The effect of progesterone on the electron emission and degradation of testosterone

Abstract

Based on recent findings that hormones can emit electrons () from their excited singlet state in polar media, it was of importance to study a possible mutual interaction of progesterone (PRG) and testosterone (TES) in this respect. Hormones of highest purity were dissolved in an air-free mixture of 40% triply distilled water and 60% ethanol, because the hormones are unsoluble in water. As energy source for substrate excitation in singlet state served a monochromatic UV-light (254 nm), the emitted electrons were scavenged by chloroethanol, whereby the quantum yield of produced Cl⁻ ions, Q (Cl⁻), is equal to Q(e⁻(aq)). Hormone degradation initiated by the electron emission was studied by HPLC method, using a Zorbax Eclipse XDB-C18 column (150 mm x 4.6 mm, 5 μm). The quantum yield of emitted e⁻(aq), Q(e⁻(aq)), from TES was 3.6 times higher than that from PRG, which is explained by the different molecular structures of the hormones. Observed 2nd and 3rd maxima of electron emission indicate the ability of TES and PRG products to also eject e⁻(aq), but with lower yield. It can be stated that a part of the emitted electrons from TES are consumed by PRG⁺ leading to a partial regeneration of hormone. The present results offer a deeper insight in the biological behavior of hormones.

Hormones: electron emission, communication, mutual interaction, regeneration, metabolites, carcinogenesis and receptor action

“…The observed effect of PRG on the strong reduction of e aq − yield of TES in a mixture of both was partly explained by the above-mentioned hypothesis [17] . TES (based on its molecular structure) emits a higher e aq − yield, Q(e aq − ) = 0.0670, compared to that of PRG, Q(e aq − ) = 0.0134, (Table 1).…”

“…Based on the similarity of the molecular structure, the mechanisms of e aq − emission from 17 α -OHPR, 21 α -OHPRG and corticosterone (CORT) is very likely to those of PRG and TES discussed above [8,9,11,17,18] . A radical cation (R • + ) is formed in all cases, because a part of ejected e aq − results from ring A of the corresponding hormone.…”

“…The electron transfer from TES to PRG [17] inspired further experimental studies concerning a possible regeneration of the hormone transients originating from the e aq − ejection. The hormone regeneration was achieved by electron transfer from a potent electron donor-like vitamin C (Vit C) during the life time (status nascendistate) of the hormone transients.…”

Endogenous Photosensitizers in Human Skin

“…When electronically excited to its singlet state by monochromatic UV light, dopamine (DA) can emit solvated electrons that then react with it in the ground state. 372,373 Polydopamines (PDAs) are polymers widely used as models of materials resembling melanin. 374 Upon oxidation by the hydroxyl radical, PDA produces fluorescent polydopamine dots.…”