The effect of shear rate on the adhesion/activation of human platelets in flow through a closed-loop polymeric tubular system

Podias, Andreas; Groth, Thomas; Missirlis, Yannis F.

doi:10.1163/156856294x00392

Cited by 16 publications

(8 citation statements)

References 18 publications

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“…Flat-plate flow chambers (Van Kruchten et al, 2012 ), parallel-plate and cone-platelet viscometer (Lackner et al, 2012 ), and tubular systems such as the “Chandler loop” (McClung et al, 2007 ; Krajewski et al, 2013 ; Stang et al, 2014 ) and the roller pump closed-loop (Podias et al, 1995 ; Wang et al, 2001 ) systems are some of in vitro shear flow models. In these models, vascular blood flow is mimicked to simulate the dynamic interaction between the biomaterial and whole blood (Sanak and Wegrzyn, 2010 ).…”

Section: Incubation Of Biomaterials With Human Bloodmentioning

confidence: 99%

Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

Weber

Steinle

Golombek

et al. 2018

Front. Bioeng. Biotechnol.

461

304

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Hemocompatibility of blood-contacting biomaterials is one of the most important criteria for their successful in vivo applicability. Thus, extensive in vitro analyses according to ISO 10993-4 are required prior to clinical applications. In this review, we summarize essential aspects regarding the evaluation of the hemocompatibility of biomaterials and the required in vitro analyses for determining the blood compatibility. Static, agitated, or shear flow models are used to perform hemocompatibility studies. Before and after the incubation of the test material with fresh human blood, hemolysis, cell counts, and the activation of platelets, leukocytes, coagulation and complement system are analyzed. Furthermore, the surface of biomaterials are evaluated concerning attachment of blood cells, adsorption of proteins, and generation of thrombus and fibrin networks.

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Section: Incubation Of Biomaterials With Human Bloodmentioning

confidence: 99%

Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

Weber

Steinle

Golombek

et al. 2018

Front. Bioeng. Biotechnol.

461

304

View full text Add to dashboard Cite

show abstract

“…1,2 He moved then to Biomaterials Research Unit at Medical Clinic Charité Berlin to work on his doctoral thesis that was focused on development of methods for testing blood compatibility of polymer membranes for hemodialysis and tubing for blood lines. [3][4][5] Thomas obtained his PhD in Biophysics at Humboldt University Berlin in 1991. During his subsequent PostDoc time at Medical School Charité Berlin, he worked on testing of blood-compatible polymers and membrane materials for novel types of hemodialyzers and other blood-contacting devices.…”

Section: Thomasmentioning

confidence: 99%

“…The topic of his thesis was on mechanism of influenza virus‐induced agglutination and fusion of human red blood cells . He moved then to Biomaterials Research Unit at Medical Clinic Charité Berlin to work on his doctoral thesis that was focused on development of methods for testing blood compatibility of polymer membranes for hemodialysis and tubing for blood lines . Thomas obtained his PhD in Biophysics at Humboldt University Berlin in 1991.…”

mentioning

confidence: 99%

Thomas Groth, PhD to serve as Co‐Editor, Europe, ESAO Representative

Malchesky¹

2020

Artificial Organs

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“…A common system used is the roller pump model (45). Roller pump models generate continuous or pulsatile flow through a peristaltic pump (52,106). Because roller pump models generate flow through compression, they can cause hemolysis, which can lead to pro-thrombotic activity unrelated to the biomaterial or flow path.…”

Section: Common Flow Loops-the Design Of In Vitro Dynamic Models To Ementioning

confidence: 99%

In Vitro models for thrombogenicity testing of blood-recirculating medical devices

Sarode

Roy

2019

Expert Review of Medical Devices

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Introduction: Blood-recirculating medical devices such as mechanical circulatory support (MCS), extracorporeal membrane oxygenators (ECMO), and hemodialyzers, are commonly used to treat or improve quality of life in patients with cardiac, pulmonary, and renal failure, respectively. As part of their regulatory approval, guidelines for thrombosis evaluation in preclinical development have been established. In vitro testing evaluates a device's potential to produce thrombosis markers in static and dynamic flow loops. Areas covered: This review focuses on in vitro static and dynamic models to assess thrombosis in blood-recirculating medical devices. A summary of key devices is followed by a review of molecular markers of contact activation. Current thrombosis testing guidance documents, ISO 10993-4, ASTM F-2888, and F-2382 will be discussed, followed by analysis of their application to in vitro testing models. Expert opinion: In general, researchers have favored in vivo models to thoroughly evaluate thrombosis, limiting in vitro evaluation to hemolysis. In vitro studies are not standardized and it is often difficult to compare studies on similar devices. As blood-recirculating devices have advanced to include wearable and implantable artificial organs, expanded guidelines standardizing in vitro testing are needed to identify the thrombotic potential without excessive use of in vivo resources during pre-clinical development.

show abstract

The effect of shear rate on the adhesion/activation of human platelets in flow through a closed-loop polymeric tubular system

Cited by 16 publications

References 18 publications

Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

Blood-Contacting Biomaterials: In Vitro Evaluation of the Hemocompatibility

Thomas Groth, PhD to serve as Co‐Editor, Europe, ESAO Representative

In Vitro models for thrombogenicity testing of blood-recirculating medical devices

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