Dynamic oscillation rheometry in combination with repeating freeze-thaw (FT) cycles (DOR-FT) was applied to evaluate the rheological change of starch gel caused by long-term retrogradation. The gels prepared from corn, wheat, rice, potato, and sweet potato starch pastes (6%, w/w) were formed on a dynamic rheometer plate and the rheological changes induced by the FT cycles were measured while their gels remained on the rheometer plate. Comparing with the storage modulus (G 0 ) at 25°C of each starch gels without FT, G 0 of corn, wheat, rice, potato, and sweet potato starch gels after three FT cycles increased by 341, 474, 167, 1368, and 631%, respectively. The DOR-FT method enabled the evaluation of the rheological changes caused by the long-term retrogradation within 1 h, which is much shorter than with the conventional puncture test. Moreover, G 0 versus FT cycles could be expressed as an exponential function model, indicating that the function model would show the FT cycle dependence for the rheological change of starch gels.