The effect of pre-coating human bone marrow stromal cells with hydroxyapatite/amino acid nanoconjugates on osteogenesis

Babister, Jodie C.; Hails, Lauren A.; Oreffo, Richard O.C.; Davis, Sean A.; Mann, Stephen

doi:10.1016/j.biomaterials.2009.02.024

Cited by 18 publications

(18 citation statements)

References 25 publications

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“…Similar results were obtained by Jones et al [64] , where the ALPase activity for human osteoblast cells (HOB) was highest on d 14, after which a decrease was observed due to the beginning of mineralization in HOB. In an experiment by Babister et al [1] , both the activity and the transcription of ALPase in hMSCs were highest on d 21. Conversely, Degistirici et al [41] found that USSCs expressed the most active ALPase on d 7 during osteogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

“…The first approach is the administration of large amounts of osteogenically predifferentiated cells in microsphere form [1,36] . But this technique is hampered by the limited volume of microspheres administered into the area of defect and the risk of effluence into other, unfavorable sites.…”

Section: Discussionmentioning

confidence: 99%

“…Control over the thickness and composition of the porous nanofibers is an important aspect of this technique [10] . Hydroxyapatite (HA) composites have received a great deal of attention for bone regeneration because they possess excellent biodegradability, biocompatibility, osteoconductivity and direct bone-binding capability [1,3] . Yang et al [13] demonstrated that the incorporation of HA into PCL/gelatin nanofibers enhanced dental pulp stem cell differentiation toward an odontoblast-like phenotype in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…Current practice mainly relies on tissue repair by autograft and allograft or application of synthetic bone implants [1,2] . However, many limitations, such as donor morbidity, limited availability of tissue suppliers, potent immune response, disease transfer, or insufficient bioactivity, have led this field to tissue engineering as an excellent alternative approach [3][4][5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

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Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

et al. 2011

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Aim: Bioartificial bone tissue engineering is an increasingly popular technique to solve bone defect challenges. This study aimed to investigate the interactions between matrix composition and appropriate cell type, focusing on hydroxyapatite (HA), to achieve a more effective combination for bone regeneration. Methods: Human unrestricted somatic stem cells (USSCs) were isolated from placental cord blood. The cellular and molecular events during the osteo-induction of USSCs were evaluated for 21 d under the following conditions: (1) in basal culture, (2) supplemented with hydroxyapatite nanoparticle (nHA) suspension, and (3) seeded on electrospun aligned nanofibrous poly-ε-caprolactone/poly-L-lactic acid/nHA (PCL/PLLA/nHA) scaffolds. The scaffolds were characterized using scanning electron microscope (SEM), fourier transform infrared spectroscopy (FTIR) and tensile test. Results: Maintenance of USSCs for 21 d in basal or osteogenic culture resulted in significant increase in osteoblast differentiation. With nHA suspension, even soluble osteo-inductive additives were ineffective, probably due to induced apoptosis of the cells. In contrast to the hindrance of proliferation by nHA suspension, the scaffolds improved cell growth. The scaffolds mimic the nanostructure of natural bone matrix with the combination of PLLA/PCL (organic phase) and HA (inorganic phase) offering a favorable surface topography, which was demonstrated to possess suitable properties for supporting USSCs. Quantitative measurement of osteogenic markers, enzymatic activity and mineralization indicated that the scaffolds did not disturb, but enhanced the osteogenic potential of USSCs. Moreover, the alignment of the fibers led to cell orientation during cell growth. Conclusion:The results demonstrated the synergism of PCL/PLLA/nHA nanofibrous scaffolds and USSCs in the augmentation of osteogenic differentiation. Thus, nHA grafted into PCL/PLLA scaffolds can be a suitable choice for bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

et al. 2011

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“…The coating of human bone marrow stromal cells (HBMSC) with amino-acid functionalized HA nanoparticles leads to a significant increase in osteoblast differentiation when compared to uncoated cells [29]. The in vivo component of this study demonstrated comparable osteoid formation after 21 days in immunocompromised mice.…”

Section: Ceramicsmentioning

confidence: 88%

The Use of Ceramics as Bone Substitutes in Revision Hip Arthroplasty

Whitehouse

Blom

2009

Materials

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The number of grafting procedures, including those performed in primary and revision hip arthroplasty, continues to rise around the world. Demand for musculoskeletal donor tissue now outstrips supply. There is no single bone substitute that is ideal for all circumstances. Bone substitutes act as a scaffold and are usually osteoconductive. They are rarely osteoinductive; if they are, a molecular bond is formed between the graft and host bone, improving fixation and longevity. Bone graft substitutes are very rarely osteogenic. There is a growing body of clinical evidence supporting the use of bone graft substitutes in vivo for complex hip arthroplasty.

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Surface‐engineered hydroxyapatite nanocrystal/ poly(ε‐caprolactone) hybrid scaffolds for bone tissue engineering

Kim

2011

J of Applied Polymer Sci

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To achieve novel polymer/bioceramic composite scaffolds for use in materials for bone tissue engineering, we prepared organic/inorganic hybrid scaffolds composed of biodegradable poly(e-caprolactone) (PCL) and hydroxyapatite (HA), which has excellent biocompatibility with hard tissues and high osteoconductivity and bioactivity. To improve the interactions between the scaffolds and osteoblasts, we focused on surface-engineered, porous HA/ PCL scaffolds that had HA molecules on their surfaces and within them because of the biochemical affinity between the biotin and avidin molecules. The surface modification of HA nanocrystals was performed with two different methods. Using Fourier transform infrared, X-ray diffraction, and thermogravimetric analysis measurements, we found that surface-modified HA nanocrystals prepared with an ethylene glycol mediated coupling method showed a higher degree of coupling (%) than those prepared via a direct coupling method. HA/PCL hybrid scaffolds with a well-controlled porous architecture were fabricated with a gas-blowing/particle-leaching process. All HA/PCL scaffold samples exhibited approximately 80-85% porosity. As the HA concentration within the HA/PCL scaffolds increased, the porosity of the HA/PCL scaffolds gradually decreased. The homogeneous immobilization of biotin-conjugated HA nanocrystals on a three-dimensional, porous scaffold was observed with confocal microscopy. According to an in vitro cytotoxicity study, all scaffold samples exhibited greater than 80% cell viability, regardless of the HA/ PCL composition or preparation method.

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The effect of pre-coating human bone marrow stromal cells with hydroxyapatite/amino acid nanoconjugates on osteogenesis

Cited by 18 publications

References 25 publications

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

Effective combination of aligned nanocomposite nanofibers and human unrestricted somatic stem cells for bone tissue engineering

The Use of Ceramics as Bone Substitutes in Revision Hip Arthroplasty

Surface‐engineered hydroxyapatite nanocrystal/ poly(ε‐caprolactone) hybrid scaffolds for bone tissue engineering

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