The effect of prostaglandin inhibitors on the laser-induced disruption of the blood aqueous barrier in the rabbit

Schrems, W.; Dorp, H. P. van; Mager, S.

doi:10.1007/bf02133806

Cited by 12 publications

(5 citation statements)

References 7 publications

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“…The changes of aqueous protein and aqueous flare response in laser treated eyes in the present study are similar to previous observations (Kim & Lee 1995; Schrems et al 1983a) and are as expected after blood‐aqueous barrier breakdown after laser irradiation.…”

Section: Discussionsupporting

confidence: 92%

“…It is well documented that laser irradiation of the iris results in blood‐aqueous barrier breakdown with symptoms like protein leakage to the anterior chamber and an aqueous flare response (Kim & Lee 1995; Unger 1990; Schrems et al 1983a, 1983b) but, to our knowledge, there are no previous reports on hyaluronan changes after laser irradiation.…”

Section: Discussionmentioning

confidence: 98%

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Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Koralewska-Makár

Johnsson

Bruun

et al. 2001

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To monitor changes of endogenous hyaluronan in the iris tissue and aqueous humor after an isolated trauma to the iris by argon laser irradiation of the anterior surface of the iris. Methods: Iris and aqueous hyaluronan concentrations in rabbit were measured with a radiometric assay at different time points after laser irradiation. Results: Total hyaluronan content in iris tissue increased 3-fold to a peak concentration of 71-72 mg/g at 1 and 2 days after laser treatment. Aqueous hyaluronan increased to a maximum of about 1.6 mg/ml at 2 h and 12 h after laser irradiation of the iris. Conclusions:The iris tissue responds with increased hyluronan synthesis to an isolated iris argon laser irradiation and it seems to be the most important source of aqueous hyaluronan.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Koralewska-Makár

Johnsson

Bruun

et al. 2001

Acta Ophthalmologica Scandinavica

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“…The efficacy of prostaglandin inhibitors in various inflammatory, mechanical, or chemical alterations of the blood-aqueous barrier is well established in the scientific literature (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). The present experimental study was designed to evaluate the preventive effects of different topically applied prostaglandin inhibitors on the barrier function after laser trauma.…”

Section: Introductionmentioning

confidence: 99%

The effect of topically applied prostaglandin inhibitors on the laser-induced disruption of the blood aqueous barrier

Schrems¹,

Dorp

Mager³

1985

Doc Ophthalmol

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The therapeutic effect of topically applied prostaglandin inhibitors on the laser-induced disruption of the blood-aqueous barrier was investigated in six series of five rabbits each. One series was not coagulated and served as baseline, and in a reference group laser coagulation was performed without pretreatment with a prostaglandin inhibitor. In four series the iris laser coagulation of the left eyes was preceded by topical application of a prostaglandin inhibitor. The right eyes served as controls for the contralateral effect on the blood aqueous barrier. After laser coagulation the intraocular pressure was monitored at 10-min intervals, and the anterior chamber was tapped for analysis of the protein concentration and the lactate dehydrogenase activity. Pretreatment with dexamethasone eyedrops and indomethacin eyedrops markedly blocked the laser-induced disruption of the blood-aqueous barrier. The level of protein concentration in the aqueous humor after laser coagulation was much less after pretreatment with dexamethasone or indomethacin eyedrops. The effect was significant, both for the laser-treated eyes and for the noncoagulated fellow eyes (p less than 0.025). The subconjunctival pretreatment with dexamethasone 1 or 24 h before laser coagulation had no significant effect with respect to the protection of the blood aqueous barrier.

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“…The cause of the laser induced transient rise in IOP is believed to be due to breakdown of blood aqueous barrier as a result of prostaglandins (PG) released by laser treatment (5,9,11,12,18). Weinreb, et al (18) have also reported an elevated aqueous PGE2 level following laser trauma to the iris in pigmented rabbits.…”

Section: Discussionmentioning

confidence: 99%

“…The high degree of variability between animals is likely due to difficulty in controlling the intensity of the insult. In contrast, laser irradiation of the iris (5,11,12,18), although in need of refinement, would appear to provide a unique approach to the quantitative assessment of inhibition of prostaglandin mediated responses.…”

Section: Introductionmentioning

confidence: 99%

Argon Laser-Induced Ocular Hypertension: Animal Model of Ocular Inflammation

Gherezghiher

Koss²

1989

Journal of Ocular Pharmacology and Therapeutics

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There is substantial evidence that prostaglandins (PG's) mediate acute elevations of intraocular pressure (IOP) due to laser irradiation of the iris. In the present study, we determined the optimal laser parameters needed to produce the maximal rise in IOP in anesthetized rabbits. Application of argon laser (0.75 watts, 0.5 sec. duration, and 8 spots of 500 urn size) to the iris of pigmented rabbits elicited an acute rise in IOP and miosis accompanied by an increase in aqueous protein concentration. This response was followed by a delayed ocular hypotension that lasted for more than 3 days and returned to baseline by day 5. The onset of ocular hypertension occurred within 15 min. and remained elevated for at least 90 min.The laser induced rise in IOP was related more to the number of laser spots applied to the iris than to the intensity of the total laser energy utilized. Pretreatment with either indomethacin or flurbiprofen produced a dose dependent inhibition of ocular hypertensive response and significantly reduced aqueous protein concentration.Indomethacin (0.01-30 mg/kg) was administered intraperitonially 1 hr. before laser treatment and flurbiprofen (0.03-0.3%) was administered topically 45 and 15 min. prior to laser application. The hypertensive response was reduced by more than 90% of control after both drugs, whereas the delayed hypotension was significantly antagonized only at the highest dose of indomethacin.Neither indomethacin nor flurbiprofen altered the maximum miotic response to laser irradiation of the iris. However, 0.3% flurbiprofen and 30 mg/kg indomethacin significantly increased the recovery rate of the miotic pupil. The results of this study support the contention that argon laser induced ocular hypertension may provide a quantitative assessment of inhibition of prostaglandin mediated responses.

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The effect of prostaglandin inhibitors on the laser-induced disruption of the blood aqueous barrier in the rabbit

Cited by 12 publications

References 7 publications

Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

Influence of laser irradiation on endogenous hyaluronan in rabbit iris and aqueous humor

The effect of topically applied prostaglandin inhibitors on the laser-induced disruption of the blood aqueous barrier

Argon Laser-Induced Ocular Hypertension: Animal Model of Ocular Inflammation

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