The Effect of Sodium Butyrate on Adventitious Shoot Formation Varies among the Plant Species and the Explant Types

Lee, Myoung Hui; Lee, Ji-Young; Choi, Seung Hee; Jie, Eun Yee; Jeong, Jae Cheol; Kim, Cha Young; Kim, Suk Weon

doi:10.3390/ijms21228451

Cited by 9 publications

(12 citation statements)

References 61 publications

(79 reference statements)

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“…To investigate whether the enhancement of regeneration by 4PBA might be due to its HDAC inhibitor activity, in addition to its auxin activity, we performed western blot analyses to check histone acetylation levels in 4PBAtreated Arabidopsis seedlings. As previously reported in other research performed in Arabidopsis and Brassica napus (Lee et al 2020;Li et al 2014;Menge et al 2017), both TSA-treated and BUA-treated Arabidopsis seedlings exhibited increases for all types of histone acetylation we examined, i.e., H3K9Ac, H3K14Ac, H3K23Ac, H3K27Ac and H4Acs (detected H4K5Ac, K8Ac, K12Ac and K16Ac), compared to mock-treated tissues (Figure 2). In contrast, we found that histone acetylation levels of 4PBA-treated Arabidopsis seedling are indistinguishable from mock-treated tissue.…”

Section: Pba Does Not Enhance Histone H3 and H4 Acetylation At Least ...supporting

confidence: 86%

“…Indeed, both auxin treatment and wounding stress alter histone modifications, including histone acetylation, which positively regulates the expression of cellular reprogramming-related genes Yamamuro et al 2016). Trichostatin A (TSA) and butyric acid (BUA), well-known inhibitors of histone deacetylases (HDACs) that increase the level of acetylated histones, can be added during tissue culture to enhance plant tissue regeneration (Bie et al 2020;Lee et al 2020;Li et al 2014;Wójcikowska et al 2018). TSA treatment additionally alters auxin responsiveness (Li et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway

Iwase

Takebayashi

Aoi

et al. 2022

Plant Biotechnology

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4-Phenylbutyric acid (4PBA) is utilized as a drug to treat urea cycle disorders and is also being studied as a potential anticancer drug that acts via its histone deacetylase (HDAC) inhibitor activity. During a search to find small molecules that affect plant regeneration in Arabidopsis, we found that 4PBA treatment promotes this process by mimicking the effect of exogenous auxin. Specifically, plant tissue culture experiments revealed that a medium containing 4PBA enhances callus formation and subsequent shoot regeneration. Analyses with auxin-responsive or cytokinin-responsive marker lines demonstrated that 4PBA specifically enhances AUXIN RESPONSE FACTOR (ARF)-dependent auxin responses. Our western blot analyses showed that 4PBA treatment does not enhance histone acetylation in Arabidopsis, in contrast to butyric acid and trichostatin A, other chemicals often used as HDAC inhibitors, suggesting this mechanism of action does not explain the observed effect of 4PBA on regeneration. Finally, mass spectroscopic analysis and genetic approaches uncovered that 4PBA in Arabidopsis plants is converted to phenylacetic acid (PAA), a known natural auxin, in a manner independent of peroxisomal IBR3-related β-oxidation. This study demonstrates that 4PBA application promotes regeneration in explants via its auxin activity and has potential applications to not only plant tissue culture engineering but also research on the plant β-oxidation pathway.

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Section: Pba Does Not Enhance Histone H3 and H4 Acetylation At Least ...supporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway

Iwase

Takebayashi

Aoi

et al. 2022

Plant Biotechnology

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“…For example, TSA promoted the formation of callus and embryo-like structures from leaf explants of Arabidopsis [47,48]. We have already reported that the effect of sodium butyrate, one of HDAC inhibitors, on in vitro adventitious shoot formation is different for each plant species or for each type of plant tissue [49]. In this study, we reported that TSA treatment is able to increase the genome editing efficiency using lettuce and tobacco protoplasts.…”

Section: Discussionmentioning

confidence: 75%

TSA Promotes CRISPR/Cas9 Editing Efficiency and Expression of Cell Division-Related Genes from Plant Protoplasts

