The Effect of Single Deuterium Substitutions for Protium in a DNA Molecule on the Occurrence of Open States

Deuterium-Depleted Water Influence on the Isotope 2H/1H Regulation in Body and Individual Adaptation

“…The latter effect can be caused by the influence of deuterium on the probability of occurrence of open states between complementary pairs of nitrogenous bases, which can significantly change the speed of reading genetic information. In the physiological range, the deuterium atom increases the probability for rupture the bond between the complementary nitrogenous bases by 0.22%−0.60%, which reflects its ability to slow down the reading speed of genetic information in transcription processes, narrowing down the range of regulatory mechanisms for persistent exposure during the cell cycle of the low-intensity adverse factor and leading to a decrease in the adaptive capacity of the cell [65].…”

“…Particular attention must be drawn to the possible effect of isotopic composition of the medium on the molecular dynamics of the DNA molecule which under natural conditions has one deuterium atom for every 6400 hydrogen atoms and that ratio can lead to a change in the frequency of DNA molecule mutations during living systems evolution [64]. It has been shown that singular replacements of protium atom by deuterium in hydrogen bonds between pairs of nitrogenous bases of the DNA molecule lead to change in the frequency of occurrence of its open states, which in their turn are an obligatory condition for functional activity for the molecule, including facilitating specific intermolecular DNA protein interactions during transcription, folding and replication [65]. It was found that the probability of occurrence of open states between nitrogenous bases in double-stranded DNA depends on concentration of deuterium in the liquid medium surrounding the molecule, and on the magnitude of the energy of hydrogen bonds rupture.…”

Influence of the 2H/1H Isotope Composition of the Water Environment on the Probability of Denaturation Bubble Formation in a DNA Molecule

“…These changes can be estimated only by mathematical modeling. Earlier we showed that when deuterium atoms penetrate hydrogen bonds between the nitrogenous base pairs of the DNA molecule, they may affect the probability for occurrence of open states [12]. In addition, it was shown in [13] that the probability for occurrence of open states (OSs) depends both on the deuterium concentration in the liquid medium surrounding the molecule and on the critical hydrogen bond breaking energy.…”

Influence of Single Deuterium Replacement on Frequency of Hydrogen Bond Dissociation in IFNA17 under the Highest Critical Energy Range

“…Earlier, we found that the ingress of a deuterium atom into hydrogen bonds between pairs of nitrogenous bases increases the probability of occurrence of open states by 0.22–0.60% [ 13 ]. In addition, it has been shown that the probability of the bubbles formation (length from 12 to 27 nucleotides) depends on the localization of the deuterium atom in the DNA molecule and may differ significantly from the probability of the occurrence of open states in general [ 14 ].…”