The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on endoplasmic reticulum stress and apoptosis in chicken spleen induced by heat stress

Xu, Danning; Li, Bingxin; Tian, Yunbo; Huang, Yunmao

doi:10.1039/c6ra27730f

Cited by 21 publications

(12 citation statements)

References 36 publications

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“…For example, many studies in broilers showed that the dosage of Se, added at a range of 0.3-1.0 mg/kg to the diet, improved the growth performance of chicken and quails when exposed to heat stress, contributing to the improvement of immune function and the increased activities of glutathione, GSH-Px, and SOD (47)(48)(49)(50)(51)(52)(53). It is also implied that a greater amount of Se is required for enhancing selenium-dependent antioxidant enzyme activities and gene expression relative to the glutathione system to alleviate oxidative damage induced by heat stress in poultry (54)(55)(56)(57)(58)(59). According to the positive result of antioxidant ability, we will further investigate the Se requirement as well as the effect of Se yeast supplementation in diets in duck layers under heat-stressed condition in our next study.…”

Section: Discussionmentioning

confidence: 99%

Effects of Selenium-Enriched Yeast on Performance, Egg Quality, Antioxidant Balance, and Egg Selenium Content in Laying Ducks

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Effects of Selenium-Enriched Yeast on Performance, Egg Quality, Antioxidant Balance, and Egg Selenium Content in Laying Ducks

et al. 2020

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“…It suggested that PAMK can relieve the HS-induced oxidative stress in the chicken spleen. Furthermore, previous studies also stated that PAMK may antagonize apoptosis in the chicken [ 36 , 40 ]. In the present study, PAMK decreased the expressions of pro-apoptotic genes, and increased the expression of anti-apoptotic gene.…”

Section: Discussionmentioning

confidence: 99%

The protective effects of polysaccharide of Atractylodes macrocephala Koidz (PAMK) on the chicken spleen under heat stress via antagonizing apoptosis and restoring the immune function

Cao

et al. 2017

Oncotarget

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Heat stress can cause immune organ dysfunction and apoptosis. Polysaccharide of Atractylodes macrocephala Koidz may have protective effects on immune organs. In this study, we established chicken models of Polysaccharide of Atractylodes macrocephala Koidz-heat stress interaction and detected the oxidative index, activities of mitochondrial complexes and ATPases as well as the ultrastructure in chicken spleens. Expression levels of cytokines, mitochondrial dynamics- and apoptosis-related genes were also measured. In the result, heat stress increased the expression of interleukin 1 and tumour necrosis factor-alpha and decreased that of interleukin 2 and interferon gamma. The activities of mitochondrial complexes and ATPases were decreased and oxidative stress was induced by heat stress. Besides, expressions of the mitochondrial dynamics- and anti-apoptosis-related genes were decreased and those of pro-apoptosis-related genes were increased by heat stress. HS induced pathological changes of mitochondria and triggered apoptosis in chicken spleens. However, these adverse effects triggered by HS were remarkably alleviated in Polysaccharide of Atractylodes macrocephala Koidz + heat stress group. This study confirmed the protective effects of Polysaccharide of Atractylodes macrocephala Koidz on the chicken spleen against the heat stress and revealed its mechanism, which is that Polysaccharide of Atractylodes macrocephala Koidz could relieve the heat stress-induced immune dysfunction of chicken spleens via reducing oxidative stress, enhancing the mitochondria function and inhibiting apoptosis.

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“…A previous study showed that GRP78 expression was increased by the pyrrolidine dithiocarbamate/Cu complex [21]. The expression GRP78 was significantly elevated in the spleen of chicken induced by heat stress [22]. In pig, the CHOP protein content was increased following exposure to the high temperature [7].…”

Section: Discussionmentioning

confidence: 99%

Effects of cage and floor rearing system on the factors of antioxidant defense and inflammatory injury in laying ducks

Zhang

Tian

et al. 2019

BMC Genet

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BackgroundCage-rearing in laying ducks, as a novel rearing system, not only fundamentally solves the pollution problem of the duck industry and improve bio-safety and product quality but also exhibits more benefits by implementing standardized production compared with the floor-rearing. Of course, this system also brings some welfare problems and stress injuries to layers due to lack of water environment and limited activities in the cages. However, the effects on the factors of antioxidant defense and inflammatory injury in the early cage stage are not well-understood.ResultsIn this study, eighty Shaoxing layers were reared on floor and in cages from 12 weeks of age. The ducks were caged 1, 2, 4, 7, and 10 days, the factors of antioxidant defense and inflammatory injury were investigated. The results showed that the caged ducks suffered liver injury to a certain extent when the ducks were just put into the cages. Analysis of antioxidant enzyme activities indicated that the different rearing system could not affect the change of antioxidant capacities, while the liver malondialdehyde (MDA) level was significant higher in the 2-d, 7-d, and 10-d ducks compared with the 1-d ducks during the change of days, while catalase (CAT) activity showed the opposite results. Additionally, quantitative real-time PCR (qRT-RCR) revealed that the relative mRNA levels of endoplasmic reticulum (ER) stress-related gene (CHOP and GRP78) were significantly upregulated in cage rearing ducks compared to that of the floor rearing ducks. Moreover, the mRNA levels of inflammatory cytokines including cycloxygenase-2 (COX-2), nitric oxide synthase (iNOS), Interleukin 1 beta (IL-1β), Interleukin 2 (IL-2) and Interleukin 6 (IL-6), were also increased significantly in caged layers.ConclusionsTaken together, although antioxidant defense has no obvious effect on cage stress, the stress levels of laying ducks vary greatly in the early cage stage, which not only caused liver tissue damage to some extent, but also resulted in increases in the expression of the factors of inflammatory injury. Therefore, we recommend that anti-stress agents should be added in the feed to alleviate the stress in the early cage stage.

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The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on endoplasmic reticulum stress and apoptosis in chicken spleen induced by heat stress

Abstract: Endoplasmic reticulum (ER) stress and oxidative stress are involved in different types of stress induced injuries.

Cited by 21 publications

References 36 publications

Effects of Selenium-Enriched Yeast on Performance, Egg Quality, Antioxidant Balance, and Egg Selenium Content in Laying Ducks

Effects of Selenium-Enriched Yeast on Performance, Egg Quality, Antioxidant Balance, and Egg Selenium Content in Laying Ducks

The protective effects of polysaccharide of Atractylodes macrocephala Koidz (PAMK) on the chicken spleen under heat stress via antagonizing apoptosis and restoring the immune function

Effects of cage and floor rearing system on the factors of antioxidant defense and inflammatory injury in laying ducks

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