The effect of presence of facultative bacteria species on semen and sperm quality of men seeking fertility care

Enwuru, Christian A.; Iwalokun, BA; Enwuru, Veronica N.; Ezechi, Oliver; Oluwadun, Afolabi

doi:10.1016/j.afju.2016.03.010

Cited by 26 publications

(26 citation statements)

References 20 publications

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“…Also, those whose sperms had ≤30% normal morphology (oval heads) had higher mean pus cells than those whose sperms had >30% normal morphology (oval head) which agrees with the findings of Khan et al [29]. The observed inverse relationship between motility and pus cells accord with the findings of Enwuru et al, [20].…”

Section: Discussionsupporting

confidence: 83%

“…To conclude, this study leans towards the belief that pyospermia affects the quality of sperm because high pus cell counts were seen more in azoospermic than in oligoospermic or normospermic patients, contrary to what Enwuru et al [20] reported. This appears to be a confirmation that pyospermia could be one of the characteristic sources of infertility in the human male.…”

Section: Resultscontrasting

confidence: 52%

“…Thirdly, there were 346 (38.1%) men in the oligozoospermic cluster,a figure lower than the 52.5% oligozoospermic patients reported by Enwuru et al [20].…”

Section: Discussioncontrasting

confidence: 42%

“…Few studies in sub-Saharan Africa have delved into the issue of pyospermia and male infertility. Enwuru et al, [20] reported that normozoospermic males with pyospermia had lower sperm concentration compared with those without contamination, while Onemu and Ibeh [21] concluded that pathogenic pyospermia, consequent upon breach in the integrity of the blood-testes barrier, may provide early warning signals of impairment of male fertility. However, these studies looked at pyospermia mainly from icrobiologyic perspective and did not fully relate pyospermia to age group, body mass index, progressive motility, and normal sperm morphology consisting of sperms with oval heads.…”

Section: A B Ajayi Et Almentioning

confidence: 99%

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Pyospermia in Men Seeking Assisted Reproduction Technology

Ajayi¹,

Afolabi²,

Ajayi³

et al. 2018

OJU

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Introduction: Pyospermia is often detected in semen, especially when investigating for male infertility. Pyospermia, in a significantly large number is often correlated with inferior sperm parameters and reduced fertility. Pyospermia may be a factor of a fundamental genitourinary infection. This study assessed the relationship between pus cells and semen parameters in infertile Nigerian males. Subjects and Methods: A cross-sectional descriptive study was carried out at Nordica Fertility Center, Lagos, Nigeria, from 2004 to 2009. A total of 907 subjects were analyzed. Subjects ages (years) were categorized into ≤30, BMI into <18.5 (underweight), and ≥30 (obese) and pus cells into none, 1 -2 and ≥3. Results: Leucocytopenia of ≥3 per high power field (/hpf) occurred in 67 (7.4%) men. The overall means (±sd) of pus cells was 1.4 (0.9), 1.4 (0.7), 1.4 (0.7) and 1.6 (1.6) per high power field in all, normospermia, oligoospermic and azoospermic clusters of patients respectively. Mean (±sd) pus cells in semen was 6.0 (4.0), the highest, among obese (BMI ≥ 30) azoospermic patients. The proportion of subjects with pyospermia of 1 -2 was highest (323/346, 93.3%) among oligoospermic patients while that with pyospermia of ≥3 was highest (10/109, 9.2%) among azoospermic patients. Obese azoospermic patients were 1.8 times as likely to have ≥3/hpf pyospermia compared to normal normospermic patients (x² = 0.05, P-value = 0.51, OR = 1.82, 95% CI: 0.30, 11.02). Mean progressive motility (%) was significantly reduced (t = 1.95, P-value = 0.03) among patients with ≥3/hpf pyospermia (33.75 ± 19.23) compared to those with 1 -2/hpf pyospermia (38.58 ± 21.63). Patients with sperm oval head ≤ 30% had significantly higher pyospermia (t = 2.22, P-value = 0.013) than those with sperm oval head of >30%. Conclusion: Elevated pus cell counts were observed among obese

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Section: Discussionsupporting

confidence: 83%

Section: Resultscontrasting

confidence: 52%

“…Thirdly, there were 346 (38.1%) men in the oligozoospermic cluster,a figure lower than the 52.5% oligozoospermic patients reported by Enwuru et al [20].…”

Section: Discussioncontrasting

confidence: 42%

Section: A B Ajayi Et Almentioning

confidence: 99%

See 2 more Smart Citations

Pyospermia in Men Seeking Assisted Reproduction Technology

Ajayi¹,

Afolabi²,

Ajayi³

et al. 2018

OJU

View full text Add to dashboard Cite

show abstract

“…This could be due to the fact that those patients with primary infertility are usually more concerned in seeking medical advice than men with secondary infertility (Shafi et al, 2014). Enwuru et al investigated 162 infertile and 54 fertile patients and found that patients with leukocytospermia had decreased total sperm count and decreased sperm motility (Enwuru et al, 2016). In contrast to previous studies, Vilvanathan et al had not obtained any positive association of asymptomatic leukocytospermia and infertility (Vilvanathan et al, 2016).…”

Section: Discussionmentioning

confidence: 96%

Association between leukocytospermia and semen interleukin‐6 and tumor necrosis factor‐alpha in infertile men

et al. 2018

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Seminal cytokines were previously reported to adversely affect process of spermatogenesis and ultimately induce poor semen quality. However, association between both IL-6 and TNF-α and leukocytospermia was not yet settled. The aim of this study was to evaluate the association between leukocytospermia and levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in semen of infertile men. This cross-sectional study included 100 age-matched (≥18-45 years.) men. Participants were divided into four groups with 25 patients in each group: Group (A) - infertile patients with leukocytospermia and normal other semen parameters; Group (B) - infertile patients with leukocytospermia and abnormal semen parameters; Group (C) - infertile patients with oligospermia and/or asthenospermia and/or teratospermia but with no leukocytospermia; Group (D) - fertile patients with normal semen parameters and without leukocytospermia. All patients were assessed by detailed medical, sexual, fertility history, and complete physical examination. Laboratory assessment included hormonal and semen analysis and assessment of IL-6 and TNF-α in semen plasma. There were significant differences among the study groups regarding total sperm count, sperm concentration, and progressive motility (p < 0.05 for each). There was significant increase in semen WBC counts in groups A and B vs. groups C and D (p = 0.003). There were significant associations between increase levels of WBCs ≥ 5/HPF and decrease levels of total sperm count (p = 0.023), sperm concentration (p = 0.001), and sperm progressive motility (p = 0.02). There were significant upregulations in mean level of IL-6 (p = 0.001) and mean level of TNF-α (p = 0.003) in groups A and B vs. groups C and D. Overall, leukocytospermia is associated with reduction in sperm count, progressive motility and further upregulation of seminal IL-6 and TNF-α. The effect of treatment of leukocytospermia on the level of seminal cytokines is important point of future research.

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