The Effect of Relative Needle Diameter in Puncture and Sham Injection Animal Models of Degeneration

Elliott, Dawn M.; Yerramalli, Chandra Sekher; Beckstein, Jesse C.; Boxberger, John I.; Johannessen, Wade; Vresilovic, Edward J.

doi:10.1097/brs.0b013e318166e0a2

Cited by 209 publications

(234 citation statements)

References 53 publications

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“…[36][37][38] Establishing a relationship between disc behavior and external osmotic pressure will be helpful for measuring intradiscal pressure without the use of pressure sensors, which might alter disc mechanics because of annular injuries. 39 The bovine caudal disc is an ideal animal model to study disc mechanics of the healthy human disc, based on similarities in biomechanical composition, disc height, swelling pressure, and mechanical properties. [30][31][32][33]40 However, the bovine discs do not exhibit signs of degeneration or aging like the human disc.…”

Section: Discussionmentioning

confidence: 99%

Osmotic Pressure Alters Time-dependent Recovery Behavior of the Intervertebral Disc

Bezci

O’Connell

2018

Spine

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Study Design. Disc recovery behavior under hypo-and hyperosmotic pressure. Objective. To evaluate the effect of osmotic pressure on the unloaded recovery response of healthy discs. Summary of Background Data. The intervertebral disc is a poroviscoelastic material that experiences large fluctuations in water composition throughout a diurnal loading cycle. Fluid flow out of the disc occurs through mechanical loading, whereas fluid flow into the disc occurs through passive diffusion because of an imbalance of ions between the disc and its surrounding environment. Osmotic pressure has been used to alter water uptake and tissue hydration. Methods. Motion segments were prepared from the caudal spine sections of the skeletally mature bovines. A 300-N compressive load was applied for 2 hours before unloaded recovery for 12 hours. Hypo-and hyperosmotic pressure was used to alter the rate of water uptake and disc height recovery during unloaded recovery. A 5-parameter rheological model was used to describe the disc's time-dependent recovery behavior. Results. The elastic response was not altered by changes in osmotic pressure; however, viscoelastic recovery was highly dependent on saline osmolarity and recovery time. The fast response of viscoelastic recovery was not dependent on osmotic pressure. The time constant for the slow response decreased whereas the slow response stiffness increased as osmotic pressure increased.Conclusion. The fast response of viscoelastic recovery is governed by flow-independent recovery, whereas the slow response is related to flow-dependent recovery. The rate and magnitude of flow-dependent recovery are highly sensitive to changes in osmotic pressure of the saline bath. There is an osmotic pressure that reduces disc recovery behavior to an elastic response or flow-independent recovery.

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Section: Discussionmentioning

confidence: 99%

Osmotic Pressure Alters Time-dependent Recovery Behavior of the Intervertebral Disc

Bezci

O’Connell

2018

Spine

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“…This information would give an indication of whether the cells were alive and functioning and whether they were producing appropriate matrix macromolecules to restore disc height and biomechanical behaviour. Cartilage repair has been assessed through biopsies but since even needle puncture, depending on its size, can lead to disc degeneration [25,54] biopsies are unlikely to be used to assess disc repair clinically.…”

Section: How Do We Assess Success?mentioning

confidence: 99%

Tissue engineering and the intervertebral disc: the challenges

2008

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Disc degeneration is a common disorder. Although the back pain that can develop in association with this is rarely life-threatening, the annual cost in terms of morbidity, lost productivity, medical expenses and workers' compensation benefits is significant. Surgical intervention as practised currently is directed towards removing the damaged or altered tissue. Development of new treatment modalities is critical as there is a growing consensus that the strategies used currently for symptomatic degenerative disc disease may not be effective. Accordingly, there is a need to develop an entirely new way to treat this disorder; regenerative medicine and tissue engineering approaches appear particularly promising in this regard. This paper reviews some of the challenges that currently are limiting the clinical application of this approach to the treatment of disc degeneration.

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“…Elliot et al 11 suggested that needle puncture inducing discal changes is dependent on the ratio of needle diameter to the disc height. In this case, disc changes were universal for the ratio over 40%.…”

Section: Discussionmentioning

confidence: 99%

Does a small size needle puncture cause intervertebral disc changes?

Issy

Castania²,

Silveira³

et al. 2015

Acta Cir. Bras.

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PURPOSE: Small size needles have been regularly used for intradiscal injection of innocuous/potential therapeutic compounds in experimental conditions, but also in clinic procedures, such as discography. Our aim was to investigate if a 30-gauge needle could trigger observable changes on intact intervertebral discs. We compared these effects to those induced by a large size needle (21-gauge), a well-known intervertebral disc degenerative model based on needle puncture. METHODS:Coccygeal intervertebral discs (Co8-9) of adult male Wistar rats were punctured with a 21-gauge needle, while the coccygeal levels Co7-8 and Co9-10 remained intact. The 30-gauge needle was used to inject a safe volume of saline (2 µl) on both intact (Co9-10) and punctured (Co8-9) discs. MRI and histological score were performed at 2, 15 and 42 days after procedure. RESULTS:MRI analyses revealed significant reduction on signal intensity of 21-gauge punctured discs. Intact discs which received a saline injection through a 30-gauge needle also revealed significant alterations in the MRI signal when compared with control discs. No histological changes were observed in the intact saline injected discs at any time analyzed.CONCLUSION: Since significant intervertebral image changes were observed with a 30-gauge needle, cautious interpretation of the pharmacological inoculation findings is required.

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The Effect of Relative Needle Diameter in Puncture and Sham Injection Animal Models of Degeneration

Cited by 209 publications

References 53 publications

Osmotic Pressure Alters Time-dependent Recovery Behavior of the Intervertebral Disc

Osmotic Pressure Alters Time-dependent Recovery Behavior of the Intervertebral Disc

Tissue engineering and the intervertebral disc: the challenges

Does a small size needle puncture cause intervertebral disc changes?

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