The effect of silver nanoparticles
on the reproductive system of adult male rats:
A morphological, histological and DNA integrity study

Fathi, Nasibeh; Hoseinipanah, Seyed Mohammad; Alizadeh, Zohreh; Assari, Mohammad Javad; Moghimbeigi, Abbas; Mortazavi, Motahare; Hosseini, Mahdi; Bahmanzadeh, Maryam

doi:10.17219/acem/81607

Cited by 43 publications

(26 citation statements)

References 23 publications

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“…The seminiferous tubule morphometric analysis showed that all the tubular parameters evaluated significantly reduced in the AgNPs-D7 group, whereas no difference was found between AgNPs-D14, D28, and D56 and Control groups. Similar results reported by Fathi et al (2018), showed that an intraperitoneal injection of AgNPs (125 mg/kg) caused a significant reduction in the diameter, area and circumference of seminiferous tubules. In contrast, increased diameter, area and circumference of seminiferous tubules in animals treated with AgNPs were observed by Gromadzka-Ostrowska et al (2012).…”

Section: Discussionsupporting

confidence: 88%

Acute reproductive toxicology after intratesticular injection of silver nanoparticles (AgNPs) in Wistar rats

et al. 2020

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This study aimed to evaluate the effects of an intratesticular injection of silver nanoparticles (AgNPs) on reproductive parameters and health of rats, and to evaluate the AgNPs biodistribution in order to develop a nanotechnological contraceptive agent for male animals. Treated animals received 220 lL of AgNPs solution (0.46 mg-Ag/ml) in each testicle and were euthanized: seven, 14, 28, and 56 days after injection. A significant decrease (p < 0.05) in the percentage of motile sperm in D7 (8.8%) was observed, comparing to the control (73.3%), D14 (86.0%), D28 (68.2%), and D56 (90.0%) groups. D7 group also presented a decrease (p < 0.05) in the percentage of normal spermatozoa. Additionally, D7 group showed an increase (p < 0.05) in abnormal midpiece and sperm head morphology compared to the Control group. Seminiferous tubules presented all germline cell types and spermatozoa for all groups. However, D7 group did not present spermatozoa in the epididymis, whereas some spermatozoa and cellular debris were visible in D14 and D28 groups. All animals presented hematological parameters, creatinine, and alanine aminotransferase values within the normal limits for Wistar rats. The percentage of silver found in the liver was always higher than in the other organs analyzed. A pioneering mathematical model is proposed, from which the half-life time of silver in the liver (17 days), spleen (23 days), lungs (30 days), and kidneys (35 days) was extracted. In conclusion, some acute and severe toxic effects were observed in sperm cells following intratesticular injection of AgNPs, although these effects were reversible. No adverse effects to general animal health were observed.

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Section: Discussionsupporting

confidence: 88%

Acute reproductive toxicology after intratesticular injection of silver nanoparticles (AgNPs) in Wistar rats

et al. 2020

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“…Other studies further corroborated these effects. Fathi et al () showed the noxious effects of silver nanoparticles on the sperm and seminiferous tubules, while Qin et al () stated that cerium oxide nanoparticles adversely affected testosterone levels with a concomitant depletion of mRNA expression in steroidogenic‐related genes. Furthermore, Hou and Zhu () suggested that nanoparticles could elicit various stress responses in reproductive organs via the mechanisms of oxidative stress, apoptosis and inflammation.…”

Section: Discussionmentioning

confidence: 99%

Salubrious effects of a vermiculite–cellulose‐based bionanocomposite on oxidative stress indices and histomorphology of male Wistar rats

et al. 2019

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There is a current interest from the food packaging, biomedical and agricultural sectors in hybrid materials formed from clays and natural polymeric compounds. However, research investigating the toxicity of vermiculite–cellulose nanocrystal (VERN) hybrid on the testes of Wistar rats is rare. Twenty rats, divided into control and treatment groups, were orally administered distilled water, 5, 10, and 20 mg/kg bw VERN daily for two consecutive weeks. At the termination of experiments, the testicular organo‐somatic index, superoxide dismutase, catalase, and gamma‐glutamyl transferase activities were not significantly changed by VERN relative to the controls. Contrarily, myeloperoxidase, glutathione peroxidase, and malondialdehyde levels were depleted in the testes of treated rats. Moreso, VERN increased follicle‐stimulating and luteinizing hormones, and decreased testosterone levels at the 20 mg/kg dose. Histology of the testes revealed healthy looking Leydig cells at the doses of 5 and 10 mg/kg VERN. Overall, these results indicate that oral exposure of VERN was not overly deleterious to the redox and structural histoarchitecture in the testes of rats.

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“…Further studies confirmed that the reproductive toxicities of AgNPs are achieved by increasing inflammation, disrupting DNA structure, down-regulating gene expression, decreasing mitochondrial function, inducing ROS production and apoptosis. These toxicities of AgNPs to the reproductive system are size-, time- and dose-dependent 347 , 348 . Zhang et al 347 investigated the effects of AgNPs with different sizes (10 nm and 20 nm) on male somatic Leydig cells and Sertoli cells, and found that cell viability was inhibited by AgNPs in size- and concentration-dependent manners.…”

Section: Potential Toxicity Of Agnpsmentioning

confidence: 99%

Silver nanoparticles: Synthesis, medical applications and biosafety

et al. 2020

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Silver nanoparticles (AgNPs) have been one of the most attractive nanomaterials in biomedicine due to their unique physicochemical properties. In this paper, we review the state-of-the-art advances of AgNPs in the synthesis methods, medical applications and biosafety of AgNPs. The synthesis methods of AgNPs include physical, chemical and biological routes. AgNPs are mainly used for antimicrobial and anticancer therapy, and also applied in the promotion of wound repair and bone healing, or as the vaccine adjuvant, anti-diabetic agent and biosensors. This review also summarizes the biological action mechanisms of AgNPs, which mainly involve the release of silver ions (Ag + ), generation of reactive oxygen species (ROS), destruction of membrane structure. Despite these therapeutic benefits, their biological safety problems such as potential toxicity on cells, tissue, and organs should be paid enough attention. Besides, we briefly introduce a new type of Ag particles smaller than AgNPs, silver Ångstrom (Å, 1 Å = 0.1 nm) particles (AgÅPs), which exhibit better biological activity and lower toxicity compared with AgNPs. Finally, we conclude the current challenges and point out the future development direction of AgNPs.

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The effect of silver nanoparticles on the reproductive system of adult male rats: A morphological, histological and DNA integrity study

Cited by 43 publications

References 23 publications

Acute reproductive toxicology after intratesticular injection of silver nanoparticles (AgNPs) in Wistar rats

Acute reproductive toxicology after intratesticular injection of silver nanoparticles (AgNPs) in Wistar rats

Salubrious effects of a vermiculite–cellulose‐based bionanocomposite on oxidative stress indices and histomorphology of male Wistar rats

Silver nanoparticles: Synthesis, medical applications and biosafety

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