The effect of redox environment on l -lactic acid production by Lactobacillus paracasei —A proof by genetically encoded in vivo NADH biosensor

Tian, Xiaohui; Zhang, Ning; Yang, Yi; Wang, Yonghong; Chu, Ju; Zhuang, Yingping; Zhang, Siliang

doi:10.1016/j.procbio.2015.10.001

Cited by 11 publications

(5 citation statements)

References 31 publications

(35 reference statements)

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“…Consortium‐based CBP of lignocellulosic biomass to lactic acid requires at least one microorganism for the production of cellulolytic enzymes and one strain that converts the sugars released to lactic acid. Many lactic acid bacteria (LAB) are known for microaerophilic or anaerobic fermentations (Tian et al, ). In contrast aerobic fungi such as T. reesei are primarily used for the industrial production of cellulolytic enzymes (Geddes et al, ; Sharma, Tewari, Rana, Soni, & Soni, ).…”

Section: Resultsmentioning

confidence: 99%

Consolidated bioprocessing of lignocellulosic biomass to lactic acid by a synthetic fungal‐bacterial consortium

Shahab

Luterbacher

Brethauer

et al. 2018

Biotech & Bioengineering

106

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Consolidated bioprocessing (CBP) of lignocellulosic feedstocks to platform chemicals requires complex metabolic processes, which are commonly executed by single genetically engineered microorganisms. Alternatively, synthetic consortia can be employed to compartmentalize the required metabolic functions among different specialized microorganisms as demonstrated in this work for the direct production of lactic acid from lignocellulosic biomass. We composed an artificial cross-kingdom consortium and co-cultivated the aerobic fungus Trichoderma reesei for the secretion of cellulolytic enzymes with facultative anaerobic lactic acid bacteria. We engineered ecological niches to enable the formation of a spatially structured biofilm. Up to 34.7 gL lactic acid could be produced from 5% (w/w) microcrystalline cellulose. Challenges in converting pretreated lignocellulosic biomass include the presence of inhibitors, the formation of acetic acid and carbon catabolite repression. In the CBP consortium hexoses and pentoses were simultaneously consumed and metabolic cross-feeding enabled the in situ degradation of acetic acid. As a result, superior product purities were achieved and 19.8 gL (85.2% of the theoretical maximum) of lactic acid could be produced from non-detoxified steam-pretreated beech wood. These results demonstrate the potential of consortium-based CBP technologies for the production of high value chemicals from pretreated lignocellulosic biomass in a single step.

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Section: Resultsmentioning

confidence: 99%

Consolidated bioprocessing of lignocellulosic biomass to lactic acid by a synthetic fungal‐bacterial consortium

Shahab

Luterbacher

Brethauer

et al. 2018

Biotech & Bioengineering

106

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“…NAD is a cofactor in essential biological processes, and Lactobacillus casei 12A contains two potential NADH dehydrogenases: the one targeted here and another located in cluster with a putative pheromone precursor. The ratio of NAD ϩ to NADH plays an important role in regulating the intracellular redox state and the metabolism of cells (48,49). Metabolism of carbon sources (e.g., glucose) during growth results in the reduction of NAD ϩ to NADH.…”

Section: Discussionmentioning

confidence: 99%

Transient MutS-Based Hypermutation System for Adaptive Evolution of Lactobacillus casei to Low pH

