The effect of repeated administration of methotrexate (MTX) on rat ovary: measurement of serum antimullerian hormone (AMH) levels

Benian, Ali; Güralp, Onur; Uzun, Duygu; Okyar, Alper; Şahmay, Sezai

doi:10.3109/09513590.2012.738725

Cited by 17 publications

(16 citation statements)

References 20 publications

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“…As a folic acid antagonist and inhibitor of DNA synthesis, MTX functions by targeting actively proliferating cells, and in the case of an ectopic pregnancy, impedes further growth of the fetal cells. However, the impact of MTX on other dividing cells, such as oocytes and granulosa cells, is unclear [7].…”

Section: Introductionmentioning

confidence: 99%

Methotrexate does not affect ovarian reserve or subsequent assisted reproductive technology outcomes

Boots¹,

Hill²,

Feinberg

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this research was to study whether methotrexate (MTX) as treatment for ectopic pregnancy (EP) impacts the future fertility of women undergoing assisted reproductive technology (ART) Methods In a systematic review and multi-center retrospective cohort from four academic and private fertility centers, 214 women underwent an ART cycle before and after receiving MTX as treatment for an EP. Measures of ovarian reserve and responsiveness and rates of clinical pregnancy (CP) and live birth (LB) were compared in the ART cycles prior and subsequent to MTX. Results Seven studies were identified in the systematic review, and primary data from four institutions was included in the final analysis. Women were significantly older in post-MTX cycles (35.3 vs 34.7 years). There were no differences in follicle stimulating hormone, antral follicle count, duration of stimulation, oocytes retrieved, or fertilization rate between pre-and post-MTX cycles. However, post-MTX cycles received a significantly higher total dose of gonadotropins (4206 vs 3961 IU). Overall, 42 % of women achieved a CP and 35 % achieved a LB in the post-MTX ART cycle, which is similar to national statistics. Although no factors were identified that were predictive of LB in young women, the number of oocytes retrieved in the previous ART cycle and current AFC were predictive of LB (AUC 0.76, 0.75) for the older women. Conclusions MTX does not influence ovarian reserve, response to gonadotropin stimulation, and CP or LB rate after ART. MTX remains a safe and effective treatment option for women with asymptomatic EPs.Capsule As treatment for an ectopic pregnancy, methotrexate does not affect ovarian reserve, response to gonadotropin stimulation, clinical pregnancy, or live birth rates in subsequent assisted reproductive tenchology cycles.

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Section: Introductionmentioning

confidence: 99%

Methotrexate does not affect ovarian reserve or subsequent assisted reproductive technology outcomes

Boots¹,

Hill²,

Feinberg

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Only one epidemiological study showed that the pregnancy rates of multiple dose MTX were not different from other treatments of EP. Although various studies have attempted to evaluate the effect of single dose MTX on ovarian reserve, there is only one experimental study to evaluate the effect of multiple dose MTX on ovarian reserve with only measuring AMH levels but without control group [12]. Benian et al displayed that there was no significant difference in final AMH levels after multiple dose MTX protocol.…”

Section: Resultsmentioning

confidence: 99%

“…AMH was used as a useful biomarker to show ovarian reserve after MTX administration for EP [2]. A recent study in a rat model suggested that MTX administration in repeated doses did not decrease AMH levels [12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the effects of single or multiple dose methotrexate administration, salpingectomy on ovarian reserve of rat with the measurement of anti-Müllerian hormone (AMH) levels and histological analysis

Ulug

Öner

2014

European Journal of Obstetrics & Gynecology and Reproductiv

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“…Methods used to determine the ovarian reserve include a primordial follicle count, the amount of gonadotropins and the level of anti-Müllerian hormone. 5 However, the occurrence of pregnancy is the best clinical parameter illustrating the ovarian reserve and the fertility of a female.…”

Section: Introductionmentioning

confidence: 99%

An experimental study on the use of lycopene to prevent infertility due to acute oxidative ovarian damage caused by a single high dose of methotrexate

Turkler¹,

Onat²,

Yıldırım³

et al. 2020

Adv Clin Exp Med

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Background. Methotrexate (MTX) is an antineoplastic agent, which increases the level of reactive oxygen species (ROS) and decreases the level of antioxidants. Lycopene, is a potent antioxidant, which is used because of its protective effect against tissue damage.Objectives. The aim of this study was to determine the effect of lycopene on ovarian MTX-induced injury in rats.Material and methods. Rats (n = 36) were randomly divided into 3 equal groups: a group with MTX only (MG, n = 12), a group with lycopene and MTX (LMG, n = 12), and a healthy control group (HCG, n = 12). Then, malondialdehyde (MDA), myeloperoxidase (MPO) and total glutathione (tGSH) levels and histopathological findings were examined in the ovaries of rats. Apart from the histopathological and biochemical evaluation, the reproductive performance of the experimental groups was also examined. Results.Our study demonstrated that, in ovarian tissues of rats administered MTX, there was a decrease in the levels of tGSH, while MDA and MPO were increased, but it is observed that these ratios are reversed in the LMG (p < 0.05). It also has been proven that a single, high-dose use of MTX causes infertility in female rats, prolongs the gestation period and reduces the number of offspring. Conclusions.Lycopene pretreatment ameliorates the MTX induced ovarian injury and infertility in rats through its antioxidative activities.

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The effect of repeated administration of methotrexate (MTX) on rat ovary: measurement of serum antimullerian hormone (AMH) levels

Abstract: MTX administration did not cause a statistically significant change between pretreatment and final serum AMH levels in rats. There was no decrease in AMH levels indicating a decrease in ovarian reserve.

Cited by 17 publications

References 20 publications

Methotrexate does not affect ovarian reserve or subsequent assisted reproductive technology outcomes

Methotrexate does not affect ovarian reserve or subsequent assisted reproductive technology outcomes

Evaluation of the effects of single or multiple dose methotrexate administration, salpingectomy on ovarian reserve of rat with the measurement of anti-Müllerian hormone (AMH) levels and histological analysis

An experimental study on the use of lycopene to prevent infertility due to acute oxidative ovarian damage caused by a single high dose of methotrexate

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