A number of studies stated that nanotechnology is predicted to encourage the third wave of research diffusion, development, and advancement in agricultural and food sectors, as an integrated part of the nanotechnology revolution in various fields. The aim of this study was to assess the effect of nanotechnology knowledge, trust, and communication media on perceived risk and willingness to buy food nanotechnology. The data came from an online questionnaire with 302 people collected by an online survey, and the research used a purposive sampling method, and structural equation modeling was used for data analysis. Based on the result of the research on three variables (nanotechnology knowledge, trust, and communication media), it is known that communication media and trust had significant effects on risk perception; however, nanotechnology knowledge had no significant effect. Furthermore, perceived risk had a significant effect on willingness to buy food nanotechnology. The sequence from the largest to the smallest of willingness to buy is functional food with a health benefit, food packaging, and food additive to improved product quality. Functional food has been chosen because there is an additional health benefit they will get. Meanwhile, food packaging was considered less risky as it did not directly contact with the product and was not digested by the body. Food additives to improve product quality was the lowest since the respondents felt they did not get any benefits from the application.Abstrak: Sejumlah studi menyebutkan bahwa nanoteknologi diprediksi akan mendorong gelombang ketiga difusi penelitian, pengembangan dan kemajuan di sektor pertanian dan pangan, sebagai bagian yang tak terpisahkan dari revolusi nanoteknologi di berbagai bidang. Tujuan penelitian ini adalah menganalisis pengaruh nanotechnology knowledge, trust, dan media komunikasi terhadap persepsi risiko dan willingness to buy pangan nanoteknologi, Pengambilan data menggunakan media kuesioner online dengan responden sebanyak 302 orang yang dikumpulkan dengan metode purposive sampling dan metode structural equation modeling (SEM) untuk menganalisa data. Berdasarkan hasil penelitian dengan tiga variabel yaitu nanotechnology knowledge, trust, dan media komunikasi, diketahui bahwa variabel media komunikasi dan trust memberikan hasil pengaruh secara signifikan terhadap persepsi risiko. Sementara itu, nanotechnology knowledge tidak berpengaruh secara signifikan terhadap persepsi risiko. Lebih lanjut, hasil menunjukkan persepsi risiko berpengaruh secara signifikan terhadap willingness to buy pangan nanoteknologi. Jika dikelompokkan berdasarkan jenis maka urutan willingness to buy terbesar hingga terkecil adalah pangan fungsional dengan manfaat kesehatan, kemasan pangan, dan bahan tambahan pangan untuk memperbaiki kualitas produk. Pangan fungsional dipilih responden dengan alasan adanya tambahan manfaat akan kesehatan. Kemasan pangan dengan aplikasi nanoteknologi dinilai lebih tidak berisiko dikarenakan tidak kontak secara langsung dengan produk dan t...