The Effect of Sericin with Variable Amino-Acid Content from Different Silk Strains on the Production of Collagen and Nitric Oxide

Aramwit, Pornanong; Kanokpanont, Sorada; De-Eknamkul, Wanchai; Kamei, Kaeko; Srichana, Teerapol

doi:10.1163/156856209x453006

Cited by 109 publications

(85 citation statements)

References 36 publications

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“…It has been reported that sericin can induce collagen production 23,24) and promote fibroblast proliferation without the activation of pro-inflammatory cytokines, 23,25) and Aramwit and Sangcakul 32) showed that the topical application of sericin enhances wound healing rate and produces a woundsize reduction in rats. We also reported that sericin has a potent effect in promoting the corneal wound healing rate in rats, probably by increasing cell movement and proliferation in corneal epithelial cells.…”

Section: )mentioning

confidence: 99%

“…19) Besides acting as a natural moisturizing agent, sericin also possesses anticoagulating, antioxidant, antiwrinkle, and antibacterial activities, and enhances the proliferation of mammalian cells in serum-free media. [20][21][22] In addition, sericin can induce collagen production 23,24) and promote fibroblast proliferation 23) without the activation of pro-inflammatory cytokines. 25) In this study, we undertook kinetic analyses of skin wound healing in normal rats and diabetic model rats, streptozotocininduced diabetic rats (STZ rat).…”

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confidence: 99%

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Combination Ointment Containing Solid Tranilast Nanoparticles and Dissolved Sericin Is Efficacious for Treating Skin Wound-Healing Deficits and Redness in Diabetic Rats

Nagai

Ogata

Deguchi

et al. 2017

Biological & Pharmaceutical Bulletin

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We attempted to design a combination ointment containing solid tranilast nanoparticles and dissolved sericin as a wound-healing drug (TS-combination ointment), and evaluated its usefulness as therapy for wound-healing deficits in streptozotocin-induced diabetic rat (STZ rat) using kinetic analyses as an index. Solid tranilast nanoparticles were prepared by bead mill methods with low-substituted methylcellulose; the mean particle size of the tranilast nanoparticles was 70 nm. The ointment was designed to contain the tranilast nanoparticles plus sericin powder and/or Carbopol ® 934. Skin wound healing in STZ rats begins significantly later than in normal rats. Although the skin wound healing rate in STZ rats treated with an ointment containing tranilast nanoparticles was lower than in STZ rats treated with vehicle, the ointment was effective in reducing redness. An ointment containing sericin enhanced the skin-healing rate, but the preventive effect on redness was weak. On the other hand, the combination of tranilast and sericin increased both the skin healing rate and reduction in redness. In conclusion, we have adapted kinetic analyses to skin wound healing in rats, and found these analyses to be useful as an index of wound healing ability by a wound-healing drug. In addition, we show that treatment with the TS-combination ointment enhances the skin wound healing rate and reduces redness. These findings provide information significant to the search for new wound-healing therapies and for the design of wound-healing drugs.Key words tranilast; sericin; skin wound healing; redness; diabetes mellitus Diabetes mellitus is characterized by a dysfunction in glucose metabolism associated with the subsequent negative effects on lipid and protein metabolism. The most common dermatological complications in diabetic patients are woundhealing deficits.1) Wound healing is a preprogrammed process consisting of four continuous phases: (1) bleeding controlled by hemostasis, (2) inflammatory response, (3) cellular and collagen proliferation, and (4) remodeling.2-5) Following injury, the inflammatory response takes place from 0-3 d, and cellular proliferation occurs at 3-12 d. During the inflammatory response, monocytes and macrophages release various cytokines, and the number of fibroblasts increases. The fibroblasts induce collagen synthesis, and collagen replenishes the skin deficit area. Thus, collagen synthesis via cytokine release (inflammatory response) is important in the process of skin wound healing. On the other hand, wound healing via abnormal collagen synthesis and formulation during a prolonged inflammatory response is the cause of redness and keloid formation in the skin. A decrease in collagen synthesis results in delayed skin healing.

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Section: )mentioning

confidence: 99%

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confidence: 99%

Combination Ointment Containing Solid Tranilast Nanoparticles and Dissolved Sericin Is Efficacious for Treating Skin Wound-Healing Deficits and Redness in Diabetic Rats

Nagai

Ogata

Deguchi

et al. 2017

Biological & Pharmaceutical Bulletin

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“…Sericin is non-toxic to fibroblast cells. Methionine and cysteine content in silk sericin are important factors to promote cell growth and collagen synthesis [91]. Water-soluble silk sericin has no immunogenicity and is also a biocompatible macromolecular protein like silk fibroin [92].…”

Section: Sericinmentioning

confidence: 99%

Biopolymeric Nanoparticles

2017

Design of Nanostructures

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This review on nanoparticles highlights the various biopolymers (proteins and polysaccharides) which have recently revolutionized the world of biocompatible and degradable natural biological materials. The methods of their fabrication, including emulsification, desolvation, coacervation and electrospray drying are described. The characterization of different parameters for a given nanoparticle, such as particle size, surface charge, morphology, stability, structure, cellular uptake, cytotoxicity, drug loading and drug release, is outlined together with the relevant measurement techniques. Applications in the fields of medicine and biotechnology are discussed along with a promising future scope.

