The Effect of Saliva Officinalis L (Sage Tea) on Biochemical and Pathological Changes Improvment in Adult Male Rats Induced by a High Fat Diet

Abstract

In order to investigate the physiological-protective effect of sage tea (Salvia officinalis L.) through its role in lipid profile regulation in male laboratory rats, current research has been carried out. In this study, sixty albino male laboratory rats (100-160 g) and older rats (4-7 weeks) were used. Based on the experimental nature of the study, animals were randomly divided into three main groups (six rats per group), a control group was considered to be the first group to be fed on a standard diet, and a 28-day atherogenic diet group was considered to be the second group to be fed on a standard diet (A). And the other group (B) (including subgroups) were previously fed 28 days on an atherogenic diet; oral administration was conducted as follows: the third group (B) included subgroups (B1) and (B2) administered 14 and 28 days of sage tea (S. officinalis) (0.086 mg/kg body weight) respectively. The measurement of changes in body weight and liver function enzymes (ALT, AST and ALP) and liver organ histopathology trials was done.In the results of the study, a significant increase (p ≤ 0.05) in the body weight of rats fed a high-fat diet (HFD) (group A) was seen compared to the control group fed a standard diet. Although body weights in The other groups were reduced in the sage tea treated group ( group B), the findings of the test of liver function enzymes in animals fed on an atherogenic diet (group A) revealed a substantial increase (p ≤ 0.05) in the level of the ALT enzyme associated with the non-changeable rate of the enzymes (AST) and (ALP), although they revealed a mild elevation relative to the control group. a slight decrease in all serum liver enzyme levels without significant difference in all experimental treatments (groups B) for both periods of 14 and 28 days. In relation to group (A), the increase and stabilization of liver enzyme levels at normal levels relative to control group values was reported, especially in the long term (28 days). In addition, histopathological examinations in the present sample showed histological changes in the liver organ compared with the control group due to feeding on a high fat diet (group A). These changes include blood vessels congestion, fat accumulation of liver connective tissue. In animals treated with sage tea (group A), a microscopic analysis of the liver showed typical histological characteristics with less fat deposition and minimal changes in histo-structural characteristics relative to the atherogenic diet group of animals. This indicates that improvement has been associated with long-term therapy (28 days). In conclusion, in reducing the functional performance and normal histological characteristics of long-term treatment-related liver and kidney organs, sage tea administration has an important synergistic protective role (28 days).