The Effect of Pseudoknot Base Pairing on Cotranscriptional Structural Switching of the Fluoride Riboswitch
Laura M Hertz,
Elise N White,
Konstantin Kuznedelov
et al.
Abstract:A central question in biology is how RNA sequence changes influence dynamic conformational changes during cotranscriptional folding. Here we investigated this question through the study of transcriptional fluoride riboswitches, non-coding RNAs that sense the fluoride anion through the coordinated folding and rearrangement of a pseudoknotted aptamer domain and a downstream intrinsic terminator expression platform. Using a combination ofE. coliRNA polymerasein vitrotranscription and cellular gene expression assa… Show more
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