The Effect of Radiations on Galactozymase Formation in Yeast

Brandt, C. L.; Freeman, Paul J; Swenson, P. A.

doi:10.1126/science.113.2936.383

Cited by 24 publications

(8 citation statements)

References 7 publications

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“…Effect of ultraviolet irradiation on the process of citrate adaptation. Ultraviolet light is known to block the formation of adaptive enzymes at levels of irradiation which do not harm preformed enzymes (Swenson and Giese, 1950;Brandt et al, 1951;Entner and Stanier, 1951). Irradiation was carried out empirically as described by Entner and Stanier (1951), and the effect of such irradiation is illustrated in figure 3.…”

Section: Resultsmentioning

confidence: 99%

The Nature of the Adaptive Lag of Pseudomonas Fluorescens Toward Citrate

1953

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Section: Resultsmentioning

confidence: 99%

The Nature of the Adaptive Lag of Pseudomonas Fluorescens Toward Citrate

1953

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“…Jacob, Torriani & Monod (1951) found that doses which inhibit adaptation in bacteria do not affect the ability of the cells to reproduce bacteriophage. Brandt, Freeman & Swenson (1951) found that ultraviolet irradiation inhibited the formation of galactozymase in yeast, but had no effect on the activity of preformed galactozymase, or on glucozymase. Swenson (1952) determined the action spectrum of inhibition of galactozymase formation by ultraviolet irradiation, and found that it had a maximum at 260 m,.…”

Section: Oxidation Of Tricarboxylic Acid-cycle Intermediates By Washementioning

confidence: 96%

Oxidative pathways in a fluorescent Pseudomonas

Kogut

Podoski

1953

Biochemical Journal

110

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“…Since methylene blue molecules can be reduced, and reoxidized, while absorbed on the cell surface, this would indicate that the cell membrane could be the predominant site of the injury. Brandt et al (22) studied the effect of radiations on galactozymase formation in yeast. Using rose bengal as a photosensitizer, they observed that, after the exposure to light, entry of materials into the cell was in some way reduced or prevented.…”

Section: Bo + DXmentioning

confidence: 99%

Studies on the Mechanism of the Photosensitized Inactivation of E. Coli and Reactivation Phenomenon

Heinmets¹,

Vinegar²,

Taylor³

1952

Journal of General Physiology

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Photosensitized inactivation of microorganisms has been the subject of numerous studies, and various hypothetical reaction mechanisms have been proposed for the interpretation of the phenomenon. In spite of such extensive experimentation and theorizing, there still exists uncertainty concerning the inactivation processes. No attempt will be made to review the subject since it has been analyzed and treated by Blum (1) and Arnow (2). It is evident that photosensitized inactivation is dependent on a variety of experimental factors. However, the literature reveals that the influence of such factors has been determined in many diverse and isolated experiments which makes the over-all evaluation of the data difficult, It was thought that, by studying a single system under various experimental conditions, a more satisfactory interpretation could be found. A methylene blue and E. coli system was selected, since the methylene blue oxidation-reduction cycle represents a reversible process, and the associated structural changes in the molecule are known. Experimental Metkods and Results Experimental Material and Irradiation Procedures.--E. coli B/r were grown inliquid synthetic M-9 medium (3) with aeration. The culture was centrifuged and resuspended in the desired solvent. Mter irradiation, assays were made by diluting the suspension in saline and plating on nutrient agar containing 4 × 10 -6 gin. per ml. of gentian violet.G. E. H-4 and DH-1 mercury lamps were used as irradiation sources. The test tubes containing the samples were placed in a water bath 5 cm. from the source. During the irradiation oxygen bubbled through the solution, providing a constant oxygen tension and continuous agitation of the suspension. Occasionally, different procedures were used. These will be described in other sections of the paper.The methylene blue used was u.s.1,, grade commercial product which contained not less than 98.5 per cent methylene blue chloride.

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The Effect of Radiations on Galactozymase Formation in Yeast

Cited by 24 publications

References 7 publications

The Nature of the Adaptive Lag of Pseudomonas Fluorescens Toward Citrate

The Nature of the Adaptive Lag of Pseudomonas Fluorescens Toward Citrate

Oxidative pathways in a fluorescent Pseudomonas

Studies on the Mechanism of the Photosensitized Inactivation of E. Coli and Reactivation Phenomenon

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