The effect of Roundup on embryonic development, early foxr1 and hsp70 gene expression and hatching of common carp (Cyprinus carpio L.)

Socha, Magdalena; Szczygieł, Joanna; Brzuska, E; Sokolowska-Mikolajczyk, M.; Stonawski, Bartłomiej; Grzesiak, Małgorzata

doi:10.1016/j.theriogenology.2021.09.007

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…It has been shown that enhanced Hsp70 could protect skeletal muscle cells against oxidative stress (Hernández-Santana et al, 2014). The fertilized eggs of carp exposed to environmentally relevant concentrations of the Roundup formulation, exhibited disrupted embryonic development and alterations in foxr1 and Hsp70 gene transcripts (Socha et al, 2021). Hsp70 inhibited ERK and c-Jun N-terminal kinase (JNK) phosphorylation in mouse embryonic fibroblasts (Lee et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Alterations in cell viability, reactive oxygen species production, and modulation of gene expression involved in mitogen–activated protein kinase/extracellular regulating kinase signaling pathway by glyphosate and its commercial formulation in hepatocellular carcinoma cells

2023

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Glyphosate (N-phosphonomethyl glycine) is a non-selective, organophosphate herbicide widely used in agriculture and forestry. We investigated the possible toxic effects of the glyphosate active compound and its commercial formulation (Roundup Star®) in the human hepatocellular carcinoma (HepG2) cell line, including their effects on the cytotoxicity, cell proliferation, reactive oxygen species (ROS) levels, and expression of oxidative stress-related genes such as HO-1, Hsp70 Nrf2, L-FABP, and Keap1. MTT and NRU tests indicated that the IC50 values of Roundup Star® were 219 and 140 μM, respectively, and because glyphosate failed to induce cell death at the studied concentrations, an IC50 value could not be determined for this cell line. Roundup Star at concentrations of 50 and 100 μM significantly increased (39.58% and 52%, respectively) cell proliferation, which 200 μM of glyphosate increased by 35.38%. ROS levels increased by 27.97% and 44.77% for 25 and 100 μM of Roundup Star and 32.74% and 38.63% for 100 and 200 μM of glyphosate exposure. In conclusion, Roundup Star and glyphosate significantly increased expression levels of selected genes related to the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathway. This suggests that ROS production and the MAPK/ERK signaling pathway may be key molecular mechanisms in the toxicity of glyphosate in liver cells.

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Section: Discussionmentioning

confidence: 99%

Alterations in cell viability, reactive oxygen species production, and modulation of gene expression involved in mitogen–activated protein kinase/extracellular regulating kinase signaling pathway by glyphosate and its commercial formulation in hepatocellular carcinoma cells

2023

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Effect of cryopreservation on motility, DNA integrity and gene expression in grey mullet, Mugil cephalus sperm

Balamurugan,

Karthik,

Arul

2024

Cryobiology

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Roundup in the Reproduction of Crucian Carp (Carassius carassius): An In Vitro Effect on the Pituitary Gland and Ovary

Socha

Szczygieł

Chyb

et al. 2022

Animals

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Roundup, the most popular herbicide in global agriculture, is regarded as an endocrine disruptor causing alterations of important hormones at the hypothalamic−pituitary−gonadal axis as well as impairment of gametogenesis. The whole pituitary glands of crucian carp (Carassius carassius) were incubated for 3 h in the medium containing Roundup (0-control, 1 and 10 ng/mL). The level of luteinizing hormone (LH), and mRNA transcript abundance of kisspeptin (kiss-1) and its receptor (gpr54), were determined. The isolated ovarian fragments were incubated for 24 h in the presence of Roundup and the following effects on reproductive parameters were determined: the final oocyte maturation and ovulation, structural changes in follicles, secretion of 17,20β-progesterone (17,20β-P) as well as mRNA transcript abundance of the luteinizing hormone receptor (lhr), estrogen receptors (erα, erβ1, erβ2), and zona radiata (chorion) proteins (zp2 and zp3). Roundup inhibited final oocyte maturation and decreased the percentage of ovulated eggs, and furthermore, caused structural changes in the ovarian follicular components. There were no significant changes in the measured hormone levels and analyzed genes mRNA transcript abundance. Summing up, obtained results indicate that Roundup may adversely affect oocyte maturation and the quality of eggs, suggesting that exposure to this herbicide can lead to reproductive disorders in fish.

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The effect of Roundup on embryonic development, early foxr1 and hsp70 gene expression and hatching of common carp (Cyprinus carpio L.)

Cited by 3 publications

References 61 publications

Alterations in cell viability, reactive oxygen species production, and modulation of gene expression involved in mitogen–activated protein kinase/extracellular regulating kinase signaling pathway by glyphosate and its commercial formulation in hepatocellular carcinoma cells

Alterations in cell viability, reactive oxygen species production, and modulation of gene expression involved in mitogen–activated protein kinase/extracellular regulating kinase signaling pathway by glyphosate and its commercial formulation in hepatocellular carcinoma cells

Effect of cryopreservation on motility, DNA integrity and gene expression in grey mullet, Mugil cephalus sperm

Roundup in the Reproduction of Crucian Carp (Carassius carassius): An In Vitro Effect on the Pituitary Gland and Ovary

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