The effect of prehydrolysis and improved mixing on high-solids batch simultaneous saccharification and fermentation of spruce to ethanol

Hoyer, Kerstin; Galbe, Mats; Zacchi, Guido

doi:10.1016/j.procbio.2012.12.020

Cited by 62 publications

(25 citation statements)

References 14 publications

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“…These findings were in agreement with Romaní et al (2014) and Hoyer et al (2009), who reported better performance for SSF than PSSF. The PSSF process significantly increased the % cellulose conversion only when the % cellulose conversion and ethanol concentration were low in the SSF process, but otherwise, it did not have a significant effect, or even had a negative effect when a high cellulose conversion and ethanol content were obtained without a pre-hydrolysis step (Hoyer et al 2013).…”

Section: Pssf Vs Ssfmentioning

confidence: 92%

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Aliberti¹,

Ventorino²,

Robertiello³

et al. 2017

BioResources

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Arundo donax was used to investigate the effect of the enzyme and substrate concentrations on hydrolysis, pre-hydrolysis and simultaneous saccharification and fermentation (PSSF), in comparison to simultaneous saccharification and fermentation (SSF). Hydrolysis was performed at 37 and 50 °C. At the highest biomass (10%) and enzyme (69.6 FPU/g cellulose) loadings, the highest glucose concentration (32.4 g/L) was obtained (at 50 °C). SSF resulted in a cellulose conversion (91.9%) and an ethanol concentration (19.8 g/L) higher than what was obtained using PSSF at 37 °C (86.9% and 18.8 g/L, respectively) and PSSF at 50 °C (81.6% and 17.7 g/L, respectively). A positive correlation between the cellulase concentration, cellulose conversion, and ethanol content was observed. In PSSF, the increase in the solids loadings caused a reduction in the % cellulose conversion, but the ethanol concentration in PSSF and SSF increased. SSF appeared to be the most advantageous process for bioethanol production from A. donax.

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Section: Pssf Vs Ssfmentioning

confidence: 92%

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Aliberti¹,

Ventorino²,

Robertiello³

et al. 2017

BioResources

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“…The results from this study obtained by SPORL were compared with a recent study based on SO 2 steam explosion of spruce ( Hoyer et al 2013 ) as presented in Table 3 . Despite the higher thermal energy input at 205 ° C in SO 2 steam explosion ( Zhu and Zhuang 2012 ), along with supplementation of nutrients and 50 times higher yeast loading, the SO 2 steam explosion study produced a lower ethanol titer of 47.8 g l -1 and the theoretical yield was 72% (based on wood glucan and mannan), whereas in the present SPORL study the yield were 52.2 g l -1 (82.5% of the theory).…”

Section: Ethanol Yield and Process Mass Balancementioning

confidence: 99%

“…Effective and commercially scalable pretreatment technologies are still a challenge in view of the difficult accessibility of wood to enzymes ( Lynd et al 2008 ;Zhu and Pan 2010 ). The research goals are high sugar yields, low toxicant formation, and low nonproductive cellulase binding by lignin to avoid solids washing and facilitate fermentation of pretreated whole slurry at high solids ( Pan et al 2005 ;Monavari et al 2010 ;Zhu and Pan 2010 ;Yamamoto et al 2011 ;Modenbach and Nokes 2012 ;Agarwal et al 2013 ;Hoyer et al 2013 ;Lan et al 2013a ;Tunc et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Pilot-scale demonstration of SPORL for bioconversion of lodgepole pine to bioethanol and lignosulfonate

Zhou¹,

Zhu

Gleisner

et al. 2015

Holzforschung

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The process sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) has been the focus of this study. Pilot-scale (50 kg) pretreatment of wood chips of lodgepole pine ( Pinus contorta Douglas ex Loudon) killed by mountain pine beetle ( Dendroctonus ponderosae Hopkins) were conducted at 165 ° C with a dilute sulfite solution of pH 2 for bioconversion to ethanol and lignosulfonate (LS). The pretreatment duration was optimized in laboratory bench scale experiments with a certain severity based on a combined hydrolysis factor (CHF). The sodium bisulfite loading was 8% and the liquor to wood ratio 3. The pretreated solids were disk milled together with the spent liquid and the resultant slurry with a 25% solids content was directly (without detoxification) submitted to a simultaneous enzymatic saccharification and fermentation (SSF) with Saccharomyces cerevisiae YRH400 at cellulase loading of 35 ml kg -1 of untreated wood. At solids loading of 20%, the alcohol yield was 288 l t -1 wood (with a final concentration of 52.2 g l -1 ), which corresponds to a 72.0% theoretical yield based on total glucan, mannan, and xylan. The LS from SPORL was highly sulfonated and its molecular weight was lower than that of a purified commercial softwood LS, and therefore it has a high potential as a directly marketable co-product.

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“…Additionally, a high enzyme dosage is required under these conditions (Zhang et al, 2006). To overcome these technical barriers, novel strategies have been demonstrated (Lan et al, 2013;Hoyer et al, 2013). Fed-batch schemes, as an alternative method of achieving high-solids loading has been investigated for its various advantages (Hodge et al, 2009;Rosgaard et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimization of high solids fed-batch saccharification of sugarcane bagasse based on system viscosity changes

Liu

Zhang

et al. 2015

Journal of Biotechnology

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The effect of prehydrolysis and improved mixing on high-solids batch simultaneous saccharification and fermentation of spruce to ethanol

Cited by 62 publications

References 14 publications

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Pilot-scale demonstration of SPORL for bioconversion of lodgepole pine to bioethanol and lignosulfonate

Optimization of high solids fed-batch saccharification of sugarcane bagasse based on system viscosity changes

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