The effect of serotonin (5-hydroxytryptamine) and derivatives on guanosine 3',5'-monophosphate in human monocytes.

Sandler, Jeffrey A.; Clyman, Ronald I.; Manganiello, Vincent C.; Vaughan, Martha

doi:10.1172/jci107948

Cited by 57 publications

(13 citation statements)

References 11 publications

(8 reference statements)

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“…In each of the three preparations examined (Table IV), the concentrations of serotonin ranged from 42 to 88 AM and were in the range previously shown to increase monocyte cGMP (16 caused accumulation of cGMP in monocytes and these fractions contained ascorbic acid (Fig. 4).…”

Section: Introductionmentioning

confidence: 58%

“…1). Dialyzable material from lysates of equivalent numbers of adherent and nonadherent mononuclear cells were equally active in increasing mononuclear cell cGMP content (Table II) (16).…”

Section: Introductionmentioning

confidence: 98%

“…Leukocyte cGMP was measured by a previously reported method (16). Briefly, leukocyte suspensions containing 107-108 cells in 2.0 ml KrebsRinger-Tris buffer were incubated for 10 min before additions of 0.4 ml of dialysate or buffer.…”

Section: Introductionmentioning

confidence: 99%

“…In each experiment cells were also exposed to 100 AM 5-hydroxytryptamine (serotonin, Sigma Chemical Co.) which causes accumulation of cGMP in monocytes (16) to confirm responsiveness of the cells.…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of monocyte cGMP by leukocyte dialysates. An antigen-independent property of dialyzable transfer factor.

Sandler¹,

Smith²,

Manganiello³

et al. 1975

J. Clin. Invest.

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A B S T R A C T We studied the effects of dialysates from leukocyte lysates containing dialyzable transfer factor activity and other leukocyte dialysates devoid of transfer factor activity on accumulation of cyclic nucleotides in human leukocytes. Dialysates from normal leukocytes produced 4-to 11-fold increases in leukocyte cGMP, and experiments with purified cell populations revealed that the increases were predominantly, if not entirely, in blood monocytes. Substances that increased monocyte cGMP could be obtained from several cell populations including mononuclear cells from HypaqueFicoll gradients, plastic-adherent monocytes, nonadherent lymphocytes, and neutrophils, but were not present in dialysates of leukemic lymphocytes from patients with the Sezary syndrome. Moreover, dialysates that increased leukocyte cGMP had essentially no effect on intracellular cAMP.Dialysates of lysed mononuclear cells contained serotonin, ascorbate, and an unidentified cholinergic activity, agents known to increase leukocyte cGMP. After passage of dialyzable transfer factor from mononuclear cells through a gel-filtration column, four fractions were obtained that increased leukocyte cGMP. Two of these fractions contained ascorbate; two other active fractions, including one that also caused conversion of delayed skin tests, did not contain detectable ascorbate or serotonin. The dialysate of lysed neutrophils also increased cGMP, but this activity was limited to the column fractions which contained ascorbic acid.These observations raise the possibility that alterations in monocyte cGMP content could modulate either the

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Section: Introductionmentioning

confidence: 58%

“…1). Dialyzable material from lysates of equivalent numbers of adherent and nonadherent mononuclear cells were equally active in increasing mononuclear cell cGMP content (Table II) (16).…”

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stimulation of monocyte cGMP by leukocyte dialysates. An antigen-independent property of dialyzable transfer factor.

Sandler¹,

Smith²,

Manganiello³

et al. 1975

J. Clin. Invest.

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“…From Table III (Illiano et al, 1973;Parker & Smith, 1973;Bourne et al, 1973;Parker et al, 1974;Sandler et al, 1975;Goldberg & Haddox, 1977;Atkinson et al, 1977;Polgar et al, 1977;Takemoto et al, 1978). However, only a few data have been reported on cyclic nucleotide patterns in purified preparations of human T and B lymphocytes.…”

Section: Methodsmentioning

confidence: 99%

Patterns of cyclic nucleotides in normal and leukaemic human leucocytes

Peracchi¹,

Maiolo²,

Lombardi³

et al. 1980

Br J Cancer

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Summary.-Because recent observations indicate that metabolism of cyclic nucleotides may be altered in neoplastic cells, the intracellular levels of cyclic adenosine 3',5'-monophosphate (cAMP) and cyclic guanosine 3',5'-monophosphate (cGMP) were measured in mononuclear leukaemic and normal human leucocytes. The activities of adenylate cyclase, guanylate cyclase and cyclic nucleotide phosphodiesterases were also determined. Under basal conditions, cAMP levels were always higher in the normal leucocytes, whilst cGMP levels were of the same order of magnitude in both normal and leukaemic cells, causing the cAMP/cGMP ratios to be significantly lower in leukaemic leucocytes. Leukaemic cells significantly increased cyclic nucleotide levels in response to theophylline, but did not respond to serotonin, carbamylcholine or D,L-isoproterenol. Preincubation of these leucocytes with theophylline produced a detectable cAMP response to D,L-isoproterenol but no cGMP response to serotonin or carbamylcholine was found. Adenylate cyclase and guanylate cyclase were significantly lower in leukaemic than in normal cells, which could largely explain the abnormal cyclic nucleotide pattern found in human leukaemic leucocytes. In our experiments, cAMP phosphodiesterase activity was comparable in normal and leukaemic cells, whereas cGMP phosphodiesterase activity was undetectable in all mononuclear-leucocyte preparations with the methods used.

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Metabolism of Cyclic GMP in Vascular Smooth Muscle, Leukocytes, and Lung

Vaughan

1976

Eukaryotic Cell Function and Growth

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The effect of serotonin (5-hydroxytryptamine) and derivatives on guanosine 3',5'-monophosphate in human monocytes.

Cited by 57 publications

References 11 publications

Stimulation of monocyte cGMP by leukocyte dialysates. An antigen-independent property of dialyzable transfer factor.

Stimulation of monocyte cGMP by leukocyte dialysates. An antigen-independent property of dialyzable transfer factor.

Patterns of cyclic nucleotides in normal and leukaemic human leucocytes

Metabolism of Cyclic GMP in Vascular Smooth Muscle, Leukocytes, and Lung

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