The effect of short‐term exposure of triamcinolone acetonide on fibroblasts and retinal pigment epithelial cells

Abstract

ABSTRACT.Purpose: We investigated the effects of short-term exposure to triamcinolone on cultured choroidal fibroblast (CFB) cells and retinal pigment epithelial (RPE) cells. Methods: To evaluate the effect of triamcinolone on cell proliferation, CFB and RPE cells were divided into three groups: a short-term exposure group; a longterm exposure group, and a non-treated control group. Cells in the shortterm exposure group were briefly exposed (5, 15 or 30 mins) to triamcinolone (0.01 mg ⁄ ml, 1 mg ⁄ ml or mitomycin C (0.01 lg ⁄ ml, 1 lg ⁄ ml). Cells in the longterm exposure group were continuously incubated in culture medium containing the drug until assessment. The control group was cultured without drugs. Cell viability and the number of cells were assessed at day 5 after exposure. To investigate the direct toxicity of triamcinolone on confluent RPE cells, completely confluent cells were exposed to the drugs in the manner as described above. Cell viability was determined on days 0, 3 and 5 after treatment. Results: In the short-term exposure group, 1 mg ⁄ ml triamcinolone caused a significant reduction in the proliferation of CFB and RPE cells. The proliferation of CFBs decreased even with exposure to 0.01 mg ⁄ ml triamcinolone. In the longterm exposure group, triamcinolone and mitomycin C reduced the proliferation of both CFB and RPE cells. Even very short periods of exposure to triamcinolone caused a significant reduction in the viability of completely confluent RPE cells. Conclusions: Even short periods of exposure to triamcinolone inhibited the proliferation of fibroblasts and RPE cells and were significantly toxic to completely confluent RPE cells.

Randomised clinical trial of intravitreal Avastin vs photodynamic therapy and intravitreal triamcinolone: long-term results

“…Furthermore, it was shown that triamcinolone may have a toxic effect on RPE cells, although its anti-inflammatory and antiangiogenetic properties may to beneficial to avoid side effects of the PDT treatments. 12,13 All of these factors may contribute to the less favourable BCVA outcomes in the combination group.…”

Microcystic Lymphatic Malformation: A Case Report

“…Triamcinolone acetonide is a long acting glucocorticoid, which not only inhibits collagen synthesis but also accelerates collagen degradation, in addition to its ability to repress inflammation [16], also glucocorticoids have hindering effects of tumor lymphangiogenesis [17]. Sclera therapy is commonly the preferred treatment method for vascular malformations [18].…”

Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells

“…Cellular uptake efficiency was investigated using fluorescein isothiocyanate labeled DA-Chit (FITC/DA-Chit) nanoparticles, instead of TAA/DA-Chit nanoparticles, by both inverted fluorescence microscopy and flow cytometry. According to the literature, 16 proliferative vitreoretinopathy can be characterized by a proliferation of retinal pigment epithelial cells. Therefore, downregulation of VEGF mRNA expression by TAA/DA-Chit nanoparticles was assessed via real-time reverse transcription polymerase chain reaction (RT-PCR) in human retinal pigment epithelial cells through the controlled release of TAA.…”

“…10 This process is usually associated with several undesirable complications, including glaucoma and endophthalmitis. 16,17 Controlled release of this corticosteroid is thus important in achieving a sustained pharmacologic effect and overcoming the drawbacks of the procedure.…”