The effect of quercetin in the maturation media on cumulus-granulosa cells and the developmental competence of bovine oocytes

Davoodian, Najmeh; Kadivar, Ali; Davoodian, Nahid; Ahmadi, Ebrahim; Nazari, Hassan; Mehrban, Hossein

doi:10.1016/j.theriogenology.2022.06.026

Cited by 5 publications

(7 citation statements)

References 69 publications

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“…ORY reduced the level of ROS, which was concomitant with the downregulation of proapoptotic genes. Furthermore, ORY‐treated oocytes showed higher quality, as detected by higher levels of IGF2R and OCT‐4 expression, as the selected candidates to evaluate the potential of matured oocytes to grow and differentiate (Agarwal et al., 2006; Davoodian et al., 2022; Esfandiari et al., 2005; Sutton et al., 2003).…”

Section: Discussionmentioning

confidence: 99%

“…The nuclear status was assessed as described in previous studies (Davoodian et al., 2022; Fatehi et al., 2005; Mori et al., 1988). Mature bovine oocytes were denuded of cumulus cells, washed in HEPES‐buffered medium 199, transferred to drops of 2 mM Hoechst 33,342 for 5 min, then mounted on a glass slide, covered by a coverslip, and observed under an epifluorescent microscope.…”

Section: Methodsmentioning

confidence: 99%

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Supplementation of media with gamma‐oryzanol as a novel antioxidant to overcome redox imbalance during bovine oocyte maturation in vitro

Davoodian,

Kadivar,

Mehrban

2023

Reprod Domestic Animals

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This study evaluated the effect of supplementing IVM media with γ‐oryzanol (ORY), a nutraceutical derived from rice bran oil, on the development of bovine oocytes and hindering the compromising effect of redox imbalance. An in vitro model of the bovine cumulus‐oocyte complex was used for the evaluation of nuclear maturation and development. Antioxidant activity was investigated by assessing the level of ROS (Reactive Oxygen Species) and GSH (glutathione) in oocytes and quantitative changes in gene expression in matured oocytes and their respective cumulus cells. ORY supplementation increased the proportion of MII oocytes, cleaved embryos, and total blastocysts (p < .05) and was linked to higher and lower levels of intracellular GSH and ROS, respectively (p < .05). The treated oocytes and their respective cumulus‐granulosa cells showed a modulation in the expression of genes related to apoptosis (downregulation of BAX and CHOP) and oxidative stress (upregulation of NRF2, CAT, and SOD). Also, relative upregulation of OCT‐4 and IGF2R in treated oocytes was concomitant with higher subsequent development in terms of cleavage and total blastocyst rates (p < .05). Based on our findings, it appears that ORY supplementation can improve the nuclear maturation and development of bovine oocytes into blastocysts and augment their enzymatic and non‐enzymatic antioxidant systems, maintaining the Redox balance and high enzymatic activity against ROS generation.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Supplementation of media with gamma‐oryzanol as a novel antioxidant to overcome redox imbalance during bovine oocyte maturation in vitro

Davoodian,

Kadivar,

Mehrban

2023

Reprod Domestic Animals

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“…These results strongly suggested that MAPK/ERK signaling pathway was modulated by quercetin in chicken GCs of the prehierarchical follicles. The previous study showed that supplementing maturation media with quercetin can enhance the quality of bovine oocytes and boost the subsequent development of oocytes to blastocyst against early apoptotic damage mechanisms in granulosa cells ( Davoodian et al, 2022 ). Quercetin can improve ovulation in polycystic ovary syndrome ( Chen et al, 2022a ) and prevent primordial follicle loss in cyclophosphamide-treated mice ( Li et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of quercetin on granulosa cells from prehierarchical follicles by modulating MAPK signaling pathway in chicken

Shen¹,

Li²,

Yuan³

et al. 2023

Poultry Science

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“…11 in vitro studies (Jia et al, 2011;Capcarova et al, 2015;Tarko et al, 2018;Sirotkin et al, 2019a;Sirotkin et al, 2019b;Khadrawy et al, 2019;Kolesarova et al, 2019;Rashidi et al, 2019;Li et al, 2020;Yang et al, 2021b;Davoodian et al, 2022) have been included and are shown in Table 1. The cell origin, in vitro model, QUR dosage and biological response have been reviewed.…”

Section: In Vitro Studies Of Qur On Ovarian Cellsmentioning

confidence: 99%

“…QUR not only relieves cell damage by reducing oxidative stress, but also repairs cells by inhibiting apoptosis. QUR has been found to increase B-cell lymphoma-2 (Bcl-2) and decrease Blc-2 associated X protein (Bax) in ovary cells of cattle, gilts, buffalo, goat, chicken and bovine (Jia et al, 2011;Sirotkin et al, 2019b;Li et al, 2020;Yang et al, 2021b;Davoodian et al, 2022). However, it is difficult to prove that the same effect applies to humans because there is rare research on primary granulosa cells from humans.…”

Section: In Vitro Studies Of Qur On Ovarian Cellsmentioning

confidence: 99%

Quercetin and polycystic ovary syndrome

Ma¹,

Xiang

Song³

et al. 2022

Front. Pharmacol.

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Polycystic ovary syndrome (PCOS) is a reproductive endocrine disease, and results to opsomenorrhea or amenorrhea, hairy, acne, acanthosis, infertility, abortion. In the long term, PCOS may also increase the risk of endometrial cancer, diabetes, hypertension, dyslipidemia and other diseases. Till now there is no specific drug for PCOS due to the unclearness of the cause and pathogenesis, as current treatments for PCOS only target certain symptoms. Quercetin (QUR) is a flavonoid drug widely found in Chinese herbal medicines, fruits, leaves, vegetables, seeds and plants roots. Studies on other diseases have found that QUR has anti-oxidant, anti-inflammatory, anti-insulin resistance, anti-cancer and other effects. Some studies have shown that serum testosterone (T), luteinizing hormone (LH), the LH/follicule-stimulating hormone (FSH) ratio, fasting glucose, fasting insulin, HOMA-IR and lipid levels are reduced in PCOS patients with QUR treatment. However, the mechanisms of QUR in PCOS patients have not been completely elucidated. In this review, we retrospect the basic characteristics of QUR, and in vitro studies, animal experiments and clinical trials of QUR and plant extracts containing QUR in the treatment of PCOS. We also summarized the effects and mechanism of QUR in ovarian cells in vitro and PCOS model rats, the changes in relevant parameters after QUR administration in PCOS patients, and its potentially therapeutic applications.

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The effect of quercetin in the maturation media on cumulus-granulosa cells and the developmental competence of bovine oocytes

Cited by 5 publications

References 69 publications

Supplementation of media with gamma‐oryzanol as a novel antioxidant to overcome redox imbalance during bovine oocyte maturation in vitro

Supplementation of media with gamma‐oryzanol as a novel antioxidant to overcome redox imbalance during bovine oocyte maturation in vitro

Effects of quercetin on granulosa cells from prehierarchical follicles by modulating MAPK signaling pathway in chicken

Quercetin and polycystic ovary syndrome

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