The Effect of Semen Dilution on Morphology and Fertility of Japanese Quail (<I>Coturnix coturnix japonica</I>) Spermatozoa

Abstract

The e#ect of quail semen dilution with three extenders : Lake's, Ringer's and Tyrod's on morphology and fertility trials of unstored spermatozoa was evaluated.Semen was collected twice a week by male stimulation by female method, from 0* quail males randomly divided into four groups (I-IV). Forty-eight females divided into four respective groups (A-D) were inseminated intravaginally with +* ml of fresh or ,* ml of ,-folds diluted semen.Semen dilution did not a#ect the number of live spermatozoa in total, but the number of live, morphologically normal spermatozoa depended on the diluent. The highest number of morphologically normal spermatozoa ῍0/.-῍ vs. 01./῍ in fresh semen was stated in semen diluted with Lake's extender. It was significantly higher (Pῌ*.*+) than in semen diluted with Ringer's diluent (/0.,῍).Egg fertility (33.,῍), hatchability from set (3+.,῍) and fertile eggs (3-.3῍) in naturally mated group were significantly higher (Pῌ*.*+) when comparing with females inseminated artificially both, with fresh and diluted semen. The highest fertility (0*.+῍) was obtained for spermatozoa diluted with Lake's extender, the lowest for spermatozoa treated with Ringer's solution (-*.,῍).

Effect of freeze‐dried quail egg white and yolk addition to semen extender on viability of rooster sperm stored for 6 h at 4°C

“…The effect of semen extenders on morphology and sperm motility in semen stored for 6 h at 4°C described in the presented experiment is consistent with our previous, and other authors’ observations in drake (Łukaszewicz, Jerysz, & Chełmońska, 2020), roosters (Dumpala et al, 2006; Łukaszewicz, Jerysz, & Kowalczyk, 2020) and quail semen (Chełmońska et al, 2006). The range of changes depended on the level of lyophilized egg white or yolk in the extender.…”

“…Semen dilution is a very important initial step of in vitro processing, storage and preservation for efficient artificial insemination (Smith et al, 2018). Numerous studies indicate a significant influence of diluents and storage time on sperm morphology and motility parameters in chicken (Donoghue & Wishart, 2000; Dumpala et al, 2006; Hudson et al, 2016; Iaffaldano et al, 2008; Łukaszewicz, Jerysz, & Kowalczyk, 2020; Mphaphathi et al, 2016; Santiago‐Moreno et al, 2012; Siudzińska & Łukaszewicz, 2008; Vasicek et al, 2015), turkey (Di Iorio et al, 2020; Słowińska et al, 2018), guinea fowl (Hudson et al, 2016; Keerthy et al, 2016), ostrich (Smith et al, 2018), capercaillie (Kowalczyk et al, 2012), quail (Chełmońska et al, 2006), duck (Łukaszewicz, Jerysz, & Chełmońska, 2020) and goose (Łukaszewicz, 2002a, 2002b). Semen preservation is also a very important tool necessary in rescue and conservation of bird species and breeds.…”

Evaluating sperm quality characteristics obtained through the female teaser method in native chicken breeds

“…Physiologically, sexual stimulation leads to rapid erection and ejaculation within a few seconds, whereas the massage method typically requires around one minute of stimulation [5]. However, it is essential to emphasize that the success of both semen collection methods depends on the selection of suitable males and their acclimation to the procedure.…”

“…Several methods have been developed for semen collection in poultry, including massage methods, with one of the cooperative approaches being the use of a stimulation dummy or female teaser [4]. The semen collection method significantly impacts sperm quality and subsequent egg fertility [5].…”

“…Another semen collection method in poultry is the female teaser approach, which has been employed in quails, ostriches, emu birds, and native chickens [5], with the successful production of high-quality sperm. However, the semen collection method has not been extensively used in native chicken breeding units as an alternative to support AI technology.…”