Studies designed to examine high-frequency seafloor scattering or penetration mechanisms have been limited by the difficulty of accurately characterizing seabed properties at millimetre scales. Both the two-dimensional (2D) interface controlling roughness scattering and the three-dimensional (3D) internal structure of the top tens of centimetres of seafloor sediments controlling volume scattering require accurate measurement tools that are non-destructive and that have high spatial resolution. Subcritical penetration mechanisms have not been satisfactorily explained to date due in part to the lack of two-dimensional seafloor roughness measurements. Recently, tools for addressing these experimental shortfalls have been developed to support seafloor acoustics measurements, and information obtained from these systems is being used to address many open questions in high-frequency seafloor acoustics. This paper provides a review with examples of the development and use of several non-traditional technologies in seafloor acoustics studies including x-ray computed tomography and digital photogrammetry.