The Effect of Specimen Preparation on Post‐Excision and Post‐Fixation Dimensions, Translation, and Distortion of Canine Cadaver Skin‐Muscle‐Fascia Specimens

Risselada, Marije; Mathews, Kyle G.; Griffith, Emily H.

doi:10.1111/vsu.12481

Cited by 18 publications

(18 citation statements)

References 18 publications

(32 reference statements)

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“…For best histopathologic margin reporting, it is equally important for the submitting veterinarian to handle and submit samples appropriately. Margin translation by the pathologist has been complicated by the decrease in size of tissue from the time of pre‐surgical measurement and at the time of histological evaluation . Unfortunately, at this time there is not an easy solution to the shrinkage phenomenon seen with surgical specimens.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, at this time there is not an easy solution to the shrinkage phenomenon seen with surgical specimens. One study, in an attempt to minimize the discrepancy between gross pre‐excision dimensions and post‐excision dimensions, suggested the clinician should suture the specimen during surgery before complete excision to improve alignment of the tissue planes without distorting the sample . Additionally, when applicable, surgical margins should be indicated by the submitting veterinarian with ink to aid the reviewing pathologist.…”

Section: Discussionmentioning

confidence: 99%

“…Margin translation by the pathologist has been complicated by the decrease in size of tissue from the time of pre-surgical measurement and at the time of histological evaluation. 25,[28][29][30] Unfortunately, at this time there is not an easy solution to the shrinkage phenomenon seen with surgical specimens.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of information presented within soft tissue sarcoma histopathology reports in the United States: 2012‐2015

Livaccari

Selmic

Reagan

et al. 2018

Vet Comparative Oncology

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Despite the existence of the American College of Veterinary Pathology guidelines for tumour biopsy specimens, anecdotally the authors' have seen inconsistency of reporting of information on the pathology report for canine soft tissue sarcomas (STSs). If crucial aspects are not reported this can result in slower or impeded patient care. This retrospective study evaluated 255 STS histopathology reports submitted from across the United States. Reports were evaluated by a single observer to assess for information contained in 5 main categories: patient history and signalment, gross and microscopic description, grading, histologic margins and the comments section. Inclusion criteria for histopathology reports included a final diagnosis of STS, having a microscopic description and resulting from the initial surgical resection. The majority of the reports stated the patient signalment (91.2%) and clinical history (90.8%). However, only 64.8% of the reports had a gross description of the specimen. Histologic margin description was present in 229 reports (91.6%), however, only 149 reports (59.6%) stated an objective measurement of these margins. Histologic classification was stated in 50.0% of the reports, while grade was given on 97.2% of the reports. Variability in histopathologic reporting including histologic margin description for resected canine STS was identified. Given surgical treatment is the mainstay for STS and histopathological assessment plays an important role in determination of whether additional surgery, radiation or chemotherapy is needed. Standardization or checklists like the American College of Pathology utilize may be helpful to ensure histopathologic characteristics are reported that may guide further treatment recommendations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of information presented within soft tissue sarcoma histopathology reports in the United States: 2012‐2015

Livaccari

Selmic

Reagan

et al. 2018

Vet Comparative Oncology

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“…Locally invasive malignancies are frequently treated by surgical excision, which improves clinical outcomes when histologically tumor‐free margins (HTFMs) are achieved . Recent studies have provided preliminary insight into the relationship between HTFM and intraoperative, en bloc excised, grossly normal surgical margins (GNSMs) . Improving our understanding of this relationship is central to creating an evidence‐based rationale for surgical planning and GNSM resection and is tightly coupled to patient morbidity and/or mortality …”

Section: Introductionmentioning

confidence: 99%

“…The latter is explained by microscopic infiltration of the GNSM, resulting in a narrower HTFM . Other relevant prior publications tested length reductions in normal (ie, non‐neoplastic) tissues, and may therefore not apply to tumor‐bearing specimens; in addition, retrospective studies have not been able to determine at which point of tissue processing did these changes occur …”

Section: Introductionmentioning

confidence: 99%

Reductions in margin length after excision of grade II mast cell tumors and grade I and II soft tissue sarcomas in dogs

et al. 2017

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Objective: Quantify changes in the circumferential lengths of surgical margins of resected canine mast cell tumors (MCT) and soft tissue sarcomas (STS) between the time of collection and histopathology.Study design: Prospective, hypothesis-driven, clinical study.Sample population: Two hundred and thirty-seven margins from 69 excised tumors (50 MCT and 19 STS) in 51 client-owned dogs. Methods:The lengths of surgical margins were recorded (eg, cranial, caudal, dorsal, and ventral) for each tumor at 5 time points: intraoperatively (in vivo), immediately after excision (ex vivo), after formalin fixation (postfixation), once mounted on glass slides (subgross), and as histologically tumor-free margins (HTFMs).Results: Compared to in vivo dimensions, the length of surgical margins at each processing step (ie, ex vivo, postfixation, subgross, and HTFM) was reduced by a median of 3.0, 5.0, 6.0, and 8.8 mm for MCT; 2.5, 2.0, 5.0, and 5.0 mm for STS. All processing steps resulted in significant reductions among MCT samples (P < .0001), except between postfixation vs subgross, and for STS samples (P < .0001), except between ex vivo vs postfixation and subgross vs HTFM. The maximum reduction in the total length of margins (from in vivo to HTFM) was 29.6 and 24.2 mm for MCT and STS, respectively.Conclusion: Surgical margin length reductions occur due to a combination of physical factors (eg, tissue elasticity, myofibril contraction, and histologic processing) and biological factors (eg, microscopic tumor infiltration into the grossly normal surgical margin).Clinical significance: These data provide information relevant to evidence-based surgical planning and may influence patient morbidity in the most commonly encountered cutaneous malignancies of dogs.

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Summary of Skin Reconstruction Options

Tillson

2024

Techniques in Small Animal Soft Tissue, Orthopedic, and Ophthalmic Surgery

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The Effect of Specimen Preparation on Post‐Excision and Post‐Fixation Dimensions, Translation, and Distortion of Canine Cadaver Skin‐Muscle‐Fascia Specimens

Cited by 18 publications

References 18 publications

Evaluation of information presented within soft tissue sarcoma histopathology reports in the United States: 2012‐2015

Evaluation of information presented within soft tissue sarcoma histopathology reports in the United States: 2012‐2015

Reductions in margin length after excision of grade II mast cell tumors and grade I and II soft tissue sarcomas in dogs

Summary of Skin Reconstruction Options

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