The effect of solvents polarity and extraction conditions on the microalgal lipids yield, fatty acids profile, and biodiesel properties

Zarrinmehr, Mohammad Javad; Daneshvar, Ehsan; Nigam, Subhasha; Gopinath, Kannappan Panchamoorthy; Biswas, Jayanta Kumar; Kwon, Eilhann E.; Wang, Hailong; Farhadian, Omidvar; Bhatnagar, Amit

doi:10.1016/j.biortech.2021.126303

Cited by 25 publications

(4 citation statements)

References 31 publications

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“…Hexane, petroleum ether, diethyl ether, ethanol, isopropanol, n-heptane, acetone, chloroform, methanol, and 1-butanol are some of the common organic solvents used for the extraction of volatile compounds (Miazek et al, 2017). Whereas, the polarity of the solvent plays a significant role in extracting a wide range of compounds, in which, non-polar to low-polar solvents extract a greater number of compounds than polar or high-polar solvents (Zarrinmehr et al, 2022). However, in this study, fractionation was performed by using different polar solvents ranging from low to high polarities such as hexane, dichloromethane, ethyl acetate, ethanol, and methanol, and marked as fractions F1 to F5 respectively.…”

Section: Discussionmentioning

confidence: 99%

‘Nori’ (<em>Porphyra</em> sp.), a Functional Food Showing Anticancer Activity against Ovarian Cancer

Elumalai¹,

Kanna²,

Umamaheswari³

et al. 2022

Preprint

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Marine seaweeds are nature’s largesse resource in human well-being. Seaweed’s contribution to the food industry is being widened for its rich source of essential vitamins, minerals, and PUFA. ‘Nori’ is a dried sheet of red seaweed Porphyra sp. used to make ‘sushi’ a renowned cuisine in Japan, and other Southeast Asian countries. In this present study, commercial-grade ‘Nori’ was extracted and fractionated with different solvents, and subjected to evaluate the cytotoxic activity on human ovarian tetracarcinoma cells (PA1). As a result, the ethyl acetate fraction was found effective in inhibiting the proliferation of human ovarian cancer cells with an IC50 value of 41.1 µg/mL. The main component responsible for anticancer activity was determined as a sesquiterpene lactone Tatridin B (3h-cyclodeca[b]furan-2-one, 4,9-dihydroxy-6-methyl-3,10-dimethylene-). Moreover, it was found effective in inhibiting NSD2, an important and recently proposed biomarker for several types of cancer. This is a hitherto report on the presence of this compound in ‘Nori’ showing potent anticancer activity on human ovarian tetracarcinoma (PA1).

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Section: Discussionmentioning

confidence: 99%

‘Nori’ (<em>Porphyra</em> sp.), a Functional Food Showing Anticancer Activity against Ovarian Cancer

Elumalai¹,

Kanna²,

Umamaheswari³

et al. 2022

Preprint

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“…Hence, it is necessary to explore further the extraction method and solvent used so that the bioactive contents in BSF prepupa can be optimally produced as antibacterial agents. According to Zarrinmehr et al (2022) , mixtures of solvents with higher ratio of non-polar solvents such as chloroform and hexane can be applied at higher temperatures to produce more fat and fatty acid methyl esters profile and have higher oxidative stability.…”

Section: Discussionmentioning

confidence: 99%

Methanol extract of Black soldier fly (Hermetia illucens) prepupae against Aeromonas and Staphylococcus aureus bacteria in vitro and in silico