Overbeck

Welker

Hughes

et al. 2017

Appl Environ Microbiol

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This study explored transient inactivation of the gene encoding the DNA mismatch repair enzyme MutS as a tool for adaptive evolution of MutS deletion derivatives of 12A and ATCC 334 were constructed and subjected to a 100-day adaptive evolution process to increase lactic acid resistance at low pH. Wild-type parental strains were also subjected to this treatment. At the end of the process, the Δ lesion was repaired in representative 12A and ATCC 334 Δ mutant isolates. Growth studies in broth at pH 4.0 (titrated with lactic acid) showed that all four adapted strains grew more rapidly, to higher cell densities, and produced significantly more lactic acid than untreated wild-type cells. However, the adapted Δ derivative mutants showed the greatest increases in growth and lactic acid production. Further characterization of the 12A-adapted Δ derivative revealed that it had a significantly smaller cell volume, a rougher cell surface, and significantly better survival at pH 2.5 than parental 12A. Genome sequence analysis confirmed that transient inactivation decreased DNA replication fidelity in both strains, and it identified genetic changes that might contribute to the lactic acid-resistant phenotypes of adapted cells. Targeted inactivation of three genes that had acquired nonsense mutations in the adapted 12A Δ mutant derivative showed that NADH dehydrogenase (), phosphate transport ATP-binding protein PstB (), and two-component signal transduction system (TCS) quorum-sensing histidine protein kinase () genes act in combination to increase lactic acid resistance in 12A. Adaptive evolution has been applied to microorganisms to increase industrially desirable phenotypes, including acid resistance. We developed a method to increase the adaptability of 12A and ATCC 334 through transient inactivation of the DNA mismatch repair enzyme MutS. Here, we show this method was effective in increasing the resistance of to lactic acid at low pH. Additionally, we identified three genes that contribute to increased acid resistance in 12A. These results provide valuable insight on methods to enhance an organism's fitness to complex phenotypes through adaptive evolution and targeted gene inactivation.

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“…Caulobacter crescentus E GSH roGFP2 [233] Chlamydia trachomatis E GSH roGFP2 [234] Citrobacter rodentium E GSH roGFP2 [235] Corynebacterium glutamicum E MSH Mrx1-roGFP2 [74] Escherichia coli E GSH rxYFP [93] roGFP1 [236] roGFP2 [235,[237][238][239] Grx1-roGFP2 [239,240] general redox state roUnaG [99] H 2 O 2 roGFP2-Orp1 [239,240] Sand R-MetO MetSOx, MetROx [113] Lactobacillus paracasei NADH Frex [241] Lactococcus lactis E GSH roGFP1-R12 [242] Methylococcus capsulatus…”

Section: Bacteriamentioning

confidence: 99%

“…Compared to Peredox, Frex exhibits lower affinity which prevents its saturation under typical bacterial NADH levels. Frex has also been used to monitor L-lactic acid production by Lactobacillus paracasei [241]. Using Frex, Tian et al found values of extracellular oxidative and reductive potential, under which L-lactic acid production during the cell growth and stationary phases could increase significantly.…”

Section: Biotechnologymentioning

confidence: 99%

In Vivo Imaging with Genetically Encoded Redox Biosensors

Kostyuk

Panova

Kokova

et al. 2020

IJMS

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Redox reactions are of high fundamental and practical interest since they are involved in both normal physiology and the pathogenesis of various diseases. However, this area of research has always been a relatively problematic field in the context of analytical approaches, mostly because of the unstable nature of the compounds that are measured. Genetically encoded sensors allow for the registration of highly reactive molecules in real-time mode and, therefore, they began a new era in redox biology. Their strongest points manifest most brightly in in vivo experiments and pave the way for the non-invasive investigation of biochemical pathways that proceed in organisms from different systematic groups. In the first part of the review, we briefly describe the redox sensors that were used in vivo as well as summarize the model systems to which they were applied. Next, we thoroughly discuss the biological results obtained in these studies in regard to animals, plants, as well as unicellular eukaryotes and prokaryotes. We hope that this work reflects the amazing power of this technology and can serve as a useful guide for biologists and chemists who work in the field of redox processes.

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The effect of redox environment on l -lactic acid production by Lactobacillus paracasei —A proof by genetically encoded in vivo NADH biosensor

Cited by 11 publications

References 31 publications

Consolidated bioprocessing of lignocellulosic biomass to lactic acid by a synthetic fungal‐bacterial consortium

Consolidated bioprocessing of lignocellulosic biomass to lactic acid by a synthetic fungal‐bacterial consortium

Transient MutS-Based Hypermutation System for Adaptive Evolution of Lactobacillus casei to Low pH

In Vivo Imaging with Genetically Encoded Redox Biosensors

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