Choi

Lee²,

Jin

et al. 2021

IJMS

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Trichostatin A (TSA) is a representative histone deacetylase (HDAC) inhibitor that modulates epigenetic gene expression by regulation of chromatin remodeling in cells. To investigate whether the regulation of chromatin de-condensation by TSA can affect the increase in the efficiency of Cas9 protein-gRNA ribonucleoprotein (RNP) indel formation from plant cells, genome editing efficiency using lettuce and tobacco protoplasts was examined after several concentrations of TSA treatments (0, 0.1, 1 and 10 μM). RNP delivery from protoplasts was conducted by conventional polyethylene glycol (PEG) transfection protocols. Interestingly, the indel frequency of the SOC1 gene from TSA treatments was about 3.3 to 3.8 times higher than DMSO treatment in lettuce protoplasts. The TSA-mediated increase of indel frequency of the SOC1 gene in lettuce protoplasts occurred in a concentration-dependent manner, although there was not much difference. Similar to lettuce, TSA also increased the indel frequency by 1.5 to 1.8 times in a concentration-dependent manner during PDS genome editing using tobacco protoplasts. The MNase test clearly showed that chromatin accessibility with TSA treatments was higher than that of DMSO treatment. Additionally, TSA treatment significantly increased the level of histone H3 and H4 acetylation from lettuce protoplasts. The qRT-PCR analysis showed that expression of cell division-related genes (LsCYCD1-1, LsCYCD3-2, LsCYCD6-1, and LsCYCU4-1) was increased by TSA treatment. These findings could contribute to increasing the efficiency of CRISPR/Cas9-mediated genome editing. Furthermore, this could be applied for the development of useful genome-edited crops using the CRISPR/Cas9 system with plant protoplasts.

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“…To examine the relative expression level of the phytohormone-related genes as well as cell wall extension such as those encoding gibberellin (GA20ox-1), ethylene (ACO1), cytokinin (SICKX1), expansin ( EXP9 , EXP18 ), and auxin ( SIAA1 , SIAA3 ) after exposure of the plants to the VOCs, primers were designed according to the study of Tahir et al (2017) . Actin4 ( ACT4 ) was used as an internal control ( Lee et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Plant Growth Promotion by Two Volatile Organic Compounds Emitted From the Fungus Cladosporium halotolerans NGPF1

et al. 2021

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Microbial volatiles have beneficial roles in the agricultural ecological system, enhancing plant growth and inducing systemic resistance against plant pathogens without being hazardous to the environment. The interactions of plant and fungal volatiles have been extensively studied, but there is limited research specifically elucidating the effects of distinct volatile organic compounds (VOCs) on plant growth promotion. The current study was conducted to investigate the impact of VOCs from Cladosporium halotolerans NGPF1 on plant growth, and to elucidate the mechanisms for the plant growth-promoting (PGP) activity of these VOCs. The VOCs from C. halotolerans NGPF1 significantly promoted plant growth compared with the control, and this PGP activity of the VOCs was culture medium-dependent. Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography–mass spectrometry (GC–MS) identified two VOC structures with profiles that differed depending on the culture medium. The two compounds that were only produced in potato dextrose (PD) medium were identified as 2-methyl-butanal and 3-methyl-butanal, and both modulated plant growth promotion and root system development. The PGP effects of the identified synthetic compounds were analyzed individually and in blends using N. benthamiana plants. A blend of the two VOCs enhanced growth promotion and root system development compared with the individual compounds. Furthermore, real-time PCR revealed markedly increased expression of genes involved in auxin, expansin, and gibberellin biosynthesis and metabolism in plant leaves exposed to the two volatile blends, while cytokinin and ethylene expression levels were decreased or similar in comparison with the control. These findings demonstrate that naturally occurring fungal VOCs can induce plant growth promotion and provide new insights into the mechanism of PGP activity. The application of stimulatory volatiles for growth enhancement could be used in the agricultural industry to increase crop yield.

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The Effect of Sodium Butyrate on Adventitious Shoot Formation Varies among the Plant Species and the Explant Types

Cited by 9 publications

References 61 publications

4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway

4-Phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway

TSA Promotes CRISPR/Cas9 Editing Efficiency and Expression of Cell Division-Related Genes from Plant Protoplasts

Plant Growth Promotion by Two Volatile Organic Compounds Emitted From the Fungus Cladosporium halotolerans NGPF1

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