Qosimah¹,

Santosa²,

Maftuch³

et al. 2023

Open Vet J

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Background: Staphylococcus and Aeromonas bacteria are pathogens in humans and animals. The therapy disrupts the virulence structure of the bacteria, resulting in bacterial death. Currently, chemical drugs have resulted in many resistant bacteria, so it is necessary to find alternative natural materials that are not toxic and do not quickly induce resistance. Aims: This study aimed to analyze the potential of methanol extract from BSF prepupa as an antibacterial agent against S. aureus and Aeromonas through in silico and in vitro tests. Methods: The BSF prepupae methanol extract was then analyzed for protein and fatty acid contents. Molecular docking of the active ingredients (defensin, chitin, and chitosan as well as fatty acids) in BSF was downloaded from the NCBI database and docked with the Hex Cuda 8.0 program, Correlation type parameters Shape + Electro and Grid Dimension 0.6. Docking results were analyzed using the Discovery Studio program version 21.1.1. Results: The highest fatty acid contents in the extract were palmitic acid and myristic acid. Methanol extract from BSF prepupae acted as a bactericidal agent against S. aureus at a concentration of 320 mg/ml, in contrast to Aeromonas, which still showed bacterial growth. The results of the in silico test showed that defensin–aerolysin and defensin–hemolysin were bound to the same active site area. However, the amount of binding energy produced by 69-Defensin-83-aerolysin was higher than all defensin types in BSF against Aeromonas. Chitin and chitosan showed a bond on the active site of aerolysin and hemolysin, but chitosan had a stronger bond than chitin. In silico study also showed the strongest binding affinity of BSF fatty acids to isoleucyl-tRNA synthetase of S. aureus. Conclusion: The study showed that methanol extract from BSF prepupae had more potential as an antibacterial agent against S. aureus than Aeromonas in vitro and in silico.

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“…These 62 compounds were from various classes, such as hydrocarbons, benzene derivatives, alcohols, esters, ketones, aldehydes, terpenoids, and ethers. The dominant classes of volatile organic compounds were hydrocarbons (39%) and benzene derivatives (34%), as a non-polar nhexane solvent (polarity index equivalent to 0.1) was used in the liquid-liquid extraction process (Zarrinmehr et al, 2022). Similarly, Syazana et al ( 2013) also used n-hexane as the extracting solvent for the extraction of volatile organic compounds from Tualang (Koompassia excelsa) honey.…”

Section: Characterization Of Volatile Organic Compoundsmentioning

confidence: 99%

Exploring stingless bee honey from selected regions of Peninsular Malaysia through gas chromatography–mass spectrometry–based untargeted metabolomics

Manickavasagam,

Saaid,

Lim

2024

Journal of Food Science

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Volatile organic compounds in honey are known for their considerable impact on the organoleptic properties of honey, such as aroma, flavor, taste, and texture. The type and composition of volatile organic compounds are influenced by entomological, geographical, and botanical origins; thus, these compounds have the potential to be chemical markers. Sixty‐two volatile compounds were identified using gas chromatography–mass spectrometry from 30 Heterotrigona itama (H. itama) honey samples from 3 different geographical origins. Hydrocarbons and benzene derivatives were the dominant classes of volatile organic compounds in the samples. Both clustering and discriminant analyses demonstrated a clear separation between samples from distant origins (Kedah and Perak), and the volcano plot supported it. The reliability and predictability of the partial least squares–discriminant analysis model from the discriminant analysis were validated using cross‐validation (R2: 0.93; Q2: 0.83; accuracy: 0.97) and the permutation test (p < 0.001), and the output depicted that the model is legitimate. In combination with the variable importance of projection (VIP > 1.0) and the Kruskal–Wallis test (p < 0.01), 19 volatile organic compounds (encompassed aldehydes, benzene derivatives, esters, hydrocarbons, and terpenoids) were sorted and named potent chemical markers in classifying honey samples from three geographical origins. In brief, this study illustrated that volatile organic compounds of stingless honey originated from the same bee species, but different geographical origins could be applied as chemical markers.

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The effect of solvents polarity and extraction conditions on the microalgal lipids yield, fatty acids profile, and biodiesel properties

Cited by 25 publications

References 31 publications

‘Nori’ (<em>Porphyra</em> sp.), a Functional Food Showing Anticancer Activity against Ovarian Cancer

‘Nori’ (<em>Porphyra</em> sp.), a Functional Food Showing Anticancer Activity against Ovarian Cancer

Methanol extract of Black soldier fly (Hermetia illucens) prepupae against Aeromonas and Staphylococcus aureus bacteria in vitro and in silico

Exploring stingless bee honey from selected regions of Peninsular Malaysia through gas chromatography–mass spectrometry–based untargeted metabolomics

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