The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microalgae cultures

White, Daniel A.; Pagarette, A.; Rooks, Paul; Ali, S. Twareque

doi:10.1007/s10811-012-9849-6

Cited by 184 publications

(130 citation statements)

References 70 publications

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“…Most production scenarios accomplish this by timing medium nitrogen depletion with a predetermined cellular density, while controlling the pH of the system with CO 2 addition. However, we and others have recently shown that some algal strains accumulate TAG at a higher rate when bicarbonate is added to culturing systems [15,20]. Furthermore, TAG and starch accumulation in Chlamydomonas reinhardtii were shown to be more stable when accumulation occurred in a bicarbonate rich system [16], which offers flexibility in the harvest timing.…”

Section: Introductionmentioning

confidence: 80%

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Cellular Cycling, Carbon Utilization, and Photosynthetic Oxygen Production during Bicarbonate-Induced Triacylglycerol Accumulation in a Scenedesmus sp.

et al. 2013

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Microalgae are capable of synthesizing high levels of triacylglycerol (TAG) which can be used as precursor compounds for fuels and specialty chemicals. Algal TAG accumulation typically occurs when cellular cycling is delayed or arrested due to nutrient limitation, an environmental challenge (e.g., pH, light, temperature stress), or by chemical addition. This work is a continuation of previous studies detailing sodium bicarbonate-induced TAG accumulation in the alkaline chlorophyte Scenedesmus sp. WC-1. It was found that upon sodium bicarbonate amendment, bicarbonate is the ion responsible for TAG accumulation; a culture amendment of approximately 15 mM bicarbonate was sufficient to arrest the cellular cycle and switch the algal metabolism from high growth to a TAG accumulating state. However, the cultures were limited in dissolved inorganic carbon one day after the amendment, suggesting additional carbon supplementation was necessary. Therefore, additional abiotic and biotic experimentation was performed to evaluate in-and out-gassing of CO 2 . Cultures to which 40-50 mM of sodium bicarbonate were added consumed DIC faster than CO 2 could ingas during the light hours and total photosynthetic OPEN ACCESSEnergies 2013, 6 6061 oxygen production was elevated as compared to cultures that did not receive supplemental inorganic carbon.

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Section: Introductionmentioning

confidence: 80%

“…The use of sodium bicarbonate to enhance TAG accumulation has been demonstrated in both freshwater and marine chlorophytes as well as diatoms [15,16,20,44,45], and its use offers inherent advantages. Gaseous CO 2 is costly to store and transport, whereas bicarbonate salts can easily be transported to algal facilities and stored until needed.…”

Section: Resultsmentioning

confidence: 99%

Cellular Cycling, Carbon Utilization, and Photosynthetic Oxygen Production during Bicarbonate-Induced Triacylglycerol Accumulation in a Scenedesmus sp.

et al. 2013

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“…The cells are spherical or elliptic with a length of 35 µm and a width of 14 µm. This species is able to use both sodium bicarbonate (White et al, 2012) and carbon dioxide (de Castro Araujo and Garcia, 2005) as the carbon source for growth. The optimal temperature and pH for growth are 18-24°C and 7-9, respectively (Lavens and Sorgeloos, 1996).…”

Section: Microalgaementioning

confidence: 99%

Effects of Light Exposure and Nitrogen Source on the Production of Oil from Freshwater and Marine Water Microalgae

hattab¹,

Ghaly²

2014

American Journal of Biochemistry and Biotechnology

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Abstract:The biomass yield and oil content of Chlorella saccharophila (freshwater) and Tetraselmis suecica (marine) microalgae were investigated using various nitrogen source (ammonium nitrate, ammonium phosphate, ammonium sulfate and combination of nutrients) at various light durations (9, 16 and 24 h). NaHCO 3 was used as the carbon source. The nitrogen concentration, temperature and pH were maintained at 70 mg/L, 22°C and 8.5, respectively. The results indicated that T. suecica produced higher cell yield compared to the C. saccharophila under all levels of parameters tested. Light exposure of 24 h produced the highest biomass yield. However, the difference in cell yields between light duration of 16 and 24 h was not significant. The combination of nutrients resulted in the highest growth for both species of microalgae. However, high growth did not necessarily result in high oil yield. The oil content was much higher for C. saccharophila than T. suecica. Varying light duration had no direct effect on oil yield. The nutrient type significantly influenced the production of oil. C. saccharophila produced the highest oil yield using ammonium phosphate while T. suecica achieved the highest oil yield using ammonium nitrate. The results indicated that high algal growth does not necessarily result in high oil yield. Both, the generation of new cells and storage of oil require energy. When the cells used energy for generation of new cells they store less oil. Thus, growing C. saccharophila using the combination of nutrients at 16 h light exposure would be the optimal growth conditions for producing oil for biodiesel production.

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“…In another study, it was determined that Euthalothece ZM001 species has potential for BICCAPS system due to its biomass productivity of 1.21 g/L-day while the Spirulina species has low potential due to its low biomass productivity of 0.15-0.43 g/L-day [4]. One study found out that the used of sodium bicarbonate in culture media is advantageous because of its capability to increase microalgae cells and the amount of lipids in Tetraselmis suecica and Nannochloropsis salina [12]. However, no studies were reported in utilizing bicarbonate solutions in the growth of falcatus.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ankistrodesmus falcatus for Bicarbonate-Based Integrated Carbon Capture System (BICCAPS)

Beltran

Gravador

et al. 2018

MATEC Web Conf.

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Abstract. This study evaluates the performance of alkaliphilic microalgae Ankistrodesmus falcatus in the Bicarbonate-based Integrated Carbon Capture and Algae Production System (BICCAPS). The system utilized bicarbonate as carbon source for microalgae production. BICCAPS parameters such as pH, algal biomass productivity and CO2 utilization (inorganic carbon conversion, Ci) were observed at different sodium bicarbonate (NaHCO3) loading concentration and type of culture media. The highest productivity was observed at 10 g/L of NaHCO3 loading in BRSP medium at 3.5539 mg/L/day. This value is 30% lower compared to the control experiment (continuously aerated bioreactor). The Ci values of the different system ranges from 1.17 x 10 -4 to 1.51 x 10 -4 moles/L/day. Both the pH of the BRSP and NPK media at 10 g/L and 30g/L loading of NaHCO3 increased through time. The result shows that A. falcatus has a potential in BICCAPS utilization.

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The effect of sodium bicarbonate supplementation on growth and biochemical composition of marine microalgae cultures

Cited by 184 publications

References 70 publications

Cellular Cycling, Carbon Utilization, and Photosynthetic Oxygen Production during Bicarbonate-Induced Triacylglycerol Accumulation in a Scenedesmus sp.

Cellular Cycling, Carbon Utilization, and Photosynthetic Oxygen Production during Bicarbonate-Induced Triacylglycerol Accumulation in a Scenedesmus sp.

Effects of Light Exposure and Nitrogen Source on the Production of Oil from Freshwater and Marine Water Microalgae

Evaluation of Ankistrodesmus falcatus for Bicarbonate-Based Integrated Carbon Capture System (BICCAPS)

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