cholesterol to phospholipids. Gums have been suggested as an alternative cryoprotectant to glycerol for stallion spermatozoa. Therefore, the present experiment was designed to verify whether the effect of addition of cashew gum (CG), or nanoparticles (NP) containing CG, to the extender before cooling on sperm quality in stallion semen. Ejaculates from 6 stallions were extended and split between six treatment groups (control, a-tocopherol [TOC], CG1, CG0.5, NP1 and NP0.5), stored in cryotubes at 4 °C. Results Aliquots were analysed by computer-assisted sperm motility analysis on the day of collection, and after 24 h and 48 h of cold storage. After 48 h, the total motility with NP1 (78.53 + 6.31%) was similar to control 85.79 + 6.31% at 0 h. The same pattern was observed for progressive motility. Membrane integrity assessed by flow cytometer was similar between control, TOC and G1 at all storage times. The DNA fragmentation in the control group increased at all time points, whereas chromatin integrity was maintained after 24 h in TOC and NP0.5 compared to 0 h. There was no increase in the proportion of live spermatozoa producing hydrogen peroxide, but there was a tendency for an increased proportion of spermatozoa in the live superoxide category in CG1 after 24 h cooled storage. Conclusions The addition of CG or CG-derived NP to extender for stallion semen was not harmful to the sperm cells.

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“…We did nevertheless observe single outlier measurements from several of the donors and a limitation of our study was that repeated analysis was only performed on eight donors. Repeated measurements on more individuals and linkage to changes in lifestyle are needed to establish if associations exist between lifestyle and the acrosomal status, as observed for some of the classical sperm parameters and sperm DNA fragmentation [33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity

Norup

Palme²,

Petersen

et al. 2020

JCM

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The acrosome of the spermatozoa is required for fertilization and in the raw ejaculate the percentage of viable acrosome-intact spermatozoa, the acrosomal status, is higher among men with good semen quality. Here we investigated if the acrosomal status of the processed semen preparations used at a fertility clinic can also be informative and whether it is associated with fecundity. The acrosomal status was measured by image cytometry on purified semen samples from couples during in vitro fertilization (IVF) (n = 99) and intracytoplasmic sperm injection (ICSI) (n = 107) treatment. Purified frozen-thawed donor samples were also analyzed (n = 199). In purified semen preparations the acrosomal status was significantly higher among sperm donors (p = 5.3 × 10−8) and men from IVF couples (p = 2.2 × 10−5) when compared to men from ICSI couples. A significant difference was also found between female, male and mixed factor infertility (p = 0.003). No association with lifestyle factors was found. In frozen-thawed donor samples, a significant positive (r = 0.16, p = 0.025) association with the number of pregnancies per sold straw was observed together with an area under the curve of 75.3%, when comparing the top and bottom deciles. Our results indicate that the acrosomal status may be a valuable parameter for personalizing fertility treatments and might be a good predictor of pregnancy success among normozoospermic men.

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The effect of sperm DNA fragmentation index on assisted reproductive technology outcomes and its relationship with semen parameters and lifestyle

Cited by 93 publications

References 39 publications

The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis

Effects of cashew gum and nanoparticles on cooled stallion semen

The Acrosomal Status of Density Purified Spermatozoa Differentiates Men from Couples in IVF and ICSI Treatment and Is Associated with Fecundity

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