The effect of sample treatment on separation profiles of tear fluid proteins: Qualitative and semi-quantitative protein determination by an automated analysis system

Schmut, O.; Horwath‐Winter, Jutta; Zenker, Andrea; Trummer, Gabriele

doi:10.1007/s00417-002-0537-0

Cited by 31 publications

(17 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The size is most often measured by SGE [36][37][38][39][40][41]; however, other techniques have been increasing lately, such as CE-SDS [25,42] and ME-SDS systems [43,44]. The protein pI is another important physicochemical property that is normally measured by IEF either in the slab-or strip-gel format [24,36,40,45,46].…”

Section: General Characterizationmentioning

confidence: 99%

Electrophoresis of pharmaceutical proteins: Status quo

Little

Paquette²,

Roos

2006

Electrophoresis

View full text Add to dashboard Cite

The biotechnology industry has undergone rapid growth in recent years largely due to the development and success of protein-based therapeutics for a wide range of disorders. Similar to traditional pharmaceuticals, characterization of a therapeutic protein for its physicochemical properties, process monitoring and lot release is crucial. Electrophoresis in the slab-gel format has and continues to be a mainstay of the protein laboratory; and more recently, CE has begun to make significant inroads for protein analysis in industrial settings. This review focuses on the electrophoresis of proteins with an emphasis on protein-based therapeutics in the capillary, slab-gel and to a lesser extent, the microchip format. Reported applications of electrophoresis at several stages of the biopharmaceutical industry covering the period of 2000-2005 will be discussed.

show abstract

Section: General Characterizationmentioning

confidence: 99%

Electrophoresis of pharmaceutical proteins: Status quo

Little

Paquette²,

Roos

2006

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…The Bioanalyzer in combination with the Protein LabChip kit have been employed to analyze a variety of protein samples such as cell lysates [81], proteins present in body fluids, tear fluids [82], serum proteins [83], antibodies [84], proteins extracted from tissues [85], and from venoms [86]. A description of these applications may be found in the last section of the present review.…”

Section: Cge On Chipsmentioning

confidence: 99%

Recent innovations in protein separation on microchips by electrophoretic methods

Peng¹,

Pallandre²,

Tran³

et al. 2007

Electrophoresis

View full text Add to dashboard Cite

Microchips for analytical purposes have attracted great attention over the last 20 years. In the present review, we focus on the most recent development of microchips for electrophoretic separation of proteins. This review starts with a short recalling about the microchips covering the basic microchip layout for CE and the commercial chips and microchip platforms. A short paragraph is dedicated to the surface treatment of microchips, which is of paramount importance in protein analysis. One section is dedicated to on-line sample pretreatment in microchips and summarizes different strategies to pre-concentrate or to purify proteins from complex matrixes. Most of the common modes used for CE of proteins have already been adapted to the chip format, while multidimensional approaches are still in progress. The different routes to achieve detection in microchip are also presented with a special attention to derivatization or labeling of proteins. Finally, several recent applications are mentioned. They highlight the great potential of electrophoretic separations of proteins in numerous fields such as biological, pharmaceutical or agricultural and food analysis. A bibliography with 151 references is provided covering papers published from 2000 to the early 2007.

show abstract

“…The minimal invasiveness of the methods of sample collection makes tears a very interesting option for proteomic studies, not only in ocular diseases, but also in other conditions, e.g., diabetes mellitus [19][20][21][22] or cancer [23]. Tear film proteins were extensively analyzed by HPLC [24,25], 2-DE [26,27] and other chromatographic approaches [28]. Attempts have been made to establish a tear film protein map [14,29].…”

Section: Tear Filmmentioning

confidence: 99%

Proteomics in ocular fluids

Grus

Joachim

Pfeiffer

2007

Proteomics Clinical Apps

View full text Add to dashboard Cite

The focus of this article is to review recent techniques in proteomic analysis of ocular fluids. These fluids include tears, aqueous humor, and vitreous, they will also be compared to serum analysis. Furthermore, we attempt to summarize some disease correlated biomarkers in ocular fluids that were discovered through different proteomic techniques in eye diseases like dry eye, glaucoma, age-related macular degeneration, uveitis, or diabetic retinopathy. This review is trying to point out the importance of these biomarkers for clinical applications.

show abstract

The effect of sample treatment on separation profiles of tear fluid proteins: Qualitative and semi-quantitative protein determination by an automated analysis system

Cited by 31 publications

References 25 publications

Electrophoresis of pharmaceutical proteins: Status quo

Electrophoresis of pharmaceutical proteins: Status quo

Recent innovations in protein separation on microchips by electrophoretic methods

Proteomics in ocular fluids

Contact Info

Product

Resources

About