The effect of preincubation of HeLa cell nuclei with ATP on the degradation of mononucleosomal DNA by micrococcal nuclease

Pentz, Margaret; Vatev, Radka; Goldthwait, David A.

doi:10.1093/nar/14.13.5513

Cited by 3 publications

(3 citation statements)

References 41 publications

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“…Following electrophoresis, the gels were applied to Kodak XAR-5 film for 0.5-3 days, and the resulting autoradiographic signals were quantitated by scanning densitometry (Transidyne General). Alternatively, the regions of the gel producing major autoradiographic signals were excised, and the radioactivity was determined by a liquid scintillation spectrometer (Pentz et al, 1986).…”

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confidence: 99%

Expression and stability of c-sis mRNA in human glioblastoma cells

Press¹,

Samols²,

Goldthwait³

1988

Biochemistry

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The production of platelet-derived growth factor like (PDGF-like) material by glioblastomas may be involved in the conversion of normal cells to tumor cells. In an investigation of this problem, we have examined some of the properties of the platelet-derived growth factor B-chain mRNA (c-sis mRNA) by a sensitive and quantitative RNA-RNA solution hybridization method. In 5 out of 8 human glioblastoma cell lines, c-sis mRNA was present, and in the line with the highest level, there were approximately 4-10 molecules per cell. The half-lives of the c-sis mRNA in two glioblastoma cell lines were 2.6 and 3.4 h, while in human umbilical vein endothelial (HUVE) and bladder carcinoma (T24) cells they were 1.6 and 2.5 h, respectively. Inhibiting protein synthesis produced no significant alteration of the c-sis mRNA half-lives in the glioblastoma or HUVE cells. The A-U-rich sequence at the 3' end of the c-sis mRNA therefore does not appear to affect the mRNA stability in the presence of cycloheximide as it does in other transcripts. The similarity of the c-sis mRNA half-lives in normal and tumor cells suggests that regulation of stability of c-sis mRNA is not a major factor in tumorigenesis in the glioblastoma cell lines examined.

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confidence: 99%

Expression and stability of c-sis mRNA in human glioblastoma cells

Press¹,

Samols²,

Goldthwait³

1988

Biochemistry

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“…It is unclear whether AMPK acts directly on the components of DNA double strand break repair pathways. However, we did observe that ATP supplementation could ameliorate the DNA repair defect caused by ATIC inhibition and there is a long history of reports that highlight the ATP dependence of repair of ultraviolet or ionizing radiation damage to DNA [3,4,6,15,24,33,35]. In addition, prior studies have shown that inhibition of AMPK activity can result in radioresistance [31,39] while AMPK activators can augment cell killing by ionizing radiation [7,26,28,36,44].…”

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confidence: 88%

Identification of ATIC as a Novel Target for Chemoradiosensitization

Liu

Paila

Teraoka

et al. 2018

International Journal of Radiation Oncology*Biology*Physics

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“…2a). This was an unexpected result, but nucleoside triphosphates have been shown to influence conformation of histones and unfolding of nucleosomes (33,34). It has been reported (14) that Tn3 transposase binds only to the Tn3 inverted repeat sequence in the presence of ATP, whereas DNA binding in the absence of ATP is both specific and nonspecific.…”

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Nucleoside triphosphate-dependent DNA-binding properties of mos protein.

Seth¹,

Priel²,

Woude³

1987

Proc. Natl. Acad. Sci. U.S.A.

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We have previously shown that the mos gene product, p40f°, produced in Escherichia coli binds ATP and has ATPase activity. In the present study, we investigated the DNA-binding properties of p4011S and two mos deletion mutant proteins. Nitrocellulose blot protein-DNA binding assaysshowed that p40otm binds DNA in the presence of Mg2+-ATP and certain other nucleoside triphosphates. Ninety percent of the p40'°"-bound DNA is dissociated if the complex is washed in the presence of 1 M NaCl or in the absence of ATP. (4,5,11), the SV40 large T antigen shows both sequence-specific and nonspecific binding to DNA sequences (8)(9)(10)12). Recently, the DNA sequence-specific binding of SV40 large T antigen was shown to be abolished in an ATP-dependent manner by the allosteric effect of purine nucleoside triphosphates (13).However, in another case, the Tn3 transposase has been shown to bind specifically to the Tn3 inverted repeat in the presence of ATP (14). The viral mos (v-mos) gene of Moloney murine sarcoma virus (Mo-MSV) encodes a 37-kDa env-mos fusion protein (15,16). The mos proteins expressed by Mo-MSV strains tsllO and 124 have been shown to have serine/threonine autophosphorylation activity (17, 18). The v-mos product has been localized to the cytoplasm in acutely infected and transformed cells and is present at extremely low levels (19). The low level of mos product in transformed cells makes the characterization of its biochemical properties difficult. Therefore, we expressed the mos product and several mos deletion mutant proteins in Escherichia coli by using derivatives of high-level-expression vectors pJL6 and pANH-1 (20, 21). The derivative containing the complete mos gene (pA28) directed the synthesis of p40os (22), whereas those vectors lacking either N-terminal (pANHA28) or C-terminal (pA28A8) moieties expressed truncated versions of p4Omos, called pl9moS and p25m0s, respectively (21,22). The mos protein has strong homology with the ATP-binding domain of the cAMP-dependent bovine protein kinase and members of the src kinase family of oncogene products (23). We have shown that purified p40mOS expressed in E. coli binds ATP and has ATPase activity (22 (11,25). The purified mos and deletion mutant proteins were resolved in a NaDodSO4/12% polyacrylamide gel and were transferred electrophoretically onto nitrocellulose paper (26). The paper was incubated with 1% non-fat dry milk (27) in 0.5 M NaCl/50 mM Tris HCl, pH 8. 3560The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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The effect of preincubation of HeLa cell nuclei with ATP on the degradation of mononucleosomal DNA by micrococcal nuclease

Cited by 3 publications

References 41 publications

Expression and stability of c-sis mRNA in human glioblastoma cells

Expression and stability of c-sis mRNA in human glioblastoma cells

Identification of ATIC as a Novel Target for Chemoradiosensitization

Nucleoside triphosphate-dependent DNA-binding properties of mos protein.

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