The effect of Roundup on the bivalve Unio tumidus mollusk utilizing ex vivo approach

Abstract

dingly to a decrease of the MT and/or GSH concentrations. However, the levels of TBARS and PC were not changed comparing to control detecting the early stage of the injury. Surprisingly, NRR increased in the exposures to higher concentrations of glyphosate, probably due to strong chelating ability of glyphosate or other compounds of formulation. This study allows us to detect the earlier biological effects of glyphosate in the low environmentally realistic concentrations. Further validation of this approach needs the comparison of the results in the ex vivo and in vivo experiments.

Комбінована Дія Низької Концентрації Раундапу На Двостулкового Молюска Активує Тіолові Сполуки У Травній Залозі

“…The chromatographic properties of MTs were also non-disturbed in the exposure to 80 nM of Rn alone and in combinations [10]. Only in the acute ex vivo exposure, the similar to utilized here about 40 nM concentration of glyphosate (Rn) caused the decrease of MTSH concentration (by ~ two times) in coordination with the depletion of total antioxidant activity [10,11,12]. However, the level of TBARS was not changed compared to control detecting the early stage of the injury.…”

“…1 and [10, 11]). When we related the effects induced by the concentration of Rn alone with the two times lower Rn concentration used in combined exposures, the decreased SOD but increased levels of GSH, MTSH and LPO were indicated [10][11][12]. Consequently, despite different responses of SOD, in the exposures to Rn, the same main role of MTSH was shown.…”

“…For metallothioneins detection, the combined samples from five specimens (total 350 mg) were prepared in triplicate. The methodology used for each biomarker is given in detail in Khoma et al (2020) [10,12].…”

“…In our previous studies, we reported high sensitivity of freshwater mussels to the local environmental peculiarities (even depending on the location of the power plant dam) [8,11]. In the subchronic exposure in vivo to Roundup alone (80 nM of glyphosate) and in combinations with pharmaceuticals and heating, particular toxicity of Roundup to the mussels has been shown [10,12]. Importantly, ex vivo exposure to Roundup at as low as 40 nM glyphosate concentration caused the most prominent responses of stress and toxicity [12].…”

“…In the subchronic exposure in vivo to Roundup alone (80 nM of glyphosate) and in combinations with pharmaceuticals and heating, particular toxicity of Roundup to the mussels has been shown [10,12]. Importantly, ex vivo exposure to Roundup at as low as 40 nM glyphosate concentration caused the most prominent responses of stress and toxicity [12]. This concentration corresponds to approximately a half of the Predicted No Effect Concentration (PNEC) estimate derived from multispecies data [25].…”