The Effect of Rotating Magnetic Field on Enterotoxin Genes Expression in Staphylococcus Aureus Strains

Abstract

Staphylococcus aureus cultures exposed to rotating magnetic field (RMF) were studied in order to analyse the possible induced changes in staphylococcal enterotoxin genes (se) expression. Liquid cultures of S. aureus strains carrying different se were exposed to the RMF of magnetic frequency 50 Hz and magnetic induction 34 mT for 10 h at 37 °C. Three time points of bacterial growth cycle were considered for RNA extractions. Gene expression analyses were evaluated using real-time quantitative PCR method. The present study confirmed, that the RMF can stimulate the growth rate of S. aureus cultures in comparison to the unexposed controls, while the stimulation is not strain dependent. The studies have also shown, that the RMF, depending on the exposure time but regardless the bacterial strain, can influence on the expression of various se. In general, except for sea, as a result of bacterial exposure to the RMF through subsequent growth phases, the expression of se decreased, reaching the values below results recorded for unexposed controls. In the case of sea expression remained at a lower level as compared to the control, regardless the time of exposition.

Regulatory and Enterotoxin Gene Expression and Enterotoxins Production in Staphylococcus aureus FRI913 Cultures Exposed to a Rotating Magnetic Field and trans-Anethole

“…The results of basic microbiological studies presented in this article were an extension of the previous studies in this area presented in the cited reference works [ 34 , 35 , 37 , 38 , 39 , 40 ]. The cited experimental studies were focused on the influence of the interaction between RMF and antibiotics, as well as the analysis of bacterial colony growth dynamics, the metabolic activity of cells, as well as genetic activity of selected bacterial species exposed to RMF.…”

“…Moreover, Giorgi et al [ 49 ] observed that mini-Tn10 and Tn5 transposition decreased under sinusoidal MF and increased under pulsed-square MF. Finally, Fijałkowski et al [ 35 ] reported a lower expression level of sea , seb , sec , sed , and see genes under RMF influence compared to non-exposure samples. Interestingly, these authors observed lower expressions of the sea gene compared to our data.…”

“…The idea of gene expression, investigated in S. aureus under RMF and TA influence, originated from previous research performed by Fijałkowski et al [ 35 ] and Qiu et al [ 36 ]. The studies showed that RMF [ 35 ] and fennel essential oil (FEO), which contains a high level of TA [ 36 ], decreased the expression of genes encoding selected SEs.…”

“…The idea of gene expression, investigated in S. aureus under RMF and TA influence, originated from previous research performed by Fijałkowski et al [ 35 ] and Qiu et al [ 36 ]. The studies showed that RMF [ 35 ] and fennel essential oil (FEO), which contains a high level of TA [ 36 ], decreased the expression of genes encoding selected SEs. However, the studies on the influence of simultaneous exposure of S. aureus to RMF and TA at the same time on changes in SEs genes expression have not been published so far.…”

Study of the Effects of Audible Sounds and Magnetic Fields on Staphylococcus aureus Methicillin Resistance and mecA Gene Expression

“…They also shown that the treatment affected the growth rate of the microbe. 29,30 Our point of view regarding the variation in gene expression of the genes under study is mainly due to strain variation, as different strains of S. aureus might react differently to the treatments under study and thus led to the variations. On the other hand, the effect of each treatment on the up or down regulation of the target genes is unclear, as no study has explored such type of treatments on those genes.…”