The Effect of Quercetin on the Osteogenesic Differentiation and Angiogenic Factor Expression of Bone Marrow-Derived Mesenchymal Stem Cells

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are widely used in regenerative medicine in light of their ability to differentiate along the chondrogenic and osteogenic lineages. As a type of traditional Chinese medicine, quercetin has been preliminarily reported to promote osteogenic differentiation in osteoblasts. In the present study, the effects of quercetin on the proliferation, viability, cellular morphology, osteogenic differentiation and angiogenic factor secretion of rat BMSCs (rBMSCs) were examined by MTT assay, fluorescence activated cell sorter (FACS) analysis, real-time quantitative PCR (RT-PCR) analysis, alkaline phosphatase (ALP) activity and calcium deposition assays, and Enzyme-linked immunosorbent assay (ELISA). Moreover, whether mitogen-activated protein kinase (MAPK) signaling pathways were involved in these processes was also explored. The results showed that quercetin significantly enhanced the cell proliferation, osteogenic differentiation and angiogenic factor secretion of rBMSCs in a dose-dependent manner, with a concentration of 2 μM achieving the greatest stimulatory effect. Moreover, the activation of the extracellular signal-regulated protein kinases (ERK) and p38 pathways was observed in quercetin-treated rBMSCs. Furthermore, these induction effects could be repressed by either the ERK inhibitor PD98059 or the p38 inhibitor SB202190, respectively. These data indicated that quercetin could promote the proliferation, osteogenic differentiation and angiogenic factor secretion of rBMSCs in vitro, partially through the ERK and p38 signaling pathways.

Proliferation and osteogenic differentiation of rat BMSCs on a novel Ti/SiC metal matrix nanocomposite modified by friction stir processing

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“…The expression levels of the angiogenesis-related markers VEGF, ANG-1 and HIF-1α were detected. It is well known that these angiogenic factors could simultaneously promote osteogenesis and angiogenesis8386. VEGF is a key angiogenic factor for enhancing blood vessel formation that effectively regulates biological activity87.…”

Effect of micro-nano-hybrid structured hydroxyapatite bioceramics on osteogenic and cementogenic differentiation of human periodontal ligament stem cell via Wnt signaling pathway

“…As previously reported, the cells were seeded in 96-well plates with different densities of 5×10 3 , 1×10 4 , 1.5×10 4 , 2×10 4 , and 2.5×10 4 cell/well and cultured in α-MEM, and then the cell number was quantified by a cell standard curve. 32 All experiments were conducted in triplicate.…”

Effects of Nicotinamide on Cytotoxicity-induced Morphological Changes in Osteoblastic Cells <i>In Vitro</i>

“…This method is often used in studies on the proliferation and differentiation of osteoblasts [10][11][12][13][14] . In addition, they are used in studies involving bone metabolism and biomaterials [15][16][17][18][19] .…”