The Effect of Shear Stress on the Fluidity of Supported Lipid Bilayers

Abstract

lived component is similar to that of 6-MI monomer, 7 ns. The position of the probe shifts the fluorescent populations from 0.4 ns to 6.5ns upon formation of duplex, which implies that 6-MI local environment in these positions resembles that of the solvent exposed monomer. However, no direct correlation between adjacent base sequence and the fluorescent properties of 6MI was observed. To further investigate the increase in fluorescence upon duplex formation, we characterized the local and global structure of several oligonucleotides through temperature melts, quantum yield calculations, quenching assays, and Raman spectroscopy. The results suggest that, the position of 6-MI in the duplex sequence, helical turn, and surrounding base sequence determines the dynamics of 6-MI. This potentially leads to the formation of a fixed geometry of 6-MI which stacks poorly with adjacent bases. The lack of stacking interactions causes 6-MI to exhibit fluorescent properties of the monomer.