The Effect of Silicone Tube and Silicone Tube + Hyaluronic Acid Application on Adhesion Formation in Experimental Peri- and Epi-neurorrhaphy in A Rat Model

Abstract

Following neurorrhaphy in Wistar albino rats with experimental sciatic nerve cut, the effectiveness of solely silicone tube (ST) and ST plus hyaluronic acid application on preventing fibrosis was clinically and histopathologically examined. After a total nerve cut is created in sciatic nerve, interfascicular and epineural anastomosis was used. While only anastomosis was applied for the first group (Control Group), for the second group (ST Group) anastomosis + silicone tube and for the third group (ST + HA Group) anastomosis + silicone tube + hyaluronic acid (HA) were applied. Animals in each group were divided into 2 subgroups and macroscopic and histopathological examinations were conducted on the 30th and 60th postoperative day. On day 30 of the study all the animals had problematic walks. On the 60 th day while animals in groups ST and ST + HA were walking normally, the problem was still going on for the control group. In the postmortem macroscopic examinations performed in the control group on the 30 th and 60 th days an irregular morphology and adhesion to surrounding in nerve tissue were seen. Whereas in group ST, in the anastomosis line within the tube, scar tissue which was clearer on the 60th day was observed, in group ST + HA it was seen that nerve anastomosis line was smooth on the 30th and 60th days. As a consequence, the reduction in myelin thickness and the increase in degenerated myelin for groups ST and ST + HA in 30 day show that HA does not create a positive effect on axon regeneration in the short run, on the other hand, the reduction in myelin degenerated along with the increase of axon myelin thickness and axon cross section areas in groups ST and ST + HA in day 60 has shown that silicone tube and HA application creates a positive impact on myelination in the long run. Özet Bu çalışmada deneysel siyatik sinir kesisi oluşturulan Wistar albino ratlarda nörorafiyi izleyerek, ilgili alana sadece silikon tüp (ST) ve ST ile birlikte hyaluronik asit (HA) uygulamasının fibrozisi engellemedeki etkinliği klinik ve histopatolojik olarak incelendi. Siyatik sinirde total kesi oluşturulduktan sonra interfasiküler ve epinöral anastomoz uygulandı. Birinci grupta (Kontrol Grubu) sadece anostomoz yapılırken, ikinci grubta (ST Grubu) anastomoz + silikon tüp, üçüncü grubta ise (ST + HA Grubu) anastomoz + silikon tüp + Hyaluronik asit (HA) uygulandı. Her bir gruptaki hayvanlar 2 alt gruba ayrılarak postoperatif 30 ve 60. günlerde makroskopik ve histopatolojik incelemeler yapıldı. Çalışmanın 30. gününde tüm hayvanların yürüyüşleri problemliydi. 60. günde ST ve ST + HA grubundaki hayvanlarda yürüyüş normal iken kontrol grubunda problem devam etmekteydi. Prostmortem makroskopik bakıda 30 ve 60. günlerde kontrol grubunda sinir dokuda düzensiz bir morfoloji ve çevreye yapışıklık mevcuttu. ST Grubunda 60. günde daha belirgin olmak üzere tüp içerisinde anastomoz hattında skar doku ile karşılaşılırken ST + HA grubunda 30 ve 60. günlerde sinir anastomoz hattının pürüzsüz bir görünümde olduğu saptandı. Sonuç olarak, 30 gü...

Comparision of the Glio-Protective Effects of Biopolymer Coated Electrospun Scaffolds

“…Due to their unique viscoelastic properties and good biocompatibility, unmodified and derived HAs are widely used for drug delivery, encapsulation in cell domains, and tissue regeneration [21,22]. Özaydın et al, have shown the reduction of myelin degeneration along with the increase in the axon regeneration by HA coating on silicone tubes in in vivo rat models with experimental peri and epineural neuropathy [23].…”

Nöroanastomoz Sonrası Stromal Vasküler Fraksiyonun Nörorejeneratif Etkisinin Histolojik ve Elektromiyografik Değerlendirilmesi

“…Neurorrhaphy, tissue adhesives, and grafting are the routine techniques of nerve anastomosis [1,2] . Neurorrhaphy is the most common choice, especially in situations where tension-free nerve endings are attached [1][2][3][4][5][6][7] . Fibrosis, adhesion neuroma and glioma formation after neurorrhaphy has a negative effect on nerve healing [1,4,5] .…”

“…Neurorrhaphy is the most common choice, especially in situations where tension-free nerve endings are attached [1][2][3][4][5][6][7] . Fibrosis, adhesion neuroma and glioma formation after neurorrhaphy has a negative effect on nerve healing [1,4,5] . Because of the negative effects of neurorrhaphy on healing, tissue adhesives have been developed as an alternative [1,2,8] and fibrin glue, which is used for neural anastomosis, reduces local inflammation and fibrosis and provides neurotropic factors [2,6,[9][10][11] .…”

Periferik Sinirde Kronik Konstrüksiyon Hasarı Tedavisi Üzerine Dekompresyonun Etkisi

“…carpal tunel syndrome, spinal root compression, and trigeminal neuralgia due to vascular compression [8] . Few studies have investigated the molecular mechanisms of CCI and decompression [9] whereas some studies evaluated morphological changes [10][11][12] . However experimental evidence on neuropathic pain and CCI is still lacking.…”