The effect of prior thecal puncture on cerebrospinal fluid analytes in normal adult horses

Abstract

Background: Serial cerebrospinal fluid (CSF) analysis might be required in clinical neurologic disease. The effect of lumbosacral (LS) or cervical (C1-C2) centesis on subsequent CSF cytologic analyses has not been investigated in horses. Objective: To evaluate the effect of thecal puncture on subsequent CSF analyses Animals: Ten healthy adult horses. Methods: Prospective study. Horses were randomly assigned to undergo CSF collection twice, 14 days apart, from either the C1-C2 or LS space. After a 4-month washout period, CSF collection was repeated from the alternate site. Continuous data were analyzed using linear mixed-effects models and count data using mixed-effects negative binomial regression. Statistical significance was set at P < .05. Results: There was no significant effect of collection day (day 0 or day 14) for any CSF analytes, including protein concentration (C1-C2: 45 [95% CI: 33-57] mg/dL day

Comparison of atlantoaxial and lumbosacral cerebrospinal fluid centesis techniques in South American camelids

“…In a study in horses, cytological changes were not noted in samples obtained by serial collection with a 2-week interval between collections. 18 In contrast, a study performed in healthy human volunteers indicated that repeated CSF centesis within a 2-week interval caused alterations in the TNCC of the collected samples, 23 which is similar to our study. Repeated sampling may have induced an inflammatory response in the CNS, which could affect the TNCC of the CSF.…”

“…In a previous study in horses, an average of 1.1 attempts was necessary for successful collection of CSF by AA centesis and in another study the median was 1 attempt, 14 , 18 which is comparable to the median of 1 attempt for AA centesis in our study. The highest number of attempts and redirections for successful AA centesis, resulting in the longest collection time recorded in our study, occurred with the novice operator attempting the AA centesis technique for the second time, which also was reflected in the significant interaction term identified for the number of attempts.…”

“…Occasionally, repeated, or serial CSF centesis procedures can be clinically necessary. In a study in horses, cytological changes were not noted in samples obtained by serial collection with a 2‐week interval between collections 18 . In contrast, a study performed in healthy human volunteers indicated that repeated CSF centesis within a 2‐week interval caused alterations in the TNCC of the collected samples, 23 which is similar to our study.…”