The Effect of Salicylates on the Precipitation of Antigen With Antibody

Abstract

1. Sodium salicylate modifies the precipitation of normal rabbit serum protein by sodium tungstate, and partially inhibits the precipitation of horse serum euglobulin by rabbit antiserum. Sodium salicylate added to a system containing crystalline egg albumin and its antibody partly prevents the formation of precipitate, the degree of inhibition being related to the concentration of salicylate. 2. Precipitation in the equivalence zone is more readily prevented by salicylate than precipitation in the region of antibody excess, the immune system becoming progressively less sensitive to the action of salicylate as the excess of antibody becomes larger. 3. Formed precipitates were partly dissolved following resuspension in the presence of salicylate. 4. The salicylate effect on immune precipitation is reversible, and appears to be due to inactivation of antibody. 5. Salicylate was more effective in preventing specific precipitation than other anions of a lyotropic series tested.

Effects of salicylates on blood changes in mycoplasma arthritis in rats

“…When added in vitro, even high concentrations of salicylate did not reduce the action of these antibodies on M. arthritidis growth. The interaction of metabolic inhibition antibody with M. arthritidis antigen appears to differ in this respect from the precipitation reaction of egg albumen or of type III pneumococcus polysaccharide with rabbit antiserum; these reactions are potently inhibited by salicylate (Coburn & Kapp, 1943;Friend, 1953;Austen, 1963).…”

Inhibition of the Secondary Antibody Response in Vitro by Salicylate and Gentisate

“…In comparing studies on salicylate inhibition in vivo with our investigations in vitro we shall consider initially (a) the significance of total and non-protein-1 This discussion of experimental results must be prefaced by assurance that salicylate does not interfere with the assay procedure itself, since the drug has been reported to reduce specific precipitation in at least one immune system. For example, Coburu and Kapp (19) added 1.32 mE sodium salicylate to a system of crystalline egg albumin and its antibody and observed that the precipitate was reduced by an amount equivalent to eliminating about half of the total antibody. In an identical system Friend (20) found that 2.0 n~ sodium salicylate caused a 54% reduction in the precipitation.…”

Immunochemical Properties of Native and Denatured Horse Serum Globulins

“…In neither instance was the effect of salts directed toward an increased solubility of the precipitate since no additional precipitation occurred upon dialysis unless more antigen was added. This is in contrast to sodium salicylate which appears merely to increase the solubility, by way of its action on the antibody (37). While ultracentrifugal analysis revealed the heavy antibody component to be split under the influence of NaCNS, yielding a remarkably homogeneous solution with a sedimentation constant approximating that of normal globulin, it is unlikely that this is the factor responsible for inhibition of precipitation.…”