The effect of repeated cryopreservation and thawing using cryotip on the clinical outcomes of embryos

Mizobe, Yamato; Kuwatsuru, Yukari; Kuroki, Y.; Fukumoto, Yumiko; Tokudome, Mari; Moewaki, Harue; Watanabe, Mia; Tabira, Tatsunori; Iwakawa, Tokiko; Takeuchi, Kazuhiro

doi:10.1002/rmb2.12365

Cited by 11 publications

(7 citation statements)

References 26 publications

(35 reference statements)

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“…However, the developmental potential of embryos subjected to repeated freezing and thawing could be impaired despite the use of vitrification, a safe cryopreservation protocol. At present, most studies have observed that the pregnancy rate of twice-vitrified embryo transfer is comparable to the implantation and pregnancy rates of once-vitrified embryo transfer, which supports the safety and reliability of repeated vitrification [6][7][8][9][10]. In contrast, Aluko et al [11] have observed that in the PGT-A (preimplantation genetic testing for aneuploid cycle), biopsied blastocysts subjected to double vitrification had lower clinical pregnancy and live birth rates than once-vitrification blastocysts.…”

Section: Discussionmentioning

confidence: 86%

Effects of Repeated Cryopreservation of Cleavage-Stage Embryos on Pregnancy and Perinatal Outcomes of Single Embryo Transfer

Wang¹,

Chen²,

Cheng³

et al. 2022

Clin. Exp. Obstet. Gynecol.

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Background: Embryo cryopreservation is an important part of assisted reproductive technologies to increase cumulative pregnancy rate in clients. However, in clinical settings, embryos can be subjected to repeated cryopreservation-warming cycles due to certain clinical circumstances. There are limited data on the effect such cycles may have on the success of embryo transfer procedures. In this study, we investigated the effect of repeated cryopreservation of cleavage-stage embryos on the pregnancy and perinatal outcomes of frozen embryo transfer (FET). Methods: We retrospectively collected data on single cleavage-stage embryo transfer (ET) cycles performed in our center from January 2017 to August 2021 and identified 98 cycles of repeated cryopreservation-warming in cleavage-stage ET cycles. Propensity score matching was conducted according to the age of the patients, body mass index, endometrial thickness, type of oocyte retrial cycle, and quality of the transplanted embryos to match once-cryopreserved cleavage-stage ET cycles in our center at a ratio of 1:3 (caliper value 0.01). Finally, 294 once-cryopreserved cleavage-stage ET cycles were set as controls. Clinical pregnancy, early miscarriage, live birth rates, and other pregnancy and perinatal outcomes were compared between the two groups. Results: Similar success rates were obtained with twice-cryopreserved embryo transfers (n = 98) and with once-cryopreserved embryo transfers (n = 294). Importantly, there was no significant difference in the clinical pregnancy, early miscarriage, and live birth rates as well as perinatal outcomes such as preterm birth and pregnancy complication rates between the two groups. There were no birth defects in the two groups. Conclusions: Repeated cryopreservation may not negatively affect pregnancy and perinatal outcomes of single cleavage-stage ET and may present a safe option for resuscitated cleavage-stage embryos that have been canceled for transfer.

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Section: Discussionmentioning

confidence: 86%

Effects of Repeated Cryopreservation of Cleavage-Stage Embryos on Pregnancy and Perinatal Outcomes of Single Embryo Transfer

Wang¹,

Chen²,

Cheng³

et al. 2022

Clin. Exp. Obstet. Gynecol.

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“…Our clinic has achieved favorable pregnancy rates with re‐cryopreserved and rethawed blastocyst transfer using CryoTip. 25 Therefore, in the re‐cryopreservation and rethawing of blastocysts, biopsy can be performed without any decrease in the quality of blastocysts. The second reason is the TE biopsy technique.…”

Section: Discussionmentioning

confidence: 99%

“…The first is the establishment of cryopreservation and thawing techniques. Our clinic has achieved favorable pregnancy rates with re‐cryopreserved and rethawed blastocyst transfer using CryoTip 25 …”

Section: Discussionmentioning

confidence: 99%

The effects of differences in trophectoderm biopsy techniques and the number of cells collected for biopsy on next‐generation sequencing results

Mizobe¹,

Kuwatsuru²,

Kuroki³

et al. 2022

Reprod Medicine & Biology

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Purpose To examine how differences in trophectoderm biopsy techniques affect the frequency of mosaic embryos and sequencing results. Methods We examined differences in next‐generation sequencing (NGS) analysis results among operators or according to biopsy technique. Additionally, we determined the cut‐off for the number of collected cells to predict the occurrence of mosaicism. We collected cells according to the cut‐off value and examined whether there was a difference in the NGS analysis results between the pulling and flicking methods. Results There was no difference in the NGS analysis results among the operators. Regarding re‐biopsy, changes in the mosaic were observed in all specimens. The cut‐off value for the number of collected cells was five, and when more than five cells were collected, there was no difference in the NGS analysis results between the two methods. Conclusions We demonstrated that if trophectoderm biopsy techniques and NGS are stable, the cell collection location has a greater effect on NGS results than the biopsy technique.

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“…These results demonstrate that biopsies performed via the novel extrusion method did not affect the recovery of frozen–thawed blastocysts. Furthermore, a good pregnancy rate has been reported with refrozen–thawed embryos using a CryoTip, 11 suggesting the usefulness of performing biopsy using the n‐Ext method and freeze–thawing with a CryoTip.…”

Section: Discussionmentioning

confidence: 99%

A novel trophectoderm biopsy technique for all blastocyst stages

Mizobe¹,

Kuwatsuru²,

Kuroki³

et al. 2021

Reprod Medicine & Biology

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Purpose This study was conducted to assess the effectiveness of a new trophectoderm (TE) biopsy method that does not require prior opening of the zona pellucida at the blastocyst stage. Methods TE biopsy was conducted using a modified extrusion method for embryos during the cleavage stage. In this method, culture medium was injected into the perivitelline space to help extrude TE cells from the zona pellucida before TE biopsy. Results Our extrusion method preserves the embryo culture environment until immediately before biopsy because it does not require opening of the zona pellucida prior to TE biopsy. Furthermore, this method does not require a waiting time for blastocyst hatching after laser irradiation, thereby minimizing damage to the embryos and maintaining the time schedule of culture operations. Conclusions TE biopsy using this novel extrusion method may be useful in various applications, including the collection of TE cells for next‐generation sequencing analysis.

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The effect of repeated cryopreservation and thawing using cryotip on the clinical outcomes of embryos

Cited by 11 publications

References 26 publications

Effects of Repeated Cryopreservation of Cleavage-Stage Embryos on Pregnancy and Perinatal Outcomes of Single Embryo Transfer

Effects of Repeated Cryopreservation of Cleavage-Stage Embryos on Pregnancy and Perinatal Outcomes of Single Embryo Transfer

The effects of differences in trophectoderm biopsy techniques and the number of cells collected for biopsy on next‐generation sequencing results

A novel trophectoderm biopsy technique for all blastocyst stages

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