The Effect of Resveratrol on Protecting Corneal Epithelial Cells from Cytotoxicity Caused by Moxifloxacin and Benzalkonium Chloride

Tsai, Tzu-Yun; Chen, Ta-Ching; Wang, I‐Jong; Yeh, Chao‐Yuan; Su, Ming‐Jai; Chen, Ruey-Hua; Tsai, Tsong-Chang; Hu, Fung‐Rong

doi:10.1167/iovs.14-15708

Cited by 32 publications

(23 citation statements)

References 56 publications

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“…Recently, mounting evidence has demonstrated that RSV may serve as a promising therapeutic reagent in a variety of acute and chronic neurodegenerative diseases [17,20,31,32,33]. It has been proven that RSV has various pharmacological functions such as anti-inflammatory, anti-oxidant, anti-apoptotic, anti-bacterial, and anti-cancer properties [20,32,34,35,36]. Among these beneficial roles, the anti-inflammatory property is significant in the neuroprotective effect of RSV.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Attenuates Acute Inflammatory Injury in Experimental Subarachnoid Hemorrhage in Rats via Inhibition of TLR4 Pathway

Zhang

et al. 2016

IJMS

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Toll-like receptor 4 (TLR4) has been proven to play a critical role in neuroinflammation and to represent an important therapeutic target following subarachnoid hemorrhage (SAH). Resveratrol (RSV), a natural occurring polyphenolic compound, has a powerful anti-inflammatory property. However, the underlying molecular mechanisms of RSV in protecting against early brain injury (EBI) after SAH remain obscure. The purpose of this study was to investigate the effects of RSV on the TLR4-related inflammatory signaling pathway and EBI in rats after SAH. A prechiasmatic cistern SAH model was used in our experiment. The expressions of TLR4, high-mobility group box 1 (HMGB1), myeloid differentiation factor 88 (MyD88), and nuclear factor-κB (NF-κB) were evaluated by Western blot and immunohistochemistry. The expressions of Iba-1 and pro-inflammatory cytokines in brain cortex were determined by Western blot, immunofluorescence staining, or enzyme-linked immunosorbent assay. Neural apoptosis, brain edema, and neurological function were further evaluated to investigate the development of EBI. We found that post-SAH treatment with RSV could markedly inhibit the expressions of TLR4, HMGB1, MyD88, and NF-κB. Meanwhile, RSV significantly reduced microglia activation, as well as inflammatory cytokines leading to the amelioration of neural apoptosis, brain edema, and neurological behavior impairment at 24 h after SAH. However, RSV treatment failed to alleviate brain edema and neurological deficits at 72 h after SAH. These results indicated that RSV treatment could alleviate EBI after SAH, at least in part, via inhibition of TLR4-mediated inflammatory signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Resveratrol Attenuates Acute Inflammatory Injury in Experimental Subarachnoid Hemorrhage in Rats via Inhibition of TLR4 Pathway

Zhang

et al. 2016

IJMS

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“…[66] Cells exhibited ∼100% viability after incubation with F-Cip 1 and 2 , and Cip with concentrations up to 10 μM compared to cells without any treatment. Benzalkonium chloride is known to be cytotoxic [74-76] and killed ∼100% cells and served as control. There was no significant difference among Cip, 1 and 2 , indicating that modification of Cip with a fluorous tag did not change its cytotoxicity.…”

Section: Resultsmentioning

confidence: 99%

Development of ciprofloxacin-loaded contact lenses using fluorous chemistry

et al. 2017

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In this work, we developed a simple method to load drugs into commercially available contact lenses utilizing fluorous chemistry. We demonstrated this method using model compounds including fluorous-tagged fluorescein and antibiotic ciprofloxacin. We showed that fluorous interactions facilitated the loading of model molecules into fluorocarbon-containing contact lenses, and that the release profiles exhibited sustained release. Contact lenses loaded with fluorous-tagged ciprofloxacin exhibited antimicrobial activity against Pseudomonas aeruginosa in vitro, while no cytotoxicity towards human corneal epithelial cells was observed. To mimic the tear turnover, we designed a porcine eye infection model under flow conditions. Significantly, the modified lenses also exhibited antimicrobial efficacy against Pseudomonas aeruginosa in the ex vivo infection model. Overall, utilizing fluorous chemistry, we can construct a drug delivery system that exhibits high drug loading capacity, sustained drug release, and robust biological activity.

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“…By contrast, others revealed that treatment with resveratrol, a natural SIRT1 activator, restored VEGF secretion in RPE cells upon oxidative and inflammatory conditions [48]. Recent literature reported the anti-aging effect of resveratrol in cutaneous, endothelial and corneal wound healing [49][50][51]. In addition to antioxidant and anti-inflammatory properties, resveratrol stimulates SIRT1 activity through allosteric interaction and increases SIRT1 affinity for both NAD+ and the acetylated substrate [28,29].…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Restores LINE-1 Methylation Levels by Modulating SIRT1 and DNMTs Functions in Cellular Models of Age-Related Macular Degeneration

Maugeri¹,

Barchitta²,

Mazzone³

et al. 2018

Preprint

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The role of epigenetic alterations in the pathogenesis of age-related macular degeneration (AMD) has been pending so far. Our study investigated the effect of oxidative stress and inflammation on DNA methyltransferases (DNMTs) and Sirtuin 1 (SIRT1) functions, as well as on long interspersed nuclear element-1 (LINE-1) methylation, in human retinal pigment epithelial (ARPE-19) cells. Therefore, we evaluated whether treatment with resveratrol may restore changes in LINE-1 methylation by modulating DNMTs and SIRT1 functions. Cells were treated with 25 mU/ml glucose oxidase (GOx) or 10 µg/ml lipopolysaccharide (LPS) to mimic oxidative or inflammatory conditions, respectively. Oxidative stress decreased DNMT1, DNMT3a, DNMT3b and SIRT1 expression (p-values <0.05), as well as total DNMTs (-28.5%; p<0.0001) and SIRT1 (-29.0%;p<0.0001) activities. Similarly, inflammatory condition decreased DNMT1 and SIRT1 expression (p-values<0.05), as well as total DNMTs (-14.9%;p=0.007) and SIRT1 (-20.1%;p<0.002) activities. Interestingly, GOx- and LPS-treated cells exhibited lower LINE-1 methylation compared to controls (p-values<0.0001). We also demonstrated that treatment with 10 μM resveratrol for 24 hours counteracted the detrimental effect on LINE-1 methylation via increasing DNMTs and SIRT1 functions in cells upon oxidative and inflammatory conditions. However, further studies should explore the perspectives of resveratrol as a suitable strategy for the prevention and/or treatment of AMD.

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The Effect of Resveratrol on Protecting Corneal Epithelial Cells from Cytotoxicity Caused by Moxifloxacin and Benzalkonium Chloride

Abstract: The protective effects of antioxidant agents indicate that MOX, similar to BAC, causes oxidative stress-related cell damage. The results also inspired us to think about a "supplementary regimen" to increase safety and decrease the adverse effect in the treatment of corneal infections.

Cited by 32 publications

References 56 publications

Resveratrol Attenuates Acute Inflammatory Injury in Experimental Subarachnoid Hemorrhage in Rats via Inhibition of TLR4 Pathway

Resveratrol Attenuates Acute Inflammatory Injury in Experimental Subarachnoid Hemorrhage in Rats via Inhibition of TLR4 Pathway

Development of ciprofloxacin-loaded contact lenses using fluorous chemistry

Resveratrol Restores LINE-1 Methylation Levels by Modulating SIRT1 and DNMTs Functions in Cellular Models of Age-Related Macular Degeneration

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