The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose

Ingesson, Hanna; Zacchi, Guido; Yang, Bin; Esteghlalian, Ali R.; Saddler, John N.

doi:10.1016/s0168-1656(01)00273-5

Cited by 82 publications

(59 citation statements)

References 4 publications

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“…At 40% (w/w) DM, the lignin concentration was around 10% and although the ratio between enzyme and lignin was kept constant, the high-lignin concentration might affect the action of the enzymes. Mass transfer limitations has also been suggested as a cause for the lower conversion due to increased viscosity at higher solids concentrations (Ingesson et al, 2001). Based on the present results it is not possible to elucidate which mechanisms are responsible for the observed decline in enzyme performance at increasing DM.…”

Section: Effect Of Initial Dry Matter Content On Combined Liquefactiomentioning

confidence: 64%

“…Furthermore, several of these studies have only tested hydrolysis at two or three solids concentrations, thereby limiting the ability to draw conclusions on the exact relationship between solids concentration and conversion. Gradually increasing the solids concentration in the interval between 2% and 10% DM have for several substrates been demonstrated to result in a concurrent decrease in conversion in the order of 10%-20% going from 2% to 10% DM (Ingesson et al, 2001;Lu et al, 2002). However, in another study increasing the solids concentration from 2% to 5% DM resulted in a 16% decrease in cellulose conversion but a further increase to 7.5% and 10% DM resulted only in an small decrease in conversion (Tengborg et al, 2001).…”

Section: Effect Of Initial Dry Matter Content On Liquefaction and Sacmentioning

confidence: 94%

“…The correlation between stirring or shaking speed and the hydrolysis has been studied previously, but at solids concentrations below 10% and in shake flasks or stirred tank reactors. Several studies have found that although the initial hydrolysis rate is increased by increasing shaking (from 25 to 150 rpm), the final cellulose conversion are often only slightly affected by shaking speed (Ingesson et al, 2001;Mais et al, 2002a). It has also been found that intermittent stirring (periods of low or no stirring) was almost as efficient as constant stirring (Ingesson et al, 2001;Tengborg et al, 2001).…”

Section: Effect Of Mixing Speed and Chamber Fillingmentioning

confidence: 99%

“…Several studies have found that although the initial hydrolysis rate is increased by increasing shaking (from 25 to 150 rpm), the final cellulose conversion are often only slightly affected by shaking speed (Ingesson et al, 2001;Mais et al, 2002a). It has also been found that intermittent stirring (periods of low or no stirring) was almost as efficient as constant stirring (Ingesson et al, 2001;Tengborg et al, 2001). However, at increasing solids concentration the effect of stirring speed gets more pronounced (Mais et al, 2002a).…”

Section: Effect Of Mixing Speed and Chamber Fillingmentioning

confidence: 99%

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Liquefaction of lignocellulose at high‐solids concentrations

Jørgensen

Vibe-Pedersen²,

Larsen³

et al. 2006

Biotech & Bioengineering

449

323

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To improve process economics of the lignocellulose to ethanol process a reactor system for enzymatic liquefaction and saccharification at high-solids concentrations was developed. The technology is based on free fall mixing employing a horizontally placed drum with a horizontal rotating shaft mounted with paddlers for mixing. Enzymatic liquefaction and saccharification of pretreated wheat straw was tested with up to 40% (w/w) initial DM. In less than 10 h, the structure of the material was changed from intact straw particles (length 1-5 cm) into a paste/ liquid that could be pumped. Tests revealed no significant effect of mixing speed in the range 3.3-11.5 rpm on the glucose conversion after 24 h and ethanol yield after subsequent fermentation for 48 h. Low-power inputs for mixing are therefore possible. Liquefaction and saccharification for 96 h using an enzyme loading of 7 FPU/gÁDM and 40% DM resulted in a glucose concentration of 86 g/kg. Experiments conducted at 2%-40% (w/w) initial DM revealed that cellulose and hemicellulose conversion decreased almost linearly with increasing DM. Performing the experiments as simultaneous saccharification and fermentation also revealed a decrease in ethanol yield at increasing initial DM. Saccharomyces cerevisiae was capable of fermenting hydrolysates up to 40% DM. The highest ethanol concentration, 48 g/kg, was obtained using 35% (w/w) DM. Liquefaction of biomass with this reactor system unlocks the possibility of 10% (w/w) ethanol in the fermentation broth in future lignocellulose to ethanol plants.

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Section: Effect Of Initial Dry Matter Content On Combined Liquefactiomentioning

confidence: 64%

Section: Effect Of Initial Dry Matter Content On Liquefaction and Sacmentioning

confidence: 94%

Section: Effect Of Mixing Speed and Chamber Fillingmentioning

confidence: 99%

Section: Effect Of Mixing Speed and Chamber Fillingmentioning

confidence: 99%

See 2 more Smart Citations

Liquefaction of lignocellulose at high‐solids concentrations

Jørgensen

Vibe-Pedersen²,

Larsen³

et al. 2006

Biotech & Bioengineering

449

323

View full text Add to dashboard Cite

show abstract

“…At 150 rpm, the enzyme activity obtained was 0.02623 U/ml and 100 rpm gave the lowest enzyme activity with 0.02122 U/ml. Some agitation rate increases the hydrolysis rate and yield, but excessive mixing can deactivate the enzyme and reduce the conversion yield [13], [14]. The deactivation effect has been attributed to shear force generated by the mixer and the entrapment of air bubbles into the medium at the air-liquid surface.…”

Section: Effect Of Agitation Ratementioning

confidence: 99%

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Hatijah¹,

Ruhayu²

2013

IJBBB

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Abstract-Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8), the sucrose concentration (10g/l to 50 g/l) and the agitation rate (100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30 o C. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration.

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Oscillating flow bioreactors: An enabling technology for sustainable biorefining operations?

Muster-Slawitsch

Buchmaier

Brunner

et al. 2020

J Adv Manuf & Process

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In this work a novel reactor is evaluated on its potential to intensify biobased processing via a novel potentially continuous enzymatic hydrolysis process. An oscillatory flow bioreactor is applied in batch and recirculating mode, the latter mimicking the flow pattern of a continuous process. The recirculating mode operation can intensify cellulose conversion to hydrolyzed sugar liquid significantly, namely with enabling high solid processing and reaching >10% higher cellulose conversion than the respective operation in batch mode. Resulting sugar concentrations reach >90 mg/mL with the potential to enable the elimination of further concentration steps in down-stream processing, having a positive impact on the overall efficiency and sustainability of further processing steps to biofuels or biochemicals. K E Y W O R D S enzymatic hydrolysis, continuous processing, oscillatory flow

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The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose

Cited by 82 publications

References 4 publications

Liquefaction of lignocellulose at high‐solids concentrations

Liquefaction of lignocellulose at high‐solids concentrations

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Oscillating flow bioreactors: An enabling technology for sustainable biorefining operations?

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