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“…Sericin was indicated to suppress lipid peroxidation and tyrosinase inhibitory activity (Kato et al, 1998). In addition to antioxidant activity and chemopreventive effects of sericin was found (Fan et al, 2009;Zhaorigetu et al, 2003), Silk sericin from silkworm cocoons accelerates the proliferation of mammalian cell line (Terada et al, 2002;Aramwit et al, 2009;Aramwit and Sangcakul 2007). Another study reported anti-cancer effects of sericin in colon cancer (Kaewkom et al, 2012).…”

Section: Cytotoxicity Assay Of Cocoon Extractsmentioning

confidence: 99%

Cytocompatibility of silkworm cocoon layer extracts

Jo¹,

Kim²,

Lee³

et al. 2016

International Journal of Industrial Entomology

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Recently silk polymer produced by Bombyx mori silkworm has been considered as biological macromolecules. Silk polymer was extracted in PBS solution at 37 o C for 72 h or 72 o C for 24 h. The effect of EtOH treatment on the cocoon extraction was also examined. The extraction yield of cocoon was less than 1 wt% regardless of extraction conditions. UV spectroscopy showed that the experimental extracts have absorption bands at 280 nm. There is no cytotoxicity effect on the mouse fibroblast L929 cell. The phenotype of L929 cell was not changed under the experimental conditions. The proliferation behavior of L929 cell was not affected by the addition of cocoon extract. Therefore, cocoon extract might be cytocompatible and can be used as promising biomaterials. IntroductionA silkworm cocoon of Bombyx mori provides silk fiber for textile industries for more than 4,000 years. Recently, silk polymer has been revealed several advantages for biological materials and then applied various functional materials such as cosmetics, suture and artificial eardrum (Kundu et al., 2013;Ju et al., 2014;Vepari and Kaplan, 2007; Kim et al., 2010), and so on.In the view point of material, silk is a natural protein polymer composed of two proteins: one is a central protein called silk fibroin and another is gum-like protein called silk sericin. Silk fibroin is a natural fibrous protein with a semicrystalline structure and it consists of heavy chain (390 kDa), light chain (25 kDa) and glycoprotein. Each polypeptides linked by disulfide bond (Zhou et al., 2001).Especially, fibroin as the main component of silk protein has found diverse applications in the biomedical field, which has a strong tensile strength, biodegradability and biocompatibility (Horan et al., 2005;Meinel et al., 2005). Hence, many researchers select silk fibroin as one of the materials for biomedical application.Silk sericin is a protein polymer, which acts as an adhesive binder to maintain the structure of the fibers. Sericin is an albuminoid protein composed of serine (30%) and other amino acids (Teramoto and Miyazawa, 2005;Garel et al., 1997). Sericin was removed easily from Bombyx mori cocoon using a thermo or chemical process called degumming (Mo et al., 2009) Preparation of cocoon extractsEach cocoon was washed with tap water and then dried absolutely with room temperature in the dark. The cocoon was sliced into 5 mm ⅹ 5 mm pieces. Each cocoon slices were divided into two groups; one group was treated with absolute ethyl alcohol (EtOH) for 24 h at R.T. and another group was not treated EtOH. After treatment, cocoon was dried with R.T. for overnight. The extraction condition were followed; weigh 1 g of cocoon and add into 10 mL of 1X PBS buffer at 37 o C for 72 h and/or 72 o C for 24 h. Save the cocoon extracts and then filtered it with 0.22 um syringe filter (Sartorius Ltd., Epsom, UK) and protein concentration of all extracts was determined by bicinchronic acid (BCA) assay. Forty ug of each extracts used for cell cytotoxicity and proliferation assay. UV vis sp...

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The Effect of Sericin with Variable Amino-Acid Content from Different Silk Strains on the Production of Collagen and Nitric Oxide

Cited by 109 publications

References 36 publications

Combination Ointment Containing Solid Tranilast Nanoparticles and Dissolved Sericin Is Efficacious for Treating Skin Wound-Healing Deficits and Redness in Diabetic Rats

Combination Ointment Containing Solid Tranilast Nanoparticles and Dissolved Sericin Is Efficacious for Treating Skin Wound-Healing Deficits and Redness in Diabetic Rats

Biopolymeric Nanoparticles

Cytocompatibility of silkworm cocoon layer extracts

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