The Effect of Sialidase on Pig Transferrins

Abstract

1. Sialidase digestion resulted in a decreased electrophoretic mobility of pig transferrins without destroying their ability to bind iron.2. The decrease in electrophoretic mobility occurred in two distinct steps.3. The migration rates of the final sialidase digestion products of TfA and TfB suggest that the difference in mobilities of TfA and TfB is not due to a difference in the amount of sialic acid which each contains.4. The mobility differences between the three zones observed for both TfA and TfB under high voltage Tris–citrate gel analyses do not appear to be due to the amount of sialic acid possessed by each.

Difference between the Hb C variants in Brahman and in indigenous Southern African cattle breeds

“…To improve separation of Hb C and Hb F a modification of Kristjansson & Hickman's (1965) method was applied. The gel buffer used was 0.019 M Tris, 0.017 M cacodylic acid (pH 7.55) and the electrolyte buffer was 0.3 M boric acid, 0.1 M NaOH (PH 8.6).…”

“…To demonstrate the difference between the migration rates of the Hb A , Hb B, Hb C, Hb F and Hb I variants, equal quantities of blood containing Hb BZ, Hb AC and Hb F (one ml of a 10 % solution of each type) were mixed. The mixed samples were analysed by the modified Kristjansson & Hickman's (1965) method.…”

Polymorphic variation in the amount of sialic acid attached to bovine transferrin

“…A modification of the method of the Kristjansson & Hickman (1965) was used, 0.025 M Tris -HCl buffer p H 6.75 and Connaught…”

Genetic control of serum esterases in day‐old pigs1

“…After the gel halves had been stained for one hour at 37°C and the esterase patterns had been classified, the stain solution was replaced by 50% methanol and the following day the gels were wrapped in plastic film for storage. Occasionally the remaining halves of gels were stained with Amido Black 10B to reveal the position of certain serum proteins such as albumen and transferrins (Kristjansson & Cipera, 1963).…”