The effect of riverine dissolved organic matter and other nitrogen forms on the growth and physiology of the dinoflagellate Prorocentrum minimum (Pavillard) Schiller

Ou, Linjian; Lundgren, Veronica; Lü, Songhui; Granéli, Edna

doi:10.1016/j.seares.2013.08.005

Cited by 29 publications

(7 citation statements)

References 47 publications

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“…Specific growth rate (μ, day -1 ) was calculated using the following equation [ 28 ]: μ = (ln N2 – ln N1)/(t2 – t1), where N 1 and N 2 were the cell densities at the time t 1 and t 2 .…”

Section: Methodsmentioning

confidence: 99%

Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana: Comparative proteomics

et al. 2017

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Diatoms are important components of marine ecosystems and contribute greatly to the world’s primary production. Despite their important roles in ecosystems, the molecular basis of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was coupled with a series of physiological and biochemical techniques to explore oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses. A total of 1151 proteins were detected; 122 and 56 were significantly differentially expressed from control under N- and P-limited conditions, respectively. In N-limited cells, responsive proteins were related to reactive oxygen species (ROS) accumulation, oxidative stress responses and cell death, corresponding to a significant decrease in photosynthetic efficiency, marked intracellular ROS accumulation, and caspase-mediated programmed cell death activation. None of these responses were identified in P-limited cells; however, a significant up-regulation of alkaline phosphatase proteins was observed, which could be the major contributor for P-limited cells to cope with ambient P deficiency. These findings demonstrate that fundamentally different metabolic responses and cellular regulations are employed by the diatom in response to different nutrient stresses and to keep the cells viable.

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Section: Methodsmentioning

confidence: 99%

Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana: Comparative proteomics

et al. 2017

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“…Three 1 mL aliquots of each bottle were collected every day and fixed in 2% Lugol’s solution for subsequent cell counting under a light microscope ( Zhang et al, 2014 ). The specific growth rate of S. costatum was calculated using the following equation: μ = (ln N 2 – ln N 1 )/(t 2 – t 1 ), where N 1 and N 2 were the cell densities at time t 1 and t 2 ( Ou et al, 2014 ). Three 5 mL aliquots of each bottle collected at 11:00 am each day were dark acclimated for 15 min and the Fv/Fm was measured using PHYTO-PAM (Pulse Amplitude Modulation, ED, Walz, Effeltrich, Germany; Zhang et al, 2007 ).…”

Section: Methodsmentioning

confidence: 99%

Comparative Transcriptomic Analysis Reveals Novel Insights into the Adaptive Response of Skeletonema costatum to Changing Ambient Phosphorus

et al. 2016

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Phosphorus (P) is a limiting macronutrient for diatom growth and productivity in the ocean. Much effort has been devoted to the physiological response of marine diatoms to ambient P change, however, the whole-genome molecular mechanisms are poorly understood. Here, we utilized RNA-Seq to compare the global gene expression patterns of a marine diatom Skeletonema costatum grown in inorganic P-replete, P-deficient, and inorganic- and organic-P resupplied conditions. In total 34,942 unique genes were assembled and 20.8% of them altered significantly in abundance under different P conditions. Genes encoding key enzymes/proteins involved in P utilization, nucleotide metabolism, photosynthesis, glycolysis, and cell cycle regulation were significantly up-regulated in P-deficient cells. Genes participating in circadian rhythm regulation, such as circadian clock associated 1, were also up-regulated in P-deficient cells. The response of S. costatum to ambient P deficiency shows several similarities to the well-described responses of other marine diatom species, but also has its unique features. S. costatum has evolved the ability to re-program its circadian clock and intracellular biological processes in response to ambient P deficiency. This study provides new insights into the adaptive mechanisms to ambient P deficiency in marine diatoms.

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“…A subgroup of phytoplankton (primarily phytoflagellates, mixotrophic flagellates, and cyanobacteria) that benefit from the mixotrophic potential that DOM provides, populates DOM-rich waters. Their ability to use this alternate form of energy will enable these cells to outcompete strict autotrophs in waters with low concentrations of inorganic nutrients (Beamud, Karrasch, & Diaz, 2014;Flynn et al, 2013;Glibert & Legrand, 2006;Gran eli, Carlsson, & Legrand, 1999;Monchamp, Pick, Beisner, & Maranger, 2014;Ou, Lundgren, Lu, & Gran eli, 2014), or to use alternative sources of N such as DON to satiate the N needs of the otherwise autotrophic cells. DON-containing organic materials as simple as urea have been recently implicated as an alternative source of N linked to an increase in cyanobacteria blooms over the past decade (O'Neil et al, 2012).…”

Section: Exposure To Cyanobacteria Toxinsmentioning

confidence: 99%

Global change‐driven effects on dissolved organic matter composition: Implications for food webs of northern lakes

Creed

Bergström

Trick

et al. 2018

Global Change Biology

261

213

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Northern ecosystems are experiencing some of the most dramatic impacts of global change on Earth. Rising temperatures, hydrological intensification, changes in atmospheric acid deposition and associated acidification recovery, and changes in vegetative cover are resulting in fundamental changes in terrestrial-aquatic biogeochemical linkages. The effects of global change are readily observed in alterations in the supply of dissolved organic matter (DOM)-the messenger between terrestrial and lake ecosystems-with potentially profound effects on the structure and function of lakes. Northern terrestrial ecosystems contain substantial stores of organic matter and filter or funnel DOM, affecting the timing and magnitude of DOM delivery to surface waters. This terrestrial DOM is processed in streams, rivers, and lakes, ultimately shifting its composition, stoichiometry, and bioavailability. Here, we explore the potential consequences of these global change-driven effects for lake food webs at northern latitudes. Notably, we provide evidence that increased allochthonous DOM supply to lakes is overwhelming increased autochthonous DOM supply that potentially results from earlier ice-out and a longer growing season. Furthermore, we assess the potential implications of this shift for the nutritional quality of autotrophs in terms of their stoichiometry, fatty acid composition, toxin production, and methylmercury concentration, and therefore, contaminant transfer through the food web. We conclude that global change in northern regions leads not only to reduced primary productivity but also to nutritionally poorer lake food webs, with discernible consequences for the trophic web to fish and humans.

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The effect of riverine dissolved organic matter and other nitrogen forms on the growth and physiology of the dinoflagellate Prorocentrum minimum (Pavillard) Schiller

Cited by 29 publications

References 47 publications

Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana: Comparative proteomics

Differential cellular responses associated with oxidative stress and cell fate decision under nitrate and phosphate limitations in Thalassiosira pseudonana: Comparative proteomics

Comparative Transcriptomic Analysis Reveals Novel Insights into the Adaptive Response of Skeletonema costatum to Changing Ambient Phosphorus

Global change‐driven effects on dissolved organic matter composition: Implications for food webs of northern lakes

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