The effect of Ramadan fasting on sirtuin and visfatin levels

Abstract

Abstract:Objective: Our aim was to determine how Ramadan fasting eff ects serum Human NAD-dependent deacetylase sirtuin 1 (SIRT1/ SIR2L1) and visfatin levels during pregnancy. Materials and methods: Thirty-six patients were included in this case-control study. The patient group consisted of 18 pregnant women who were Ramadan fasting. Another healthy 18 pregnant women with matching pregnancy weeks and ages formed the control group. Blood samples were obtained from the patients for biochemical analyses and serum adipokine level measurements. Results: The mean sirtuin and visfatin levels in fasting pregnant women were 2.87 ± 0.95 and 60.18 ± 19.49 ng/mL, respectively, while the mean serum sirtuin and visfatin levels in the control group was determined to be 4.28 ± 1.45 and 23.26 ± 6.18 ng/mL, respectively. A statistically signifi cant diff erence was determined between the groups (p = 0.002, p = 0.0001). A correlation analysis provided a negative correlation between number of fasting days and sirtuin levels (r = 0.45, p = 0.005) and a positive correlation with visfatin levels (r = 0.73, p = 0.0001). Conclusion: As a result, while the serum sirtuin 1 level of pregnant women decreased due to fasting in Ramadan, we determined that their visfatin levels increased and this increase was in correlation with the days of fasting.

Chrononutrition during Pregnancy and Its Association with Maternal and Offspring Outcomes: A Systematic Review and Meta-Analysis of Ramadan and Non-Ramadan Studies

“…Five studies explored the association of Ramadan fasting during pregnancy with fasting blood glucose [ 19 , 53 , 54 , 55 , 56 ]. Results of our meta-analysis showed that fasting during Ramadan significantly reduced fasting blood glucose by 2.73 mg/dl (95% CI: −5.23, −0.22) compared with the non-fasting group ( I 2 = 34.44%) ( Figure 6 ).…”

Expression Profile of Diabetes-Related Genes Associated with Leukocyte Sirtuin 1 Overexpression in Gestational Diabetes

“…Although calorie restriction (CR) has been proven to extend the lifespan in lower organisms and mammals through its effect on sirtuins, as evidenced by increased SIRT1 expression in the adipose tissue, liver, kidney, and brain tissue of rodents, as well as in PBMC [33,34,35], it has also been found that the plasma SIRT1 concentration falls in pregnant women during Ramadan fasting to a degree that correlates inversely with the number of fasting days [36]. Physical activity has also been documented as a positive regulator of SIRT1 expression in rats and humans [37,38].…”

Detection of Sirtuin-1 protein expression in peripheral blood leukocytes in dogs

“…In this study, we examined intracellular SIRT1 level in peripheral blood lymphocytes for ease of access in clinical cases. Though some studies measured the serum SIRT1 concentration to evaluate the SIRT1 expression level [ 10 , 24 , 29 , 32 , 52 ], the significance of the plasma SIRT1 concentration remains to be clarified because SIRT1 localizes mainly in the nucleus or the cytoplasm. SIRT1 in the cellular lysate and in the serum can potentially be measured by enzyme linked immunosorbent assay (ELISA) in dogs.…”

“…Though its enzymatic activity has been measured to evaluate its potential inhibitors and activators using cultured cells or recombinant SIRT1 [ 14 ] with respect to longevity, it is usually difficult to evaluate its activity in tissue samples obtained from patients. Thus SIRT1 expression was evaluated by measuring mRNA transcription, tissue SIRT1 protein expression or plasma SIRT1 concentration in association with nutritional conditions or diseases [ 10 , 21 , 23 , 24 , 29 , 35 , 36 , 38 , 49 ]. Not only SIRT1 activation but also increasing SIRT1 expression is related to lifespan extension in experimental condition [ 2 ].…”