The effect of protein concentration on the viscosity of a recombinant albumin solution formulation

Gonçalves, Andrea D; Alexander, Cameron; Roberts, Clive J.; Spain, Sebastian G.; Uddin, Shahid; Allen, Stephanie

doi:10.1039/c5ra21068b

Cited by 41 publications

(44 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a cell grows, mass is accumulated and there is an increase in the physical size, with the largest contribution to cellular dry mass being typically from proteins 40 . It is likely that the viscous component of cell behavior is influenced by the increase in proteins in the cytoplasm 41 , 42 . We observe a consistent and steady increase in our measurements at mitosis against interphase ( p ≈ 0.001) as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous time-varying viscosity, elasticity, and mass measurements of single adherent cancer cells across cell cycle

Adeniba

Corbin

Ganguli

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Biophysical studies on single cells have linked cell mechanics to physiology, functionality and disease. Evaluation of mass and viscoelasticity versus cell cycle can provide further insights into cell cycle progression and the uncontrolled proliferation of cancer. Using our pedestal microelectromechanical systems resonant sensors, we have developed a non-contact interferometric measurement technique that simultaneously tracks the dynamic changes in the viscoelastic moduli and mass of adherent colon (HT-29) and breast cancer (MCF-7) cells from the interphase through mitosis and then to the cytokinesis stages of their growth cycle. We show that by combining three optomechanical parameters in an optical path length equation and a two-degree-of-freedom model, we can simultaneously extract the viscoelasticity and mass as a function of the nano-scaled membrane fluctuation of each adherent cell. Our measurements are able to discern between soft and stiff cells across the cell cycle and demonstrated sharp viscoelastic changes due to cortical stiffening around mitosis. Cell rounding before division can be detected by measurement of mechanical coupling between the cells and the sensors. Our measurement device and method can provide for new insights into the mechanics of single adherent cells versus time.

show abstract

Section: Discussionmentioning

confidence: 99%

Simultaneous time-varying viscosity, elasticity, and mass measurements of single adherent cancer cells across cell cycle

Adeniba

Corbin

Ganguli

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Typical therapeutic mAbs formulations have concentrations around 100 mg/mL [2, 3], higher concentrations may yield prohibitively high viscosities. It is clear that the high viscosities of antibody solutions mostly arise from protein–protein interactions [4–14]. But, it is not yet clear how to rationally design formulations that can both maximize efficacy (protein concentration) and minimize viscosity [15, 16].…”

Section: Introductionmentioning

confidence: 99%

Controlling the viscosities of antibody solutions through control of their binding sites

Kastelic

Dill

Kalyuzhnyi

et al. 2018

Journal of Molecular Liquids

110

View full text Add to dashboard Cite

For biotechnological drugs, it is desirable to formulate antibody solutions with low viscosities. We go beyond previous colloid theories in treating protein–protein self–association of molecules that are antibody–shaped and flexible and have spatially specific binding sites. We consider interactions either through fragment antigen (Fab–Fab) or fragment crystalizable (Fab–Fc) binding. Wertheim's theory is adapted to compute the cluster–size distributions, viscosities, second virial coefficients, and Huggins coefficients, as functions of antibody concentration. We find that the aggregation properties of concentrated solutions can be anticipated from simpler–to–measure dilute solutions. A principal finding is that aggregation is controllable, in principle, through modifying the antibody itself, and not just the solution it is dissolved in. In particular: (i) monospecific antibodies having two identical Fab arms can form linear chains with intermediate viscosities. (ii) Bispecific antibodies having different Fab arms can, in some cases, only dimerize, having low viscosities. (iii) Arm–to–Fc binding allows for three binding partners, leading to networks and high viscosities.

show abstract

“…As the particle size becomes finer, the viscosity becomes lower. However, the apparent viscosity starts to increase again at 2% w/w, which might be due to the increment of the protein–protein aggregations of the unabsorbed protein in a continuous phase of the high SC concentration (Gonçalves et al, ).…”

Section: Resultsmentioning

confidence: 99%

Effect of sodium caseinate concentration and sonication amplitude on the stability and physical characteristics of homogenized coconut milk

Abdullah

Taip

Kamal

et al. 2018

J Food Process Preserv

View full text Add to dashboard Cite

The ultrasonic homogenization of coconut milk with added sodium caseinate (SC) was carried out to reduce the fat globules size and stabilize the emulsion. The coconut milk was mixed with SC at different concentrations from 0.5% to 2% w/w. The sonication was conducted at different sonication amplitudes (60%, 80% and 100%). The primary and effective droplet size, viscosity, homogenization efficiency and free fat content of the homogenized emulsion were characterized. Ultrasonic homogenization significantly reduced the average particle size to lower than half of its original size and reached to sub‐micron range size. Increasing the SC concentration positively reduced both the creaming index and free fat content without significant change in apparent viscosity. In this study, the changes in the physical characteristics of the emulsion were more affected by the SC concentration than the amplitude setting percentage. Nevertheless, both parameters were important in the formation of submicron‐sized droplets. Practical applications Coconut milk is highly unstable and vulnerable to oxidation. Due to its low stability, it can easily separate into two phases. Instability of the emulsion leads to the instability of the spray‐dried product. The homogenization of coconut milk fat with emulsifiers is widely conducted to increase the emulsion stability. The homogenization process will reduce the fat globules size while the emulsifier will lower the interfacial tension between the oil and water phases. However, sufficient power and amount of emulsifier are required in order to achieve high stability of the emulsion. The effects of the sonication amplitude and SC concentration on the stability of the coconut milk were investigated. The detailed knowledge obtained from this study on the optimum sonication amplitude and SC concentration can be utilized to improve the emulsion stability while taking food safety into consideration. Additionally, the physical characteristics of the emulsion are also determined, and results are found to be promising for the production of high stability emulsions.

show abstract

The effect of protein concentration on the viscosity of a recombinant albumin solution formulation

Cited by 41 publications

References 47 publications

Simultaneous time-varying viscosity, elasticity, and mass measurements of single adherent cancer cells across cell cycle

Simultaneous time-varying viscosity, elasticity, and mass measurements of single adherent cancer cells across cell cycle

Controlling the viscosities of antibody solutions through control of their binding sites

Effect of sodium caseinate concentration and sonication amplitude on the stability and physical characteristics of homogenized coconut milk

Contact Info

Product

Resources

About