The effect of roselle leaf (Hibiscus sabdariffa L.) extract gel on wound healing

Abstract

Roselle (Hibiscus sabdariffa L.) belongs to the genus Hibiscus with proven anti-inflammatory, antioxidant, and antimicrobial properties. Scientific evidence associated roselle content with bioactive compounds, such as phenolic acids, flavonoids, and anthocyanin. Most studies focused on their petals, while research on leaf extract on wound healing has never been done. This study aimed to assess the effect of roselle leaf extract on wound healing in rats. This was an experimental laboratory study with a posttest-only control group design. There were 30 rats divided into 5 groups: negative control, 5%, 10%, and 15% roselle leaf extract, and positive control (bioplacenton). The parameters assessed in this research were wound size and histological assessment. The data were analyzed using ANOVA. P<0.05 was considered statistically significant. Wound healing percentage and epithelial thickness in the 15% group were the largest (84.17%; 64.69 µm). The lowest value was recorded in placebo (64%; 36.33 µm). Meanwhile, wound healing percentage and epithelial thickness of rats in the 5% and 10% groups were 68.53%, 43,57 µm, and 78.11%, 56.49 µm, respectively. Finally, positive control had a 77.44% wound healing percentage and 49.7 µm epithelial thickness. There were no significant differences in wound healing and epithelial thickness among the groups. Roselle leaf extract at 15% concentration showed greater wound healing properties based on clinical and histological assessment. Although there were no statistically significant differences, roselle leaf showed an opportunity to be further investigated as a potential wound healing therapy.

Analysis of Interleukin-6 and Tumour Necrosis Factor-Alpha Expression After Therapy of Roselle Flower Extract (Hibiscus Sabdariffa L) on the Incision Wound Lips Wistar Rat

“…Monocyte cells express TNFα in their cytoplasm and surrounding their brownish membrane. 13 Based on the results of the ANOVA different test (table 2 and figure 1), it was found that TNF Alpha was not significantly different, with a p-value of 0.054. This could be because the tissue staining failed, leading to false positive results, or because different colors were used to prepare the histological images.…”

“…Rosella flower extract 15% 13 in paste preparation made by preparing dried roselle flower obtained from Temon District, Kulon Progo Regency, Yogyakarta, as much as 500 grams. The process of drying roselle flower using an oven at 40ºC.…”

“…Preparations that have been put into a container, then labeled and stored in the refrigerator. 13,14 They are making incisions using scalpel and blade no 11. The incision wound was made on the upper labial mucosa of the rat with a depth of 1 mm and a wound width of 5 mm.…”

“…12 Rosella extract 15% was able to provide good results in the wound healing process. 13 Until now research on the effect of rosella flowers on IL-6 dan TNF Alpha expressions in the inflammatory phase has not been carried out, so it is necessary to know the effect of Roselle flower (Hibiscus sabdarifa L) 15% on the expression of IL-6 and TNF Alpha in incision wounds of Wistar white rats in the inflammatory stage of the wound healing process, especially on lips mucosa.…”

Therapeutic Potential of Hibiscus sabdariffa Linn. in Attenuating Cardiovascular Risk Factors

“…Frequently reported flavonoids that are found abundantly are quercetin-3-glucoside, methyl epigallocatechin, myricetin, quercetin, rutin, and kaempferol [ 26 ]. Other types of flavonoids present in roselle include sabdaritrin, hibiscitrin, gossytrin, gossypitrin, luteolin, and pelargonidic acid [ 27 , 28 ]. In some studies, phenolic acid was noted to be present in roselle calyx, stems, and leaves.…”