The effect of selected pre‐analytical phase variables on plasma thromboxane <scp>A</scp><sub>2</sub> measurements in humans

Sadílková, Lenka; Paluch, Zoltán; Bednář, František; Alušík, Š

doi:10.1111/j.1751-553x.2012.01458.x

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Accordingly, it is essential that blood collection, processing, and handling methods are reported in detail when providing oxylipin data. For example, higher serum values of TXB 2 relative to plasma are attributed to platelet activation [46][47][48], and use of heparin in plasma generation is postulated to stimulate lipoprotein lipases, which results in concomitant increases in overall oxylipin levels [44].…”

Section: Oxylipin Composition Of Serum and Plasma Evidences Similar Pmentioning

confidence: 99%

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega‐3 fatty acids

Lundström

Yang

Brannan

et al. 2013

Molecular Nutrition Food Res

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Scope In contrast to well-characterized polyunsaturated fatty acid (PUFA) levels in serum, little is known regarding their downstream metabolic products. However, many of these compounds are lipid mediators with prominent roles during pro- and anti-inflammatory processes. Methods and Results In this double blind crossover study on asthmatics, shifts in serum levels of ω-3 and ω-6 PUFA-derived oxidized fatty acids (e.g., eicosanoids, oxylipins) were quantified following dietary fish oil supplementation. Serum was obtained from subjects following fasting at 3 occasions; 1) Prior to supplementation, 2) Following a 3-week supplement intake of either placebo or fish oil, and 3) Following a 3-week washout period with a subsequent 3-week period of either fish oil or placebo supplement. A total of 87 oxylipins representing cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened via liquid chromatography-tandem mass spectrometry (LC-MS/MS). The primary alterations observed were in CYP- and 15-LOX-derived EPA- and CYP-derived DHA oxylipins. Conclusion The results indicate that intake of an ω-3 rich diet altars not only the PUFA ratio, but also the ratio of downstream oxylipins. These data further support that dietary manipulation with ω-3 PUFAs affects not only PUFA levels, but importantly also the downstream metabolic profile.

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Section: Oxylipin Composition Of Serum and Plasma Evidences Similar Pmentioning

confidence: 99%

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega‐3 fatty acids

Lundström

Yang

Brannan

et al. 2013

Molecular Nutrition Food Res

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Influence of pre-analytical time and temperature conditions on serum thromboxane B 2 levels

Diemen

Fuijkschot

Spit

et al. 2018

Thrombosis Research

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Suppressive effect of citalopram on plasma concentrations of thromboxane B₂

Richter¹,

Alušík

Paluch

et al. 2015

Scandinavian Journal of Clinical and Laboratory Investigation

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The effect of selected pre‐analytical phase variables on plasma thromboxane A₂ measurements in humans

Abstract: This study describes the setup of such pre-analytical phase conditions that enable the screening of platelet function in terms of the plasma concentrations of TxB₂ and 11-dTxB₂ in selected EIA measurement.

Cited by 3 publications

References 23 publications

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega‐3 fatty acids

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega‐3 fatty acids

Influence of pre-analytical time and temperature conditions on serum thromboxane B 2 levels

Suppressive effect of citalopram on plasma concentrations of thromboxane B₂

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The effect of selected pre‐analytical phase variables on plasma thromboxane A2 measurements in humans

Abstract: This study describes the setup of such pre-analytical phase conditions that enable the screening of platelet function in terms of the plasma concentrations of TxB₂ and 11-dTxB₂ in selected EIA measurement.

Cited by 3 publications

References 23 publications

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega‐3 fatty acids

Lipid mediator serum profiles in asthmatics significantly shift following dietary supplementation with omega‐3 fatty acids

Influence of pre-analytical time and temperature conditions on serum thromboxane B 2 levels

Suppressive effect of citalopram on plasma concentrations of thromboxane B2

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Product

Resources

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The effect of selected pre‐analytical phase variables on plasma thromboxane A₂ measurements in humans

Suppressive effect of citalopram on plasma concentrations of thromboxane B₂