The Effect of Prolonged Light Exposure on the Effectiveness of Phytochrome in Anthocyanin Synthesis in Tomato Seedlings*

Drumm‐Herrel, H.; Mohr, Hans

doi:10.1111/j.1751-1097.1982.tb03838.x

Cited by 38 publications

(22 citation statements)

References 7 publications

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“…These results are consistent with previous findings (3-5, 10, 11, 19-22). Blue pretreatments are more effective than R and FR ones in increasing AR (Table III), another observation consistent with previous findings (3)(4)(5)(19)(20)(21)(22). The effects of BL pretreatments on AR are enhanced by adding R and reduced by adding FR during the pretreatments (Table III).…”

Section: Resultssupporting

confidence: 81%

Cryptochrome, Phytochrome, and Anthocyanin Production

Mancinelli

Rossi²,

Moroni³

1991

Plant Physiol.

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Anthocyanin production in cabbage (Brassica oleracea L.) and tomato (Lycopersicon esculentum Mill.) seedlings exposed to prolonged irradiations was studied under conditions that allowed discrimination, within certain limits, between the contribution of cryptochrome and phytochrome in the photoregulation of the response. The results of the study provide confirming evidence for the involvement of cryptochrome and direct evidence for a significant contribution of cryptochrome to the fluence rate dependence of the response to blue. The results provide some preliminary, direct indication for an interaction between cryptochrome and phytochrome in the photoregulation of anthocyanin production in seedlings exposed to the prolonged irradiations required for a high level of expression of the response. The type and degree of interaction between the two photoreceptors vary significantly, depending on the species and experimental conditions. Light-dependent anthocyanin production requires prolonged exposures to relatively high fluence rates of visible and near-visible radiation for a high level of response expression (10, 11). The extent of the response is a function of light quality, fluence rate, and exposure duration; action peaks have been found in the UV, BL,3 R, and FR spectral regions. The relative efficiencies of these regions vary significantly, depending on the species and experimental conditions (10,11).Anthocyanin production and other plant photomorphogenic responses to prolonged R and FR irradiations are mediated by phytochrome (10,11,16 acting or acting independently of one another (2,7,(19)(20)(21)24).The unknown nature of the UV-B-photoreceptor and cryptochrome and the fact that UV and BL excite not only these two photoreceptors, but also phytochrome, complicate studies on photoreceptor involvement and interaction in the mediation of responses to UV and BL (7,14). The selection of light treatments that can be used to discriminate the action of different photoreceptors is based on criteria ( 18,24) that take into account the differences between the known (phytochrome) and the inferred (cryptochrome and UV-B-photoreceptor) spectral properties of the photoreceptors.Both cryptochrome and phytochrome are involved in the photoregulation of anthocyanin production in young seedlings of cabbage (Brassica oleracea L.) and tomato (Lycopersicon esculentum Mill.) exposed to prolonged irradiations. Phytochrome involvement has been known for a long time (10, 11). Evidence for cryptochrome involvement (25) was obtained recently: BL was found to be significantly more effective than RF (a mixture of R and FR containing no BL) under conditions in which BL and RF maintained the same state of phytochrome in terms of P, k, and H ((PBL = (PRF, kBL = kRF, and HBL = HRF). Since RF excites phytochrome, but not cryptochrome, and BL excites both photoreceptors, the differences in anthocyanin production between BL and RF treatments that maintain the same state of phytochrome can be reasonably attributed to an involvement of crypt...

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Section: Resultssupporting

confidence: 81%

Cryptochrome, Phytochrome, and Anthocyanin Production

Mancinelli

Rossi²,

Moroni³

1991

Plant Physiol.

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“…phyA and a UVB-absorbing photoreceptor. Furthermore it confirms the idea that the accumulation of anthocyanins in the hypocotyl of tomato can only express itself fully in the presence of a high Pfr/Pr ratio (DRUMM-HERREL & MoHR, 1982). On the other hand the highest increase of responsiveness to UV radiation was induced by continuous irradiation with LPS light from sowing time.…”

Section: Anthocyanin Accumulationsupporting

confidence: 79%

Photomorphogenic responses to UV radiation IV: a comparative study of UVB effects on growth and pigment accumulation in etiolated and de-etiolated hypocotyls of wild-type andaureamutant of tomatoLycopersicon esculentumMill)

Lercari

Bertram

1997

Plant Biosystems - An International Journal Dealing with all As

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“…Phytochrome interacts with blue light receptors to inhibit the elongation of tomato hypocotyls (Thomas and Dickinson, 1979) and also to stimulate anthocyanin synthesis in the subepidermal cells of the hypocotyl (Drumm-Herrel and Mohr, 1982). Phytochrome deficiency in tomato, caused by lesions at the aurea locus (Koornneef et al, 1985), has the opposite effect.…”

Section: Discussionmentioning

confidence: 99%

Oat Phytochrome Is Biologically Active in Transgenic Tomatoes.

1989

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To determine the functional homology between phytochromes from evolutionarily divergent species, we used the cauliflower mosaic virus 35s promoter to express a monocot (oat) phytochrome cDNA in a dicot plant (tomato). lmmunoblot analysis shows that more than 50% of the transgenic tomato plants synthesize the full-length oat phytochrome polypeptide. Moreover, leaves of light-grown transgenic plants contain appreciably less oat phytochrome than leaves from dark-adapted plants, and etiolated R I transgenic seedlings have higher levels of spectrally active phytochrome than wild-type tomato seedlings in direct proportion to the leve1 of immunochemically detectable oat polypeptide present. These data suggest that the heterologous oat polypeptide carries a functional chromophore, allowing reversible photoconversion between the two forms of the molecule, and that the far-red absorbing form (Pfr) is recognized and selectively degraded by the Pfr-specific degradative machinery in the dicot cell. The overexpression of oat phytochrome has pleiotropic, phenotypic consequences at all major phases of the life cycle. Adult transgenic tomato plants expressing high levels of the oat protein tend to be dwarfed, with dark green foliage and fruits. R1 transgenic seedlings have short hypocotyls with elevated anthocyanin contents. We conclude that a monocot phytochrome can be synthesized and correctly processed to a biologically active form in a dicot cell, and that the transduction pathway components that interact with the photoreceptor are evolutionarily conserved.

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The Effect of Prolonged Light Exposure on the Effectiveness of Phytochrome in Anthocyanin Synthesis in Tomato Seedlings*

Cited by 38 publications

References 7 publications

Cryptochrome, Phytochrome, and Anthocyanin Production

Cryptochrome, Phytochrome, and Anthocyanin Production

Photomorphogenic responses to UV radiation IV: a comparative study of UVB effects on growth and pigment accumulation in etiolated and de-etiolated hypocotyls of wild-type andaureamutant of tomatoLycopersicon esculentumMill)

Oat Phytochrome Is Biologically Active in Transgenic Tomatoes.

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